Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,089,844	T→C	E4E (GAA→GAG)	frvR ←	putative transcriptional regulator FrvR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,089,844	0	T	C	100.0%	138.3 / NA	38	E4E (GAA→GAG)	frvR	putative transcriptional regulator FrvR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/16);  total (22/16)

GCTTTGCCGTTAAGGGTGAAGTTGAGATAGTCAATATCACGCAGGATGGTCCTGCCTGAAACGCCAGTCTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGTTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAACCCCGTATCAGCGTGAGCAGAGCTTCGTAATCGGCTTTTGAAATCATACCGCTGTTGGCGTG  >  NC_000913/4089710‑4089980                                                                                                                                       |                                                                                                                                         gCTTTGCCGTTAAGGGTGAAGTTGAGATAGTCAATATCACGCAGGATGGTCCTGCCTGAAACGCCAGTCTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGcgctcgtt                                                                                                                                     <  2:770757/140‑1 (MQ=255)     tGCCGTTAAGGGTGAAGTTGAGATAGTCAATATCACGCAGGATGGTCCTGCCTGAAACGCCAGTCTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGc                                                                                                                                 <  1:812792/140‑1 (MQ=255)     tGCCGTTAAGGGTGAAGTTGAGATAGTCAATATCACGCAGGATGGTCCTGCCTGAAACGCCAGTCTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGc                                                                                                                                 <  1:458937/140‑1 (MQ=255)           tAAGGGTGAAGTTGAGATAGTCAAAATCACACAGGATGGTCCTGCCTGAAACGCCAGTCTGATGCGCCAGCTCGCCAGGCGTGCGCGGCTGCTGCTCCAGCAGATCGACAATCTTTAACTGACGCTCGTTTAGCATTAAt                                                                                                                           >  2:585538/1‑140 (MQ=255)            aaGGGTGAAGTTGAGATAGTCAATATCACGCAGGATGGTCCTGCCTGAAACGCCAGTCTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATc                                                                                                                          <  1:805925/140‑1 (MQ=255)              gggTGAAGTTGAGATAGTCAATATCACGCAGGATGGTCCTGCCTGAAACGCCAGTCTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCa                                                                                                                        <  1:670809/140‑1 (MQ=255)               ggTGAAGTTGAGATAGTCAATATCACGCAGGATGGTCCTGCCTGAAACGCCAGTCTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCAc                                                                                                                       >  2:586736/1‑140 (MQ=255)                 tGAAGTTGAGATAGTCAATATCACGCAGGATGGTCCTGCCTGAAACGCCAGTCTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCt                                                                                                                     >  2:840329/1‑140 (MQ=255)                 tGAAGTTGAGATAGTCAATATCACGCAGGATGGTCCTGCCTGAAACGCCAGTCTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCt                                                                                                                     >  2:820788/1‑140 (MQ=255)                           aTAGTCAATATCACGCAGGATGGTCCTGCCTGAAACGCCAGTCTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTg                                                                                                           >  2:889693/1‑140 (MQ=255)                               tCAATATCACGCAGGATGGTCCTGCCTGAAACGCCAGTCTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTa                                                                                                       >  1:447077/1‑140 (MQ=255)                                        cGCAGGATGGTCCTGCCTGAAACGCCAGTCTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTg                                                                                              >  1:579012/1‑140 (MQ=255)                                         gCAGGATGGTCCTGCCTGAAACGCCAGTCTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGa                                                                                             >  1:1029265/1‑140 (MQ=255)                                             gATGGTCCTGCCTGAAACGCCAGTCTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACttt                                                                                         >  2:102775/1‑140 (MQ=255)                                               tGGTCCTGCCTGAAACGCCAGTCTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTtct                                                                                       >  1:946124/1‑140 (MQ=255)                                                       cctgAAACGCCAGTCTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGtt                                                                               >  2:541344/1‑140 (MQ=255)                                                         tgAAACGCCAGTCTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTaa                                                                             >  2:388023/1‑140 (MQ=255)                                                           aaaCGCCAGTCTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAgg                                                                           >  1:500558/1‑140 (MQ=255)                                                            aaCGCCAGTCTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGgt                                                                          >  2:188101/1‑140 (MQ=255)                                                              cGCCAGTCTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGgtcg                                                                        <  1:889693/140‑1 (MQ=255)                                                                     cTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGaaa                                                                 >  1:856174/1‑140 (MQ=255)                                                                      tGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGaaaa                                                                <  2:441491/140‑1 (MQ=255)                                                                      tGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGaaaa                                                                >  1:495172/1‑140 (MQ=255)                                                                        ttGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCTCCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAAcc                                                              <  2:560663/140‑1 (MQ=255)                                                                        ttGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAAcc                                                              <  1:188101/140‑1 (MQ=255)                                                                        ttGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAAcc                                                              <  1:388023/140‑1 (MQ=255)                                                                          gcgcCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGACGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAAcccc                                                            <  1:585538/140‑1 (MQ=255)                                                                                gCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAACCCCGTATCa                                                      >  1:772833/1‑140 (MQ=255)                                                                                gCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAACCCCGTATCa                                                      >  1:241293/1‑140 (MQ=255)                                                                                  tCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAACCCCGTATCAGc                                                    <  2:946124/140‑1 (MQ=255)                                                                                         gCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAACCCCGTATCAGCGTGAGCa                                             <  1:102775/140‑1 (MQ=255)                                                                                          cGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAACCCCGTATCAGCGTGAGCag                                            <  1:586736/140‑1 (MQ=255)                                                                                           gTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAACCCCGTATCAGCGTGAGCaga                                           <  2:169117/140‑1 (MQ=255)                                                                                            tGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAACCCCGTATCAGCGTGAGCagag                                          >  1:971152/1‑140 (MQ=255)                                                                                              cgcgGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAACCCCGTATCAGCGTGAGCAGAGCt                                        >  1:122663/1‑140 (MQ=255)                                                                                                      ttGCTCCAGCAGATCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAACCCCGTATCAGCGTGAGCAGAGCTTCGTAATc                                <  1:897706/140‑1 (MQ=255)                                                                                                                  aTCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAACCCCGTATCAGCGTGAGCAGAGCTTCGTAATCGGCTTTTGAAAt                    >  2:1006498/1‑140 (MQ=255)                                                                                                                   tCGACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAACCCCGTATCAGCGTGAGCAGAGCTTCGTAATCGGCTTTTGAAATc                   >  2:474182/1‑140 (MQ=255)                                                                                                                     gACAATCTTTAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAACCCCGTATCAGCGTGAGCAGAGCTTCGTAATCGGCTTTTGAAATCAt                 <  2:500558/140‑1 (MQ=255)                                                                                                                              tAACTGGCGCTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAACCCCGTATCAGCGTGAACAGAGCTTTGTAATCGGCTTTTGAAATCATACCGCTGtt        >  2:822732/1‑140 (MQ=255)                                                                                                                                    gcgcTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAACCCCGTATCAGCGTGAGCAGAGCTTCGTAATCGGCTTTTGAAATCATACCGCTGTTGGCGTg  <  2:279400/140‑1 (MQ=255)                                                                                                                                    gcgcTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAACCCCGTATCAGCGTGAGCAGAGCTTCGTAATCGGCTTTTGAAATCATACCGCTGTTGGCGTg  <  2:241293/140‑1 (MQ=255)                                                                                                                                    gcgcTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAACCCCGTATCAGCGTGAGCAGAGCTTCGTAATCGGCTTTTGAAATCATACCGCTGTTGGCGTg  <  2:656208/140‑1 (MQ=255)                                                                                                                                    gcgcTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAACCCCGTATCAGCGTGAGCAGAGCTTCGTAATCGGCTTTTGAAATCATACCGCTGTTGGCGTg  <  1:541344/140‑1 (MQ=255)                                                                                                                                    gcgcTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAACCCCGTATCAGCGTGAGCAGAGCTTCGTAATCGGCTTTTGAAATCATACCGCTGTTGGCGTg  <  1:103470/140‑1 (MQ=255)                                                                                                                                    gcgcTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAACCCCGTATCAGCGTGAGCAGAGCTTCGTAATCGGCTTTTGAAATCATACCGCTGTTGGCGTg  <  1:1001074/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         GCTTTGCCGTTAAGGGTGAAGTTGAGATAGTCAATATCACGCAGGATGGTCCTGCCTGAAACGCCAGTCTGTTGCGCCAGCTCGCCAGGCGTGCGCGGCTGTTGCTCCAGCAGATCGACAATCTTTAACTGGCGTTCGTTTAGCATTAATCCACCTGACGGAACTGGCTAAACGCGTTGACTTTCTCCGCAGTTAAGGTCGTCAGAAAACCCCGTATCAGCGTGAGCAGAGCTTCGTAATCGGCTTTTGAAATCATACCGCTGTTGGCGTG  >  NC_000913/4089710‑4089980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑