Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	388,666	C→T	intergenic (+20/+87)	tauD → / ← hemB	taurine dioxygenase/porphobilinogen synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	388,666	0	C	T	100.0%	191.0 / NA	55	intergenic (+20/+87)	tauD/hemB	taurine dioxygenase/porphobilinogen synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/29);  total (26/29)

GCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAATCTTCTTCTCAGCCAAATCCAGCGCAAAGT  >  NC_000913/388530‑388793                                                                                                                                         |                                                                                                                                gCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCg                                                                                                                              <  1:509941/140‑1 (MQ=255) gCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCg                                                                                                                              <  2:762088/140‑1 (MQ=255) gCGATTTGAGATAACCGCGAGACCCAGCACTATGCCATTGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGGCGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCg                                                                                                                              <  2:338494/140‑1 (MQ=255)         ggATAACCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTg                                                                                                                      <  1:955657/140‑1 (MQ=255)         ggATAACCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTg                                                                                                                      <  1:602836/140‑1 (MQ=255)             aaCCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGga                                                                                                                  <  1:302640/140‑1 (MQ=255)             aaCCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGga                                                                                                                  <  1:35992/140‑1 (MQ=255)             aaCCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGga                                                                                                                  <  1:390396/140‑1 (MQ=255)             aaCCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGga                                                                                                                  <  1:459204/140‑1 (MQ=255)             aaCCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGga                                                                                                                  <  2:990126/140‑1 (MQ=255)             aaCCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGga                                                                                                                  <  2:638730/140‑1 (MQ=255)             aaCCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGga                                                                                                                  <  1:754754/140‑1 (MQ=255)               ccGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGgaga                                                                                                                <  2:723862/140‑1 (MQ=255)                    tGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGtt                                                                                                           >  2:145214/1‑140 (MQ=255)                          aGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTg                                                                                                     <  1:176236/140‑1 (MQ=255)                               tATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAgggg                                                                                                >  1:858206/1‑140 (MQ=255)                                 tGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGc                                                                                              >  1:638543/1‑140 (MQ=255)                                 tGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGc                                                                                              >  2:908716/1‑140 (MQ=255)                                 tGCCAATGCCGATTACCTGCCACAGCGACGGATAATACATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGATAGGGTGAGGGGGc                                                                                              >  2:389509/1‑140 (MQ=255)                                     aaTGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTc                                                                                          <  2:930867/140‑1 (MQ=255)                                      aTGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCa                                                                                         >  2:361172/1‑140 (MQ=255)                                      aTGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCa                                                                                         <  1:967000/140‑1 (MQ=255)                                        gCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCAcc                                                                                       <  1:642508/140‑1 (MQ=255)                                          cGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGt                                                                                     <  1:100153/140‑1 (MQ=255)                                           gATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTa                                                                                    >  2:425607/1‑140 (MQ=255)                                                   gCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAAc                                                                            >  1:679971/1‑140 (MQ=255)                                                      acaGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAgg                                                                         <  2:1030443/140‑1 (MQ=255)                                                          cGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTaa                                                                     <  2:920759/140‑1 (MQ=255)                                                          cGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTaa                                                                     <  2:323740/140‑1 (MQ=255)                                                          cGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTaa                                                                     <  1:908716/140‑1 (MQ=255)                                                            aCGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACt                                                                   <  1:861592/140‑1 (MQ=255)                                                              ggATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTcc                                                                 <  2:858206/140‑1 (MQ=255)                                                                       aTCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTg                                                        >  2:483379/1‑140 (MQ=255)                                                                         cGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTgag                                                      >  1:792291/1‑140 (MQ=255)                                                                           ggCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGagag                                                    >  2:143790/1‑140 (MQ=255)                                                                           ggCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGagag                                                    <  1:44790/140‑1 (MQ=255)                                                                           ggCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGagag                                                    >  2:478797/1‑140 (MQ=255)                                                                                             tAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAg                                  >  1:411597/1‑140 (MQ=255)                                                                                             tAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAg                                  >  2:427369/1‑140 (MQ=255)                                                                                             tAAACCGTTTTATCGGGCGGGGTAATACGAGAGAGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAg                                  >  1:16606/1‑140 (MQ=255)                                                                                              aaaCCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGa                                 >  1:151779/1‑140 (MQ=255)                                                                                                  cGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGG‑TAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAAtctt                            >  2:144715/1‑140 (MQ=255)                                                                                                   gTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAAtctt                            >  2:479216/1‑140 (MQ=255)                                                                                                      ttATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAAtcttctt                         >  1:193355/1‑140 (MQ=255)                                                                                                       tATCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGG‑TAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAAtcttcttct                       <  1:144715/140‑1 (MQ=255)                                                                                                        aTCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAAtcttcttct                       <  2:429314/140‑1 (MQ=255)                                                                                                        aTCGGGCGGGGTAATACGAGAGTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAAtcttcttct                       <  2:116023/140‑1 (MQ=255)                                                                                                                           gagTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAATCTTCTTCTCAGTCAAATCCAGCGCaaa    >  2:126847/1‑140 (MQ=255)                                                                                                                           gagTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAATCTTCTTCTCAGCCAAATCCAGCGCaaa    >  2:529677/1‑140 (MQ=255)                                                                                                                           gagTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAATCTTCTTCTCAGCCAAATCCAGCGCaaa    >  2:975841/1‑140 (MQ=255)                                                                                                                            agTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAATCTTCTTCTCAGCCAAATCCAGCGCAAAg   >  2:630560/1‑140 (MQ=255)                                                                                                                             gTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAATCTTCTTCTCAGCCAAATCCAGCGCAAAga  >  1:580804/1‑139 (MQ=255)                                                                                                                             gTGGACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAATCTTCTTCTCAGCCAAATCCAGCGCAAAGt  >  2:40418/1‑140 (MQ=255)                                                                                                                                gACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAATCTTCTTCTCAGCCAAATCCAGCGCAAAg   <  2:576569/136‑1 (MQ=255)                                                                                                                                gACGGTCCCTTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAATCTTCTTCTCAGCCAAATCCAGCGCAAAg   >  1:576569/1‑136 (MQ=255)                                                                                                                                         |                                                                                                                                GCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAATCTTCTTCTCAGCCAAATCCAGCGCAAAGT  >  NC_000913/388530‑388793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑