| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,516,827 | C→T | A297A (GCG→GCA) | fecR ← | regulator for fec operon, periplasmic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,516,827 | 0 | C | T | 96.1% | 160.1 / ‑1.4 | 51 | A297A (GCG→GCA) | fecR | regulator for fec operon, periplasmic |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (34/15); total (35/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01 | |||||||||||
TTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGC > NC_000913/4516694‑4516959 | tttttGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATa < 1:673494/140‑1 (MQ=255) ttCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGAt < 1:422218/140‑1 (MQ=255) tGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCgg < 1:541592/140‑1 (MQ=255) aaCGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTAttttt > 2:623876/1‑140 (MQ=255) aaCGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTAttttt > 1:171159/1‑140 (MQ=255) tAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACg < 1:563159/140‑1 (MQ=255) aCAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTc > 1:213878/1‑140 (MQ=255) aCAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTc > 1:447315/1‑140 (MQ=255) aCAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTc < 1:869965/140‑1 (MQ=255) aCAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTc > 2:78082/1‑140 (MQ=255) aCAATCGAAATAAGAATTATTTTCCGTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTc > 1:862461/1‑140 (MQ=255) cAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCa > 2:933964/1‑140 (MQ=255) cGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGgcg < 2:95584/140‑1 (MQ=255) cGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGgcg < 2:503939/140‑1 (MQ=255) tCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAAcggcgg < 1:539918/140‑1 (MQ=255) aCAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATcg > 1:208272/1‑140 (MQ=255) gtgAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATcgcagcgc > 2:916677/1‑140 (MQ=255) gtgAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATcgcagcgc > 1:80391/1‑140 (MQ=255) tgAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATcgcagcgca > 2:309981/1‑140 (MQ=255) aaTGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCgcagca < 1:594254/140‑1 (MQ=255) gTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGc > 1:502530/1‑140 (MQ=255) tttATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAGCGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGcc > 2:758863/1‑140 (MQ=255) tttATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGcc > 2:21403/1‑140 (MQ=255) tttATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGcc > 1:702632/1‑140 (MQ=255) tttATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAACGCAGCAcccc > 2:744457/1‑140 (MQ=255) aCCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTTCGGTaa > 1:279353/1‑140 (MQ=255) aCCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTaa > 1:736305/1‑140 (MQ=255) aCCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCCCCGCACGCCGTGGCGGGaa > 2:210381/1‑140 (MQ=255) cgcgTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGACTGCAGCGGAGCACGACGTTGCggggaac > 2:726536/1‑135 (MQ=255) gcgTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACgg < 1:933964/140‑1 (MQ=255) cgTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACggg > 2:998612/1‑140 (MQ=255) cgTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACggg > 2:56046/1‑140 (MQ=255) cgTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACggg > 1:108822/1‑140 (MQ=255) cgTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACggg > 1:909309/1‑140 (MQ=255) cgTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACggg > 1:68799/1‑140 (MQ=255) cgTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACggg > 1:700366/1‑140 (MQ=255) cgTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGGAACggg > 2:799729/1‑140 (MQ=255) cgTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGGTGCGGTAACggg > 2:826733/1‑140 (MQ=255) cgTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGGTGCGGTAACggg > 2:782509/1‑140 (MQ=255) cgTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGGTGCGGTAACggg > 2:748641/1‑140 (MQ=255) cgTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGGTGCGGGAACggg > 2:343318/1‑140 (MQ=255) cgTAATAGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCCTTGCGGTAACggg > 1:611036/1‑140 (MQ=255) aGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCg < 1:799729/140‑1 (MQ=255) aGACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCg < 1:289623/140‑1 (MQ=255) gACTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGt < 1:202804/140‑1 (MQ=255) aCTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCGGCACGCCGTTGCGGTAAAGGGTTTGCGTg > 1:601722/1‑140 (MQ=255) aCTGAATTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTg > 1:858189/1‑140 (MQ=255) aaTTTTAACGGGAAGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTa < 1:826733/140‑1 (MQ=255) aaTTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTa < 2:171159/140‑1 (MQ=255) aaGCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGGTCGCCGCGCAGCACGCCGTTGCGGTAACGGGGTAACGTGGCTATcaccccacccc > 2:597456/1‑139 (MQ=255) gCGTTTGTGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGc < 1:722180/140‑1 (MQ=255) | TTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGC > NC_000913/4516694‑4516959 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |