Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	372,774	A→G	E220E (GAA→GAG)	mhpD →	2‑hydroxypentadienoate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	372,774	0	A	G	100.0%	152.9 / NA	42	E220E (GAA→GAG)	mhpD	2‑hydroxypentadienoate hydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/18);  total (24/18)

GGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAG  >  NC_000913/372638‑372910                                                                                                                                         |                                                                                                                                         ggTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCg                                                                                                                                       >  2:594717/1‑140 (MQ=255)      gACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTgcg                                                                                                                                  <  2:482238/140‑1 (MQ=255)            aaaaaCTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGTCGCTGCGCACCgg                                                                                                                            <  2:258010/140‑1 (MQ=255)            aaaaaCTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCgg                                                                                                                            <  1:1032464/140‑1 (MQ=255)            aaaaaCTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCgg                                                                                                                            >  2:391074/1‑140 (MQ=255)            aaaaaCTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCgg                                                                                                                            <  1:590643/140‑1 (MQ=255)                 cTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGata                                                                                                                       >  2:766078/1‑140 (MQ=255)                 cTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGata                                                                                                                       <  2:262955/140‑1 (MQ=255)                 cTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGata                                                                                                                       <  1:825600/140‑1 (MQ=255)                                  cgcgTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCa                                                                                                      <  1:766078/140‑1 (MQ=255)                                   gcgTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCAt                                                                                                     >  2:340617/1‑140 (MQ=255)                                      taataaCGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAg                                                                                                  >  2:927827/1‑140 (MQ=255)                                              aaGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGAtg                                                                                          >  1:114958/1‑140 (MQ=255)                                               agagGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGAtgg                                                                                         >  1:47071/1‑140 (MQ=255)                                                   gTTTCTAGCGGGCGCGGCAGCGAATGCCTGGCACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCAtt                                                                                                    <  2:612955/125‑1 (MQ=255)                                                   gTTTCTAGCGGGCGCGGCAGCGAATGCCTGGCACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCAtt                                                                                                    >  1:612955/1‑125 (MQ=255)                                                      tCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCggg                                                                                  >  2:1038069/1‑139 (MQ=255)                                                      tCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGt                                                                                  >  2:895660/1‑140 (MQ=255)                                                             ggCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCg                                                                           >  2:142887/1‑140 (MQ=255)                                                              gcgcGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCgg                                                                          >  1:180556/1‑140 (MQ=255)                                                              gcgcGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCACTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCgg                                                                          >  1:472920/1‑140 (MQ=255)                                                               cgcgGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCggg                                                                         >  2:591715/1‑140 (MQ=255)                                                                   gCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGAt                                                                     <  1:594717/140‑1 (MQ=255)                                                                    cAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATc                                                                    >  1:164458/1‑140 (MQ=255)                                                                         aaTGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGtttt                                                               <  1:676881/140‑1 (MQ=255)                                                                           tGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGa                                                             <  1:757784/140‑1 (MQ=255)                                                                           tGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGa                                                             <  1:60728/140‑1 (MQ=255)                                                                                 ggACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCa                                                       >  2:670643/1‑140 (MQ=255)                                                                                 ggACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCa                                                       >  2:653668/1‑140 (MQ=255)                                                                                     aTCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATAtt                                                   >  1:240324/1‑140 (MQ=255)                                                                                       ccGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGa                                                 >  1:842847/1‑140 (MQ=255)                                                                                       ccGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGa                                                 >  1:638127/1‑140 (MQ=255)                                                                                        cGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGaa                                                >  1:494580/1‑140 (MQ=255)                                                                                              aTGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATa                                          <  1:417327/140‑1 (MQ=255)                                                                                               tGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGCGTGAGCCGCTGCGCACCGGGGATATCATCCTCACCGGGGCATTCGGAGCGATGGCGGCGGTGAATGCGGGCGATCGTTTTGCAGCCCATATTGAACGCCTAg                                         >  2:65238/1‑140 (MQ=255)                                                                                                                cACGCAAAATGGCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACa                        >  2:918844/1‑140 (MQ=255)                                                                                                                          ggCCAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAgcg              <  2:180556/140‑1 (MQ=255)                                                                                                                            ccAGTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCa            <  1:1038069/140‑1 (MQ=255)                                                                                                                               gTCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGcc         <  2:114958/140‑1 (MQ=255)                                                                                                                                tCTGGGTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGccc        >  1:340175/1‑140 (MQ=255)                                                                                                                                     gTGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCaaaa   >  1:682291/1‑140 (MQ=255)                                                                                                                                      tGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAg  <  1:340617/140‑1 (MQ=255)                                                                                                                                      tGAGCCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAg  <  1:391074/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAG  >  NC_000913/372638‑372910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑