Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,469,825	A→G	N734S (AAC→AGC)	mgtA →	Mg(2(+)) importing P‑type ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,469,825	0	A	G	100.0%	320.9 / NA	84	N734S (AAC→AGC)	mgtA	Mg(2(+)) importing P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/42);  total (42/42)

CGTCGCACTTTCGCCAACATGCTGAAATACATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTC  >  NC_000913/4469689‑4469961                                                                                                                                         |                                                                                                                                         cGTCGCACTTTCGCCAACATGCTGAAAGACATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCct                                                                                                                                       <  2:998478/140‑1 (MQ=255)   tCGCACTTTCGCCAACATGCTGAAATACATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCctgc                                                                                                                                     >  2:670653/1‑140 (MQ=255)    cGCACTTTCGCCAACATGCTGAAATACATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCctgct                                                                                                                                    >  2:91521/1‑140 (MQ=255)    cGCACTTTCGCCAACATGCTGAAATACATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCctgct                                                                                                                                    >  2:492371/1‑140 (MQ=255)       aCTTTCGCCAACATGCTGAAATACATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTa                                                                                                                                 <  2:388872/140‑1 (MQ=255)         tttCGCCAACATGCTGAAATACATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACg                                                                                                                               <  1:442771/140‑1 (MQ=255)           tCGCCAACATGCTGAAATACATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGAt                                                                                                                             <  2:364432/140‑1 (MQ=255)            cGCCAACATGCTGAAATACATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGAtg                                                                                                                            >  2:504604/1‑140 (MQ=255)                 aCATGCTGAAATACATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTca                                                                                                                       <  2:718020/140‑1 (MQ=255)                     gCTGAAATACATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAgg                                                                                                                   >  2:247143/1‑140 (MQ=255)                       tGAAATACATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTg                                                                                                                 <  2:63287/140‑1 (MQ=255)                       tGAAATACATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTg                                                                                                                 >  1:390832/1‑140 (MQ=255)                       tGAAATACATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTGGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTg                                                                                                                 <  2:1024956/140‑1 (MQ=255)                           aTACATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGa                                                                                                             <  2:623540/140‑1 (MQ=255)                            tACATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGAt                                                                                                            <  2:2800/140‑1 (MQ=255)                             aCATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATc                                                                                                           <  2:677677/140‑1 (MQ=255)                             aCATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATc                                                                                                           <  2:852252/140‑1 (MQ=255)                             aCATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATc                                                                                                           >  2:104488/1‑140 (MQ=255)                              cATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATcc                                                                                                          >  1:313601/1‑140 (MQ=255)                              cATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATcc                                                                                                          >  2:519390/1‑140 (MQ=255)                               aTCAAAATGATGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATccc                                                                                                         >  2:236928/1‑140 (MQ=255)                               aTCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATccc                                                                                                         >  2:10420/1‑140 (MQ=255)                               aTCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATccc                                                                                                         >  2:869933/1‑140 (MQ=255)                                tCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCg                                                                                                        <  1:485507/140‑1 (MQ=255)                                      tGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGAt                                                                                                  <  2:129183/140‑1 (MQ=255)                                         cGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAAc                                                                                               >  2:610129/1‑140 (MQ=255)                                         cGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAAc                                                                                               >  1:784068/1‑140 (MQ=255)                                                         cGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCaaa                                                                               <  1:519390/140‑1 (MQ=255)                                                          ggTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAAt                                                                              >  1:1001608/1‑140 (MQ=255)                                                          ggTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAAt                                                                              >  1:357540/1‑140 (MQ=255)                                                          ggTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAAt                                                                              >  1:679361/1‑140 (MQ=255)                                                               tgtgTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCaaa                                                                         >  2:423848/1‑140 (MQ=255)                                                               tgtgTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCaaa                                                                         >  1:808249/1‑140 (MQ=255)                                                                   ttCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCAGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGcc                                                                     >  1:946098/1‑140 (MQ=255)                                                                    tCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCg                                                                    <  2:28502/140‑1 (MQ=255)                                                                    tCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCg                                                                    <  1:1031594/140‑1 (MQ=255)                                                                     cAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGc                                                                   <  1:716642/140‑1 (MQ=255)                                                                     cAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGc                                                                   <  1:385115/140‑1 (MQ=255)                                                                             tGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTgg                                                           >  1:491737/1‑140 (MQ=255)                                                                             tGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACAACGAGCAAATTCAAAAGCCGCAGCGTTgg                                                           >  2:34665/1‑140 (MQ=255)                                                                              ggTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGa                                                          >  2:479998/1‑140 (MQ=255)                                                                                     aGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCcggc                                                   >  2:237702/1‑140 (MQ=255)                                                                                     aGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCcggc                                                   >  1:985406/1‑140 (MQ=255)                                                                                         cTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGAt                                               >  1:1015467/1‑140 (MQ=255)                                                                                              ttGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTggg                                          <  1:104488/140‑1 (MQ=255)                                                                                               tGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTgggg                                         <  1:342716/140‑1 (MQ=255)                                                                                               tGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTgggg                                         <  1:456900/140‑1 (MQ=255)                                                                                               tGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTgggg                                         <  2:697126/140‑1 (MQ=255)                                                                                               tGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTgggg                                         <  1:843653/140‑1 (MQ=255)                                                                                                gCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGATGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGgc                                        <  1:124415/140‑1 (MQ=255)                                                                                                gCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGgc                                        <  2:920665/140‑1 (MQ=255)                                                                                                 ccctccctGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGTCGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGgcg                                       <  2:1626/140‑1 (MQ=255)                                                                                                 cccTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTTGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGgcg                                       >  1:908355/1‑140 (MQ=255)                                                                                                 cccTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGgcg                                       >  2:723284/1‑140 (MQ=255)                                                                                                 cccTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGgcg                                       >  2:393213/1‑140 (MQ=255)                                                                                                 cccTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGgcg                                       >  2:257309/1‑140 (MQ=255)                                                                                                  ccTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGgcgc                                      <  1:650879/140‑1 (MQ=255)                                                                                                   cTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCt                                     <  1:610129/140‑1 (MQ=255)                                                                                                     tCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCttt                                   >  1:438621/1‑140 (MQ=255)                                                                                                      ccTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTa                                  >  1:202206/1‑140 (MQ=255)                                                                                                      ccTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTa                                  <  1:257309/140‑1 (MQ=255)                                                                                                      ccTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTa                                  >  1:690435/1‑140 (MQ=255)                                                                                                      ccTGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTa                                  <  2:784068/140‑1 (MQ=255)                                                                                                        tGCCGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATg                                >  2:72880/1‑140 (MQ=255)                                                                                                          ccGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGAt                              <  1:492371/140‑1 (MQ=255)                                                                                                          ccGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGAt                              <  2:752349/140‑1 (MQ=255)                                                                                                          ccGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGAt                              <  1:342927/140‑1 (MQ=255)                                                                                                          ccGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGAt                              <  2:491737/140‑1 (MQ=255)                                                                                                          ccGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGAt                              <  1:670653/140‑1 (MQ=255)                                                                                                          ccGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGAt                              <  1:91521/140‑1 (MQ=255)                                                                                                          ccGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGAt                              <  2:383225/140‑1 (MQ=255)                                                                                                           cGATGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGAtc                             >  2:156188/1‑140 (MQ=255)                                                                                                              tGTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGAtcttc                          >  1:383342/1‑140 (MQ=255)                                                                                                               gTTGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCCCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGAtcttct                         >  1:548103/1‑140 (MQ=255)                                                                                                                ttGCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATATGGGGCGCTTTATGATcttctt                        >  1:440898/1‑140 (MQ=255)                                                                                                                  gCCGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCg                      >  2:13307/1‑140 (MQ=255)                                                                                                                    cGTTACACTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGa                    <  1:754495/140‑1 (MQ=255)                                                                                                                         cacTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGAt               <  2:880498/140‑1 (MQ=255)                                                                                                                         cacTTGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGAt               <  2:214294/140‑1 (MQ=255)                                                                                                                            ttGCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAg            >  2:377558/1‑140 (MQ=255)                                                                                                                              gCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCt          >  1:558599/1‑140 (MQ=255)                                                                                                                              gCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCt          <  1:746282/140‑1 (MQ=255)                                                                                                                              gCTTATTCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCt          <  1:895184/140‑1 (MQ=255)                                                                                                                                   ttCAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATc     >  2:899916/1‑140 (MQ=255)                                                                                                                                     cAGAGCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCCCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCtt   <  2:548103/140‑1 (MQ=255)                                                                                                                                      agagCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTc  <  1:247143/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CGTCGCACTTTCGCCAACATGCTGAAATACATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTC  >  NC_000913/4469689‑4469961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑