Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	707,786	G→A	intergenic (+29/‑548)	glnS → / → chiP	glutamine‑‑tRNA ligase/chitobiose outer membrane channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	707,786	0	G	A	100.0%	195.6 / NA	57	intergenic (+29/‑548)	glnS/chiP	glutamine‑‑tRNA ligase/chitobiose outer membrane channel
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/37);  total (20/37)

TGAAGGTTACTTCTGCCTCGATAGCCGCCATTCTACGGCGGAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCGTTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTC  >  NC_000913/707655‑707920                                                                                                                                    |                                                                                                                                       tGAAGGTTACTTCTGCCTCGATAGCCGCCATTCTACGGCGGAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTc                                                                                                                                >  1:842405/1‑140 (MQ=255)   aaGGTTACTTCTGCCTCGATAGCCGCCATTCTACGGCGGAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGc                                                                                                                              >  1:303798/1‑140 (MQ=255)    aGGTTACTTCTGCCTCGATAGCCGCCATTCTACGGCGGAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCa                                                                                                                             <  2:227958/140‑1 (MQ=255)           ttCTGCCTCGATAGCCGCCATTCTACGGCGGAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCa                                                                                                                      <  2:239610/140‑1 (MQ=255)              tGCCTCGATAGCCGCCATTCTACGGCGGAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAgg                                                                                                                   <  1:581959/140‑1 (MQ=255)              tGCCTCGATAGCCGCCATTCTACGGCGGAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAgg                                                                                                                   <  1:379155/140‑1 (MQ=255)                ccTCGATAGCCGCCATTCTACGGCGGAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCa                                                                                                                      <  1:580362/135‑1 (MQ=255)                ccTCGATAGCCGCCATTCTACGGCGGAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCa                                                                                                                      >  2:580362/1‑135 (MQ=255)                ccTCGATAGCCGCCATTCTACGGCGGAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGaa                                                                                                                 >  2:982728/1‑140 (MQ=255)                ccTCGATAGCCGCCATTCTACGGCGGAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGaa                                                                                                                 >  2:399087/1‑140 (MQ=255)                ccTCGATAGCCGCCATTCTACGGCGGAAAAACCGGAATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGaa                                                                                                                 >  1:243884/1‑140 (MQ=255)                     aTAGCCGCCATTCTACGGCGGAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGAc                                                                                                            >  1:209215/1‑140 (MQ=255)                         ccgccATTCTACGGCGGAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAAc                                                                                                        >  1:965619/1‑140 (MQ=255)                         ccgccATTCTACGGCGGAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGGTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAAc                                                                                                        >  1:21943/1‑140 (MQ=255)                          cgccATTCTACGGCGGAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACa                                                                                                       >  2:543730/1‑140 (MQ=255)                               ttCTACGGCGGAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTc                                                                                                  <  1:676365/140‑1 (MQ=255)                                  tACGGCGGAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCaaa                                                                                               <  2:712145/140‑1 (MQ=255)                                   aCGGCGGAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAAc                                                                                              <  2:976927/140‑1 (MQ=255)                                   aCGGCGGAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAAc                                                                                              <  1:982728/140‑1 (MQ=255)                                    cggcggAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACg                                                                                             <  1:552380/140‑1 (MQ=255)                                    cggcggAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACg                                                                                             <  2:248853/140‑1 (MQ=255)                                    cggcggAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACg                                                                                             <  1:701089/140‑1 (MQ=255)                                    cggcggAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACg                                                                                             >  1:760673/1‑140 (MQ=255)                                         gAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTaaa                                                                                        >  1:964417/1‑140 (MQ=255)                                                     tttAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATgcg                                                                            <  2:546279/140‑1 (MQ=255)                                                     tttAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATgcg                                                                            <  2:513352/140‑1 (MQ=255)                                                     tttAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATgcg                                                                            <  2:965619/140‑1 (MQ=255)                                                     tttAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATgcg                                                                            <  2:772961/140‑1 (MQ=255)                                                        aaCCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTa                                                                         >  2:194996/1‑140 (MQ=255)                                                          ccGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACg                                                                       <  1:962159/140‑1 (MQ=255)                                                          ccGCACCATTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACg                                                                       <  2:573562/140‑1 (MQ=255)                                                             cACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCtt                                                                    >  2:630685/1‑140 (MQ=255)                                                               ccGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTAt                                                                  <  2:32652/140‑1 (MQ=255)                                                               ccGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTAt                                                                  <  2:406378/140‑1 (MQ=255)                                                               ccGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTAt                                                                  <  2:466252/140‑1 (MQ=255)                                                               ccGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTAt                                                                  <  2:537360/140‑1 (MQ=255)                                                               ccGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTAt                                                                  <  1:298250/140‑1 (MQ=255)                                                                 gTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCa                                                                <  2:21943/140‑1 (MQ=255)                                                                  ttGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAg                                                               <  1:325238/140‑1 (MQ=255)                                                                          cGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAt                                                       >  1:740883/1‑140 (MQ=255)                                                                          cGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAt                                                       >  1:385652/1‑140 (MQ=255)                                                                           gTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATg                                                      <  2:741105/140‑1 (MQ=255)                                                                             gATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGAt                                                    <  2:242612/140‑1 (MQ=255)                                                                             gATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGAt                                                    <  1:586317/140‑1 (MQ=255)                                                                               tACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGAtct                                                  >  2:1017264/1‑140 (MQ=255)                                                                                    gggCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATCTCTGCa                                             <  2:209215/140‑1 (MQ=255)                                                                                    gggCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATCTCTGCa                                             <  2:243884/140‑1 (MQ=255)                                                                                     ggCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATCTCTGCaa                                            <  1:690335/140‑1 (MQ=255)                                                                                                  gAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATCTCTGCAATATATTGAGTTTg                               >  2:477093/1‑140 (MQ=255)                                                                                                  gAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATCTCTGCAATATATTGAGTTTg                               <  1:169645/140‑1 (MQ=255)                                                                                                    gTAATTTTAAGTTTCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATCTCTGCAATATATTGAGTTTGCg                             >  1:8563/1‑140 (MQ=255)                                                                                                               tttCGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAg                  <  1:175141/140‑1 (MQ=255)                                                                                                                  cGCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGcc               >  1:837443/1‑140 (MQ=255)                                                                                                                   gCTATGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTCATCAGGCCTACATGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCg              <  2:760673/140‑1 (MQ=255)                                                                                                                      aTGCCGGATGGGGCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGAt           <  2:842405/140‑1 (MQ=255)                                                                                                                               ggggCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTc  <  2:558415/140‑1 (MQ=255)                                                                                                                               ggggCATTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTc  <  2:435166/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       TGAAGGTTACTTCTGCCTCGATAGCCGCCATTCTACGGCGGAAAAACCGGTATTTAACCGCACCGTTGGGCTGCGTGATACCTGGGCGAAAGTAGGCGAGTAATTTTAAGTTTCGCTATGCCGGATGGGGCGTTTACGTCGCATCCGGCAAGGAACAGACAAACAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTC  >  NC_000913/707655‑707920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑