Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,520,052	T→C	E91E (GAA→GAG)	yjhU ←	KpLE2 phage‑like element; putative DNA‑binding transcriptional regulator YjhU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,520,052	0	T	C	100.0%	295.7 / NA	78	E91E (GAA→GAG)	yjhU	KpLE2 phage‑like element; putative DNA‑binding transcriptional regulator YjhU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (51/27);  total (51/27)

TTGCTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCGGGCTGTTTGCA  >  NC_000913/4519915‑4520182                                                                                                                                          |                                                                                                                                   ttGCTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTc                                                                                                                                  >  2:35008/1‑140 (MQ=255)    cTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGcc                                                                                                                               >  1:522175/1‑140 (MQ=255)           gATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGc                                                                                                                        <  1:742045/140‑1 (MQ=255)             tGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCAc                                                                                                                      <  2:616724/140‑1 (MQ=255)             tGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCAc                                                                                                                      <  2:529726/140‑1 (MQ=255)             tGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCAc                                                                                                                      <  2:47408/140‑1 (MQ=255)               gATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACga                                                                                                                    <  2:538634/140‑1 (MQ=255)               gATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCGCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACga                                                                                                                    <  1:682376/140‑1 (MQ=255)                aTTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACgat                                                                                                                   <  2:726951/140‑1 (MQ=255)                  tGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACgatga                                                                                                                 <  1:276978/140‑1 (MQ=255)                    ttcttcCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATc                                                                                                               <  1:221073/140‑1 (MQ=255)                    ttcttcCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATc                                                                                                               >  1:8445/1‑140 (MQ=255)                       ttcCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCAct                                                                                                            <  1:619964/140‑1 (MQ=255)                         ccccAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCActct                                                                                                          >  2:696769/1‑140 (MQ=255)                            cAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTg                                                                                                       <  2:1001987/140‑1 (MQ=255)                              ggCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGga                                                                                                     >  2:897021/1‑140 (MQ=255)                                   cccccAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTg                                                                                                >  2:448084/1‑140 (MQ=255)                                   cccccAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTg                                                                                                >  2:936909/1‑140 (MQ=255)                                             aTCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATcgc                                                                                      >  1:285065/1‑140 (MQ=255)                                              tCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATcgcg                                                                                     >  2:429166/1‑140 (MQ=255)                                               cACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGa                                                                                    >  2:515341/1‑140 (MQ=255)                                               cACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGa                                                                                    <  2:782974/140‑1 (MQ=255)                                                         aaTCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAag                                                                          >  2:394692/1‑140 (MQ=255)                                                         aaTCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAag                                                                          >  2:256062/1‑140 (MQ=255)                                                          aTCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAagc                                                                         <  2:924115/140‑1 (MQ=255)                                                           tCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAagca                                                                        >  2:928297/1‑140 (MQ=255)                                                            cACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAagcag                                                                       >  2:61614/1‑140 (MQ=255)                                                            cACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAagcag                                                                       >  2:896292/1‑140 (MQ=255)                                                              ccTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCg                                                                     >  1:700677/1‑140 (MQ=255)                                                              ccTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCg                                                                     >  2:863484/1‑140 (MQ=255)                                                              ccTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCg                                                                     >  1:249526/1‑140 (MQ=255)                                                              ccTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTGGGGATCATCACGTTTTCCAGGCTGGTCGCCATGCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAAGTTGCAGAGCAACg                                                                     >  2:938878/1‑140 (MQ=255)                                                               cTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGt                                                                    >  2:330597/1‑140 (MQ=255)                                                                 gATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCg                                                                  >  1:883644/1‑140 (MQ=255)                                                                       gATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATagag                                                            >  2:329428/1‑140 (MQ=255)                                                                         tACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGcc                                                          >  2:77529/1‑140 (MQ=255)                                                                          aCATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCa                                                         <  2:307826/140‑1 (MQ=255)                                                                            aTGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGGATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCaaa                                                       >  1:933433/1‑140 (MQ=255)                                                                            aTGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGTGTCGATAGAGCCaaa                                                       >  2:987410/1‑140 (MQ=255)                                                                             tGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAg                                                      >  2:898687/1‑140 (MQ=255)                                                                             tGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAg                                                      >  2:951711/1‑140 (MQ=255)                                                                             tGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAg                                                      >  2:582653/1‑140 (MQ=255)                                                                             tGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAg                                                      >  2:808968/1‑140 (MQ=255)                                                                             tGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAg                                                      >  1:171097/1‑140 (MQ=255)                                                                             tGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAg                                                      >  1:194297/1‑140 (MQ=255)                                                                             tGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAg                                                      >  1:206854/1‑140 (MQ=255)                                                                              gCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGa                                                     >  2:760225/1‑140 (MQ=255)                                                                              gCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGa                                                     >  1:921434/1‑140 (MQ=255)                                                                              gCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGa                                                     >  1:994616/1‑140 (MQ=255)                                                                                  ccGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACAtt                                                 >  1:280668/1‑140 (MQ=255)                                                                                  ccGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACAtt                                                 <  1:429166/140‑1 (MQ=255)                                                                                  ccGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACAtt                                                 >  1:435551/1‑140 (MQ=255)                                                                                  ccGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACAtt                                                 <  1:515341/140‑1 (MQ=255)                                                                                  ccGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACAtt                                                 >  1:988670/1‑140 (MQ=255)                                                                                    ggCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTca                                               >  1:527842/1‑140 (MQ=255)                                                                                     gCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTcac                                              >  1:213984/1‑140 (MQ=255)                                                                                     gCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATGTGCAAAGCAGCGTCGACAGAGCCAAAGACATTcac                                              >  2:1005877/1‑140 (MQ=255)                                                                                        cGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATc                                           >  1:565175/1‑140 (MQ=255)                                                                                            aaaGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAgg                                       <  2:206854/140‑1 (MQ=255)                                                                                             aaGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGt                                      <  2:256333/140‑1 (MQ=255)                                                                                             aaGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGt                                      <  2:8749/140‑1 (MQ=255)                                                                                                 cGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGCTGat                                  >  1:1009318/1‑140 (MQ=255)                                                                                                   aGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGatat                                >  1:441096/1‑140 (MQ=255)                                                                                                     tCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGa                              >  1:770663/1‑140 (MQ=255)                                                                                                       cGCATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAAc                            >  2:119417/1‑140 (MQ=255)                                                                                                         cATCAGCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCa                          <  1:35008/140‑1 (MQ=255)                                                                                                              gCTTTGTATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCa                     <  1:472205/140‑1 (MQ=255)                                                                                                                    tATCATCACGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTcc               >  1:413671/1‑140 (MQ=255)                                                                                                                        atcaCGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCggg           <  2:987104/140‑1 (MQ=255)                                                                                                                        atcaCGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCggg           <  2:237201/140‑1 (MQ=255)                                                                                                                        atcaCGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCggg           <  1:928297/140‑1 (MQ=255)                                                                                                                         tcaCGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCGGGc          >  1:207604/1‑140 (MQ=255)                                                                                                                         tcaCGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCGGGc          >  2:115627/1‑140 (MQ=255)                                                                                                                          caCGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCGGGCt         <  2:204207/140‑1 (MQ=255)                                                                                                                            cGTTTTCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCGGGCTGt       >  2:718705/1‑140 (MQ=255)                                                                                                                              ttttCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCGGGCTGttt     >  1:514751/1‑140 (MQ=255)                                                                                                                              ttttCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCGGGCTGttt     >  2:336500/1‑140 (MQ=255)                                                                                                                                ttCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCGGGCTGTTTGc   <  1:897021/140‑1 (MQ=255)                                                                                                                                 tCCGGCATGCTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCGGGCTGTTTGCa  <  1:951711/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   TTGCTCATCTGATGGATTGTTCTTCCCCAGGCAACCCCCAGCACATCACCATTCTCAATCACCTGATTCAGATACATGCCACCGGCACGTGAAAGGCGAGTCCGCATCAGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCGGGCTGTTTGCA  >  NC_000913/4519915‑4520182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑