Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	469,238	A→G	D123G (GAC→GGC)	mdlA →	ABC transporter family protein MdlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	469,238	0	A	G	100.0%	210.0 / NA	57	D123G (GAC→GGC)	mdlA	ABC transporter family protein MdlA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/34);  total (23/34)

TGGTGCGTCTTATCAACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCC  >  NC_000913/469116‑469370                                                                                                                           |                                                                                                                                      tGGTGCGTCTTATCAACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCgtgt                                                                                                                      >  1:526323/1‑140 (MQ=255)   gTGCGTCTTATCAACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGttt                                                                                                                    <  2:29665/140‑1 (MQ=255)    tGCGTCTTATCAACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTg                                                                                                                   >  2:670393/1‑140 (MQ=255)      cGTCTTATCAACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTgcc                                                                                                                 >  2:472368/1‑140 (MQ=255)       gTCTTATCAACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTgccg                                                                                                                >  1:804187/1‑140 (MQ=255)        tCTTATCAACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTgccgc                                                                                                               <  1:52730/140‑1 (MQ=255)             tCAACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGgag                                                                                                          <  1:44038/140‑1 (MQ=255)              cAACTGGCTATTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGgaga                                                                                                         <  2:422500/140‑1 (MQ=255)                   ggCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAgggg                                                                                                    >  1:133955/1‑140 (MQ=255)                    gCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAggggg                                                                                                   >  2:976786/1‑139 (MQ=255)                    gCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAggggg                                                                                                   >  2:471459/1‑139 (MQ=255)                        ttGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGGGCCACCCATGGCGTCGATCGCGTCGTGTTTGCCGCCGGGGAAGGGGGGcgg                                                                                               >  2:36939/1‑138 (MQ=255)                         tGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGa                                                                                              <  1:308138/140‑1 (MQ=255)                           aaCTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACg                                                                                            <  1:1031796/140‑1 (MQ=255)                              tGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCtg                                                                                         >  2:360653/1‑140 (MQ=255)                              tGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCtg                                                                                         <  2:413290/140‑1 (MQ=255)                                cGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCtggt                                                                                       <  2:176259/140‑1 (MQ=255)                                cGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCtggt                                                                                       <  1:180873/140‑1 (MQ=255)                                cGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCtggg                                                                                       >  1:891142/1‑139 (MQ=255)                                  tGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCtggtgg                                                                                     >  2:406817/1‑140 (MQ=255)                                        ttattaCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCAc                                                                               >  2:1008504/1‑140 (MQ=255)                                          attaCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGGGAAGGGGGGCTGACGCTGGTGGATTCACTg                                                                             >  2:946955/1‑140 (MQ=255)                                                  cAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATggg                                                                     <  1:263013/140‑1 (MQ=255)                                                   aGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGc                                                                    >  2:188569/1‑140 (MQ=255)                                                     cTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTg                                                                  >  1:581830/1‑140 (MQ=255)                                                     cTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGACGATCGCGTCGAGGTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGGGATGGGCTg                                                                  >  2:292793/1‑140 (MQ=255)                                                       aggCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTgcg                                                                <  1:331159/138‑1 (MQ=255)                                                       aaGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTgcg                                                                <  2:884637/140‑1 (MQ=255)                                                       aaGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTgcg                                                                <  2:804187/140‑1 (MQ=255)                                                       aaGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTgcg                                                                <  2:526323/140‑1 (MQ=255)                                                         gCCGGCAGCATCCTGAGTTTTATCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCt                                                              <  1:863536/140‑1 (MQ=255)                                                         gCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGCCCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCt                                                              <  1:1010062/140‑1 (MQ=255)                                                         gCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCt                                                              <  2:479349/140‑1 (MQ=255)                                                              cagcaTCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGGGATGGGCTGCGCTGTGtt                                                         >  1:245026/1‑140 (MQ=255)                                                               agcaTCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTg                                                        <  2:596282/140‑1 (MQ=255)                                                               agcaTCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTg                                                        >  1:388938/1‑140 (MQ=255)                                                                    ccTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTat                                                  <  2:133955/140‑1 (MQ=255)                                                                     cTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTatg                                                 <  1:608844/140‑1 (MQ=255)                                                                     cTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTatg                                                 <  1:472368/140‑1 (MQ=255)                                                                         gTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTATGATGt                                             <  1:406817/140‑1 (MQ=255)                                                                                  gCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGGTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTATGATGTCTACGCaaa                                    >  2:772827/1‑140 (MQ=255)                                                                                     tCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTATGATGTCTACGCAAATTa                                 <  2:1038649/140‑1 (MQ=255)                                                                                       aTCGCACCGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGGGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTATGATGTCTACGCAAATTAGc                               >  2:274065/1‑140 (MQ=255)                                                                                             ccGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTATGATGTCTACGCAAATTAGCTGGCAg                         <  1:188569/140‑1 (MQ=255)                                                                                             ccGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGGGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTATGATGTCTACGCAAATTAGCTGGCAg                         >  1:663901/1‑140 (MQ=255)                                                                                              cGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTATGATGTCTACGCAAATTAGCTGGCAGt                        <  2:533636/140‑1 (MQ=255)                                                                                              cGGTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTATGATGTCTACGCAAATTAGCTGGCAGt                        <  2:84612/140‑1 (MQ=255)                                                                                               ggTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTATGATGTCTACGCAAATTAGCTGGCAGtt                       <  2:891142/140‑1 (MQ=255)                                                                                                gTGACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTATGATGTCTACGCAAATTAGCTGGTAGTTg                      >  2:347505/1‑140 (MQ=255)                                                                                                  gACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTATGATGTCTACGCAAATTAGCTGGCAGTTGAc                    <  1:25853/140‑1 (MQ=255)                                                                                                  gACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTATGATGTCTACGCAAATTAGCTGGCAGTTGAc                    <  2:245026/140‑1 (MQ=255)                                                                                                  gACCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTATGATGTCTACGCAAATTAGCTGGCAGTTGAc                    <  2:581830/140‑1 (MQ=255)                                                                                                   aCCTCATGGCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTATGATGTCTACGCAAATTAGCTGGCAGTTGAcc                   <  2:34640/140‑1 (MQ=255)                                                                                                          ggCTCGTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTAtttt            >  2:657086/1‑140 (MQ=255)                                                                                                               gTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTg       <  2:316992/140‑1 (MQ=255)                                                                                                               gTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTg       >  1:922253/1‑140 (MQ=255)                                                                                                               gTGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCGGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTg       <  1:829767/140‑1 (MQ=255)                                                                                                                tGCGACCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTg       >  1:878146/1‑140 (MQ=255)                                                                                                                tGCGACCAATAGTGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGGGCTGACGCCCGTGGATTCACTGGTGGTGGGCCGCGCTGTGTTGA‑TTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGt      >  2:232058/1‑140 (MQ=255)                                                                                                                    aCCAATGGCGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGcc  <  1:74324/140‑1 (MQ=255)                                                                                                                           |                                                                                                                                      TGGTGCGTCTTATCAACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGA‑TTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCC  >  NC_000913/469116‑469370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑