Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,326,444	A→G	G97G (GGT→GGC)	yjdM ←	conserved protein YjdM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,326,444	0	A	G	100.0%	193.4 / NA	51	G97G (GGT→GGC)	yjdM	conserved protein YjdM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/21);  total (30/21)

ATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAACCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAGATCTTTAATGATGGTAACGCTGTCGCCGTCAGCCA  >  NC_000913/4326311‑4326580                                                                                                                                      |                                                                                                                                         aTAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCg                                                                                                                                    >  1:143438/1‑140 (MQ=255)       cgcgCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTg                                                                                                                              >  1:514449/1‑140 (MQ=255)           cAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAAt                                                                                                                          >  2:807471/1‑140 (MQ=255)               cGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGAt                                                                                                                      >  1:281054/1‑140 (MQ=255)                gTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATg                                                                                                                     <  1:396994/140‑1 (MQ=255)                       cAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGt                                                                                                              <  1:398743/140‑1 (MQ=255)                           cATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGcc                                                                                                          <  2:87556/140‑1 (MQ=255)                           cATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGcc                                                                                                          <  1:898669/140‑1 (MQ=255)                                 gCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAAc                                                                                                    >  1:870763/1‑140 (MQ=255)                                 gCACCGTTGCCGGATGCGGCGCGAGCACCTTATCCGGCTTACCGATGACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAAc                                                                                                    >  2:832878/1‑140 (MQ=255)                                    ccGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAg                                                                                                 >  1:227401/1‑140 (MQ=255)                                    ccGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAg                                                                                                 >  1:58092/1‑140 (MQ=255)                                       ttGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCg                                                                                              >  1:925533/1‑140 (MQ=255)                                       ttGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCg                                                                                              >  1:905716/1‑140 (MQ=255)                                       ttGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCg                                                                                              >  2:764582/1‑140 (MQ=255)                                        tGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGCTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCgg                                                                                             >  1:777526/1‑140 (MQ=255)                                               tGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTACACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGtttt                                                                                      >  1:603813/1‑140 (MQ=255)                                                    cgcgAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACt                                                                                 >  2:378762/1‑140 (MQ=255)                                                             cTTATCCGGCTTACAGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCTACGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTTGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCa                                                                        <  2:908813/140‑1 (MQ=255)                                                                    ggCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCa                                                                 <  2:744772/140‑1 (MQ=255)                                                                    ggCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCa                                                                 <  2:143438/140‑1 (MQ=255)                                                                       ttACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCa                                                              >  2:143892/1‑140 (MQ=255)                                                                         aCCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATc                                                            >  2:838537/1‑140 (MQ=255)                                                                         aCCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATc                                                            >  1:346492/1‑140 (MQ=255)                                                                            gATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGaa                                                         >  2:448082/1‑140 (MQ=255)                                                                                 caatacaatCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCt                                                    <  2:386551/140‑1 (MQ=255)                                                                                    tacaatCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTAcc                                                 >  2:556257/1‑140 (MQ=255)                                                                                    tacaatCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTAcc                                                 >  1:100943/1‑140 (MQ=255)                                                                                    tacaatCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTAcc                                                 >  1:697764/1‑140 (MQ=255)                                                                                       aatCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCttt                                              <  1:828027/140‑1 (MQ=255)                                                                                         tCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCa                                            >  1:883069/1‑140 (MQ=255)                                                                                         tCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCa                                            >  1:309140/1‑140 (MQ=255)                                                                                           aGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCAcc                                          <  1:838537/140‑1 (MQ=255)                                                                                            gTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCt                                         >  1:1009131/1‑140 (MQ=255)                                                                                            gTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCt                                         >  1:494192/1‑140 (MQ=255)                                                                                            gTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCt                                         >  2:207539/1‑140 (MQ=255)                                                                                            gTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCt                                         >  1:594220/1‑140 (MQ=255)                                                                                            gTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCt                                         >  2:182965/1‑140 (MQ=255)                                                                                             ttCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCtt                                        <  2:227401/140‑1 (MQ=255)                                                                                              tCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTc                                       <  1:764582/140‑1 (MQ=255)                                                                                               cTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCa                                      <  2:66899/140‑1 (MQ=255)                                                                                               cTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCa                                      <  2:925533/140‑1 (MQ=255)                                                                                               cTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCa                                      <  2:514449/140‑1 (MQ=255)                                                                                                ttttaCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAg                                     <  2:603813/140‑1 (MQ=255)                                                                                                    tCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAGATCt                                 <  1:832878/140‑1 (MQ=255)                                                                                                         aaCTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAGATCTTTAat                            <  1:532889/140‑1 (MQ=255)                                                                                                               gATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAGATCTTTAATGATGGt                      <  2:281054/140‑1 (MQ=255)                                                                                                                   tCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAGATCTTTAATGATGGTAACg                  <  2:346492/140‑1 (MQ=255)                                                                                                                        ttCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAGATCTTTAATGATGGTAACGCTGTc             >  1:455989/1‑140 (MQ=255)                                                                                                                          cATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAGATCTTTAATGATGGTAACGCTGTCGc           >  1:460031/1‑140 (MQ=255)                                                                                                                                 ccAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAGATCTTTAATGATGGTAACGCTGTCGCCGTcagc    <  2:178605/140‑1 (MQ=255)                                                                                                                                   aaagCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAGATCTTTAATGATGGTAACGCTGTCGCCGTcagcca  <  2:870763/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         ATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAACCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAGATCTTTAATGATGGTAACGCTGTCGCCGTCAGCCA  >  NC_000913/4326311‑4326580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑