Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	796,417	T→C	Y186H (TAT→CAT)	modB →	molybdate ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	796,417	0	T	C	100.0%	119.9 / NA	33	Y186H (TAT→CAT)	modB	molybdate ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/14);  total (19/14)

CGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGG  >  NC_000913/796283‑796543                                                                                                                                       |                                                                                                                               cGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATAcc                                                                                                                           <  1:192808/140‑1 (MQ=255) cGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATAcc                                                                                                                           >  1:242902/1‑140 (MQ=255)        gCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATcc                                                                                                                    <  1:289619/140‑1 (MQ=255)            aCCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGAc                                                                                                                >  2:971767/1‑140 (MQ=255)            aCCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGAc                                                                                                                >  2:251350/1‑140 (MQ=255)                              gttgGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTgg                                                                                              <  2:187174/140‑1 (MQ=255)                               ttgGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTggg                                                                                             >  1:114828/1‑139 (MQ=255)                                 ggtacggtacTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAgc                                                                                           >  2:101069/1‑140 (MQ=255)                                     cggtacTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAgcggcg                                                                                       <  2:252211/140‑1 (MQ=255)                                        tacTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCgaga                                                                                    >  1:857674/1‑140 (MQ=255)                                            ggCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTgg                                                                                              >  2:133123/1‑126 (MQ=255)                                            ggCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTgg                                                                                              <  1:133123/126‑1 (MQ=255)                                             gCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACtgtg                                                                               >  1:769707/1‑140 (MQ=255)                                              cTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGc                                                                              <  2:813261/140‑1 (MQ=255)                                                  tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGTCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCatta                                                                          >  2:459738/1‑140 (MQ=255)                                                  tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCcttt                                                                          >  1:133814/1‑139 (MQ=255)                                                  tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCatta                                                                          >  1:617064/1‑140 (MQ=255)                                                  tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCatta                                                                          >  2:9595/1‑140 (MQ=255)                                                  tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCatta                                                                          >  1:359010/1‑140 (MQ=255)                                                                  gAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGc                                                          <  1:908780/140‑1 (MQ=255)                                                                  gAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGc                                                          <  1:168982/140‑1 (MQ=255)                                                                               ccATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTg                                             >  1:49229/1‑140 (MQ=255)                                                                               ccATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTg                                             >  1:792981/1‑140 (MQ=255)                                                                                    aCCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGAt                                        <  1:101069/140‑1 (MQ=255)                                                                                             tCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAAtg                               >  1:467811/1‑140 (MQ=255)                                                                                                aaCATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAAtggct                            >  1:971875/1‑140 (MQ=255)                                                                                                        tGGTGAAACGCGAACCATTCCTTCTGTCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGaa                    <  1:459738/140‑1 (MQ=255)                                                                                                            gAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAg                <  2:617064/140‑1 (MQ=255)                                                                                                             aaaCGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGc               >  1:663429/1‑140 (MQ=255)                                                                                                               aCGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCg             >  1:532325/1‑140 (MQ=255)                                                                                                                         ttCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAAcgggcg   <  1:9595/140‑1 (MQ=255)                                                                                                                         ttCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAAcgggcg   <  2:792981/140‑1 (MQ=255)                                                                                                                          tCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAAcgggcgg  <  1:251350/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                               CGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGG  >  NC_000913/796283‑796543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑