Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,148,814	A→G	R484R (CGT→CGC)	eptC ←	phosphoethanolamine transferase EptC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,148,814	0	A	G	100.0%	236.0 / NA	63	R484R (CGT→CGC)	eptC	phosphoethanolamine transferase EptC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/24);  total (39/24)

GCCCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAAC  >  NC_000913/4148679‑4148948                                                                                                                                        |                                                                                                                                       gCCCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGcgcg                                                                                                                                    <  2:703315/140‑1 (MQ=255)    cGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCg                                                                                                                                 >  1:113302/1‑140 (MQ=255)    cGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCg                                                                                                                                 >  2:427191/1‑140 (MQ=255)     gCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCgg                                                                                                                                <  1:297831/140‑1 (MQ=255)      ccAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCggg                                                                                                                               <  1:623390/140‑1 (MQ=255)       cAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGt                                                                                                                              >  1:784362/1‑140 (MQ=255)            cTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTcc                                                                                                                         >  1:51054/1‑140 (MQ=255)                    gtgtGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACg                                                                                                                 <  2:361043/140‑1 (MQ=255)                         gATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCt                                                                                                            <  2:440435/140‑1 (MQ=255)                         gATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCt                                                                                                            >  2:310602/1‑140 (MQ=255)                              gTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGtt                                                                                                       >  1:795961/1‑140 (MQ=255)                              gTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGtt                                                                                                       >  1:853687/1‑140 (MQ=255)                              gTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGtt                                                                                                       >  1:767876/1‑140 (MQ=255)                              gTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGtt                                                                                                       >  1:565539/1‑140 (MQ=255)                              gTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGtt                                                                                                       >  1:118324/1‑140 (MQ=255)                              gTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGtt                                                                                                       >  1:341427/1‑140 (MQ=255)                              gTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGtt                                                                                                       >  1:199096/1‑140 (MQ=255)                              gTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGtt                                                                                                       >  1:326622/1‑140 (MQ=255)                               ttCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGttt                                                                                                      >  1:920757/1‑140 (MQ=255)                               ttCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGttt                                                                                                      >  1:769915/1‑140 (MQ=255)                               ttCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGttt                                                                                                      >  2:961806/1‑140 (MQ=255)                                   gCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTtgtg                                                                                                  <  1:664335/140‑1 (MQ=255)                                    ccAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTgc                                                                                                 >  1:196417/1‑140 (MQ=255)                                       ggCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTgcggc                                                                                              >  2:701289/1‑140 (MQ=255)                                        gCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTgcggcg                                                                                             >  2:195561/1‑140 (MQ=255)                                          tGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTgcggcggc                                                                                           <  1:329858/140‑1 (MQ=255)                                          tGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTgcggcggc                                                                                           <  1:210315/140‑1 (MQ=255)                                                 aCGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGt                                                                                    >  2:362555/1‑140 (MQ=255)                                                    aTCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTaaa                                                                                 <  2:683318/140‑1 (MQ=255)                                                    aTCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTaaa                                                                                 <  1:426081/140‑1 (MQ=255)                                                      cAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAAcc                                                                               >  2:292742/1‑140 (MQ=255)                                                      cAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAAcc                                                                               >  2:4727/1‑140 (MQ=255)                                                      cAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAAcc                                                                               >  1:1018857/1‑140 (MQ=255)                                                      cAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAAcc                                                                               >  1:503085/1‑140 (MQ=255)                                                       aaCATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACct                                                                              >  1:629591/1‑140 (MQ=255)                                                        aCATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACctc                                                                             >  2:735707/1‑140 (MQ=255)                                                        aCATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACctc                                                                             >  1:284665/1‑140 (MQ=255)                                                         cATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACctct                                                                            <  1:658744/140‑1 (MQ=255)                                                              tGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCAcc                                                                       >  1:479122/1‑140 (MQ=255)                                                               gCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCg                                                                      >  2:165175/1‑140 (MQ=255)                                                               gCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCg                                                                      >  2:801417/1‑140 (MQ=255)                                                                cGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGt                                                                     >  1:610321/1‑140 (MQ=255)                                                                    aaaTCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTc                                                                 >  2:731981/1‑140 (MQ=255)                                                                    aaaTCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTc                                                                 >  1:23638/1‑140 (MQ=255)                                                                           ggggATGAGTCGCTTGCCATTTTTCTGACGTCCTCAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAATCCTCTTCACCGTGGTCAGAGaaa                                                          <  2:156305/140‑1 (MQ=255)                                                                           ggggATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGaaa                                                          <  1:659464/140‑1 (MQ=255)                                                                              gATGAGTCGCTTGCCATTTTTCTGAAGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCTTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGCGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATaa                                                       >  2:995751/1‑140 (MQ=255)                                                                                   gTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAg                                                  >  2:974732/1‑140 (MQ=255)                                                                                   gTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAg                                                  >  2:569810/1‑140 (MQ=255)                                                                                     cGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGa                                                <  2:853687/140‑1 (MQ=255)                                                                                       cTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGaaa                                              <  2:379361/140‑1 (MQ=255)                                                                                         tGCCATTTTTCTTACGGCCACAGCAGGAAAGGGATGGGGTACATATGGCGCGGCGGGGTGTCCTCATTACGCCCCCGGGTTTTTTGCGGGGGCGGGTCGGAAAACTCTTCACCGTGGTGAGAGAAATAAACCAGGaaaac                                            >  2:857945/1‑138 (MQ=255)                                                                                                    tGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCGTTCGGGTTtg                                 <  2:920757/140‑1 (MQ=255)                                                                                                      aCGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCGTTCGGGTTtgct                               <  1:950628/140‑1 (MQ=255)                                                                                                             cagcagGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTaa                        <  1:180648/140‑1 (MQ=255)                                                                                                             cagcagGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTaa                        <  2:610321/140‑1 (MQ=255)                                                                                                             cagcagGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTaa                        >  1:223561/1‑140 (MQ=255)                                                                                                                     aCGGAATGGTGTACATATGGCGCGTCGGGGTGTCCTCATTACGCCCCTGAGGTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGCAATAAACCAGGCAACCGTTCGGGTCTGCCGCTTTAAAGTCTTTa                >  2:361910/1‑140 (MQ=255)                                                                                                                       ggAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAAt              <  1:790805/140‑1 (MQ=255)                                                                                                                              tgtACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTg       <  2:74892/140‑1 (MQ=255)                                                                                                                                tACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGc     <  2:429103/140‑1 (MQ=255)                                                                                                                                 aCATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCa    <  1:735707/140‑1 (MQ=255)                                                                                                                                 aCATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCa    >  1:444189/1‑140 (MQ=255)                                                                                                                                  cATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCaa   <  2:503085/140‑1 (MQ=255)                                                                                                                                   atatGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAAc  >  1:757349/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       GCCCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCGTTCGGGTTTGCTGCTTTAAAGTCTTTAATCAGGCTGGCAAC  >  NC_000913/4148679‑4148948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑