| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,472,714 | T→C | intergenic (‑181/+100) | pyrL ← / ← yjgH | pyrBI operon leader peptide/RutC family protein YjgH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,472,714 | 0 | T | C | 98.1% | 197.5 / ‑3.9 | 53 | intergenic (‑181/+100) | pyrL/yjgH | pyrBI operon leader peptide/RutC family protein YjgH |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (22/30); minor base A (0/1); total (22/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCTGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTACTGCTCAGGGATGCGCGCTATCACTT > NC_000913/4472581‑4472842 | gcgcAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGctttt < 2:531949/140‑1 (MQ=255) cAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGctttttcc < 2:497259/140‑1 (MQ=255) cAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGctttttcc > 1:381542/1‑140 (MQ=255) aGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGctttttcctt > 2:85390/1‑140 (MQ=255) aaTATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTtttcactttcac < 1:671380/140‑1 (MQ=255) atatTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACg > 1:759559/1‑140 (MQ=255) atTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCa < 1:113335/140‑1 (MQ=255) gTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAg < 1:1037865/140‑1 (MQ=255) gTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAg < 1:468341/140‑1 (MQ=255) cAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGat > 1:345536/1‑140 (MQ=255) gcgcAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGatat < 2:644593/140‑1 (MQ=255) gcgcAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGatat < 2:218556/140‑1 (MQ=255) gcgcAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGatat > 1:502114/1‑140 (MQ=255) cgcAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATg > 1:881357/1‑140 (MQ=255) aaTTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCAt < 1:51363/140‑1 (MQ=255) aaTTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCAt < 1:216182/140‑1 (MQ=255) aaTTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCAGCTTTTTCCTTTTTCTCTTTCACGCAATCAGATATGCATTTTATTCCATTCAt < 2:586258/140‑1 (MQ=255) ttAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATcc < 1:99592/140‑1 (MQ=255) tAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCg < 1:172652/140‑1 (MQ=255) tCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTtat < 2:898440/140‑1 (MQ=255) ggTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAggg < 1:116875/140‑1 (MQ=255) tataTGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCg < 1:733958/140‑1 (MQ=255) tataTGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCg < 2:827900/140‑1 (MQ=255) tataTGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCg < 1:1027681/140‑1 (MQ=255) tataTGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCg < 1:30974/140‑1 (MQ=255) tttttATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGa > 1:651130/1‑140 (MQ=255) ttttATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGaa > 2:618147/1‑140 (MQ=255) ttttATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGaa > 2:608053/1‑140 (MQ=255) tttATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTTTAGGGCGGAGTGTGaaa > 2:364937/1‑140 (MQ=255) tATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGTGGAGTGTGAAAGc < 1:1434/140‑1 (MQ=255) tATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGc > 1:881868/1‑140 (MQ=255) aTTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCg < 2:502114/140‑1 (MQ=255) tttGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGa < 1:334659/140‑1 (MQ=255) tttGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGa < 2:46729/140‑1 (MQ=255) aTGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACgg > 1:360434/1‑140 (MQ=255) tgtgATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCGCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGc > 1:238621/1‑140 (MQ=255) gtgATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCt < 2:273266/140‑1 (MQ=255) gATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTaa > 1:67765/1‑140 (MQ=255) gATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTaa > 1:113296/1‑140 (MQ=255) ttCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAaca < 1:85390/140‑1 (MQ=255) gAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTa < 2:381542/140‑1 (MQ=255) gAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTa < 2:113296/140‑1 (MQ=255) gAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTa < 2:881868/140‑1 (MQ=255) gAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTa < 2:601396/140‑1 (MQ=255) gAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTa < 2:67765/140‑1 (MQ=255) cTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTACTg > 2:374005/1‑140 (MQ=255) gTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTACTGCt > 1:1038658/1‑140 (MQ=255) cGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTACTGCTCAGGGa > 2:43334/1‑140 (MQ=255) aataatTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTACTGCTCAGGGATgcgcgc > 2:558077/1‑140 (MQ=255) aatTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGCAGTGTGAAAGCGAACGGCTAACACTATTGCTTACTGCTCAGGGATGCGCGCTAt < 2:759559/140‑1 (MQ=255) ttGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTACTGCTCAGGGATGCGCGCTATCa > 2:861061/1‑140 (MQ=255) tGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTACTGCTCAGGGATGCGCGCTATCAc > 1:724908/1‑140 (MQ=255) cAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTACTGCTCAGGGATGCGCGCTATCACtt > 1:782417/1‑140 (MQ=255) | GCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCTGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTACTGCTCAGGGATGCGCGCTATCACTT > NC_000913/4472581‑4472842 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |