Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	518,198	A→G	F28L (TTC→CTC)	cnoX ←	chaperedoxin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	518,198	0	A	G	100.0%	186.6 / NA	50	F28L (TTC→CTC)	cnoX	chaperedoxin
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/21);  total (29/21)

GGCAATCATCTGCTCCGCGTCGCAGTCCAGCTTCGCCAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAACAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGC  >  NC_000913/518070‑518333                                                                                                                                 |                                                                                                                                        ggCAATCATCTGCTCCGCGTCGCAGTCCAGCTTCGCCAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAg                                                                                                                              >  2:294577/1‑140 (MQ=255) ggCAATCATCTGCTCCGCGTCGCAGTCCAGCTTCGCCAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAg                                                                                                                              >  2:309781/1‑140 (MQ=255)         tcTGCTCCGCGTCGCAGTCCAGCTTCGCCAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATc                                                                                                                      <  1:982173/140‑1 (MQ=255)           tGCTCCGCGTCGCAGTCCAGCTTCGCCAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGa                                                                                                                    <  1:204194/140‑1 (MQ=255)           tGCTCCGCGTCGCAGTCCAGCTTCGCCAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGa                                                                                                                    <  1:210199/140‑1 (MQ=255)           tGCTCCGCGTCGCAGTCCAGCTTCGCCAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGa                                                                                                                    <  2:252954/140‑1 (MQ=255)             cTCCGCGTCGCAGTCCAGCTTCGCCAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACt                                                                                                                  >  1:595201/1‑140 (MQ=255)             cTCCGCGTCGCAGTCCAGCTTCGCCAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACt                                                                                                                  >  1:78822/1‑140 (MQ=255)                cgcgTCGCAGTCCAGCTTCGCCAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGtt                                                                                                               >  2:372684/1‑140 (MQ=255)                cgcgTCGCAGTCCAGCTTCGCCAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGtt                                                                                                               >  2:473964/1‑140 (MQ=255)                  cgTCGCAGTCCAGCTTCGCCAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTTGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGa                                                                                                                    <  1:718568/133‑1 (MQ=255)                  cgTCGCAGTCCAGCTTCGCCAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTTGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGa                                                                                                                    >  2:718568/1‑133 (MQ=255)                          tCCAGCTTCGCCAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTg                                                                                                     >  1:666793/1‑140 (MQ=255)                              gCTTCGCCAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGc                                                                                                 <  1:157692/140‑1 (MQ=255)                               cTTCGCTAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGCGGTCATCTACTGTTCAAGAACCTGTTGCa                                                                                                >  1:128547/1‑140 (MQ=255)                               cTTCGCCAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGAGTGTTCAAGAACCTGTTGCa                                                                                                >  1:774544/1‑140 (MQ=255)                                 tCGCCAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAgg                                                                                              <  1:441784/140‑1 (MQ=255)                                  cGCCAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGt                                                                                             <  2:756542/140‑1 (MQ=255)                                    ccAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTa                                                                                           >  1:793887/1‑140 (MQ=255)                                    ccAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTa                                                                                           >  1:799963/1‑140 (MQ=255)                                              ttGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCTGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTaa                                                                                 <  2:541739/140‑1 (MQ=255)                                                   cGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTg                                                                            <  1:874488/140‑1 (MQ=255)                                                   cGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTg                                                                            <  2:193449/140‑1 (MQ=255)                                                               ccgcGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCt                                                                >  1:758634/1‑140 (MQ=255)                                                                cgcgAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTa                                                               <  1:372684/140‑1 (MQ=255)                                                                cgcgAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAACACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTa                                                               >  1:869948/1‑140 (MQ=255)                                                                 gcgAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTAc                                                              <  2:799963/140‑1 (MQ=255)                                                                  cgAGGCTTTCCAGAATTGGGGTTATCTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACg                                                             >  2:769061/1‑140 (MQ=255)                                                                  cgAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACg                                                             >  2:581557/1‑140 (MQ=255)                                                                  cgAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACg                                                             >  1:599/1‑140 (MQ=255)                                                                  cgAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACg                                                             >  2:798772/1‑140 (MQ=255)                                                                  cgAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACg                                                             >  2:208637/1‑140 (MQ=255)                                                                          tCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGa                                                     <  1:842184/140‑1 (MQ=255)                                                                           ccAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAg                                                    <  1:710234/140‑1 (MQ=255)                                                                                   ggggTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGctctc                                            <  1:513551/140‑1 (MQ=255)                                                                                    gggTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGctctct                                           >  2:179698/1‑140 (MQ=255)                                                                                    gggTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGctccct                                           >  2:802244/1‑140 (MQ=255)                                                                                                     tGCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCtt                          >  1:299372/1‑140 (MQ=255)                                                                                                      gCTGGCTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCttt                         >  1:711316/1‑140 (MQ=255)                                                                                                           cTACGTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACAt                    <  1:1022065/140‑1 (MQ=255)                                                                                                               gTTCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATgggg                >  1:664369/1‑140 (MQ=255)                                                                                                                 tCAGACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGc              >  2:964703/1‑140 (MQ=255)                                                                                                                    gACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTa           >  1:966088/1‑140 (MQ=255)                                                                                                                    gACCAAAAATAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTa           >  1:14726/1‑140 (MQ=255)                                                                                                                         aaaaTAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAgcgc      >  2:975550/1‑140 (MQ=255)                                                                                                                         aaaaTAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAgcgc      >  1:44741/1‑140 (MQ=255)                                                                                                                          aaaTAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAgcgcg     <  1:473964/140‑1 (MQ=255)                                                                                                                          aaaTAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAgcgcg     <  2:468610/140‑1 (MQ=255)                                                                                                                          aaaTAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAgcgcg     <  1:769061/140‑1 (MQ=255)                                                                                                                          aaaTAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAgcgcg     <  1:959489/140‑1 (MQ=255)                                                                                                                             tAGAGCAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAgcgcgcgc  <  2:666793/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                        GGCAATCATCTGCTCCGCGTCGCAGTCCAGCTTCGCCAGAATAAATTGCCCGTTGTACTGCGCCGCGAGGCTTTCCAGAATTGGGGTTAACTGCAAACAGTGCTGGCTACGTTCAGACCAAAAATAGAACAGCACCGGAGTGGTCATCGACTGTTCAAGAACCTGTTGCAGGTTAGATTCGTTAATGTTGACAATATTTTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGC  >  NC_000913/518070‑518333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑