Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	920,764	T→C	Y140H (TAT→CAT)	macB →	ABC‑type tripartite efflux pump ATP binding/membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	920,764	0	T	C	100.0%	162.9 / NA	44	Y140H (TAT→CAT)	macB	ABC‑type tripartite efflux pump ATP binding/membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/24);  total (20/24)

ACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGA  >  NC_000913/920630‑920896                                                                                                                                       |                                                                                                                                     aCCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGcattat                                                                                                                                 >  1:523494/1‑140 (MQ=255) aCCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGcattat                                                                                                                                 >  2:706327/1‑140 (MQ=255)       tGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGca                                                                                                                           <  2:138799/140‑1 (MQ=255)       tGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGca                                                                                                                           <  1:19080/140‑1 (MQ=255)       tGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGca                                                                                                                           <  1:35057/140‑1 (MQ=255)       tGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGca                                                                                                                           <  2:62980/140‑1 (MQ=255)       tGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGca                                                                                                                           >  1:642705/1‑140 (MQ=255)            tCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCt                                                                                                                      >  2:164337/1‑140 (MQ=255)            tCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCt                                                                                                                      >  2:487773/1‑140 (MQ=255)                  ttAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCggg                                                                                                                <  1:181548/140‑1 (MQ=255)                        gccgAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCa                                                                                                          >  1:241789/1‑140 (MQ=255)                            aGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCaa                                                                                                      >  2:721661/1‑140 (MQ=255)                               aGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAg                                                                                                   <  2:1038642/140‑1 (MQ=255)                               aGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAg                                                                                                   <  1:787114/140‑1 (MQ=255)                               aGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAg                                                                                                   <  1:606362/140‑1 (MQ=255)                               aGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAg                                                                                                   <  1:109005/140‑1 (MQ=255)                                      tGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCa                                                                                            >  1:408693/1‑140 (MQ=255)                                        aaGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGc                                                                                          >  2:223095/1‑140 (MQ=255)                                          gTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGTGGCTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGGTGGAAGACCGTGCAGAGCATTATCCGGCACAGCTTTCGGGTGGGCATCTACAGCGCGTCAGCAt                                                                                        <  2:940006/140‑1 (MQ=255)                                           tACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATc                                                                                       >  2:68374/1‑140 (MQ=255)                                           tACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATc                                                                                       >  1:532172/1‑140 (MQ=255)                                           tACCCGCCGTCTATGCTGGCCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATc                                                                                       >  2:1035177/1‑140 (MQ=255)                                              ccgccgTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg                                                                                    <  1:721661/140‑1 (MQ=255)                                              ccgccgTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGGCAGCAACAGCGCGTCAGCATcgcg                                                                                    >  2:969998/1‑140 (MQ=255)                                               cgccgTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcgc                                                                                   <  2:985296/140‑1 (MQ=255)                                                gccgTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcgcg                                                                                  >  2:836428/1‑140 (MQ=255)                                                  cgTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCggg                                                                                >  1:453114/1‑140 (MQ=255)                                                  cgTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCggg                                                                                <  2:532172/140‑1 (MQ=255)                                                    tCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCa                                                                              <  1:680483/140‑1 (MQ=255)                                                            ggTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGaa                                                                      <  1:903789/140‑1 (MQ=255)                                                              tCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACg                                                                    >  2:484384/1‑140 (MQ=255)                                                              tCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCAGTAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACg                                                                    <  1:263991/140‑1 (MQ=255)                                                                  gAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACggtgg                                                                <  2:731844/140‑1 (MQ=255)                                                                  gAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACggtgg                                                                <  2:523494/140‑1 (MQ=255)                                                                   aGCGGAAACAGCGACTGCTACGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACggtggt                                                               <  1:690181/140‑1 (MQ=255)                                                                              cGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCt                                                    <  2:642705/140‑1 (MQ=255)                                                                               gACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGGGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCtt                                                   >  1:85271/1‑140 (MQ=255)                                                                                   gCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCg                                               <  1:113144/140‑1 (MQ=255)                                                                                               ggAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCg                                   <  1:917843/140‑1 (MQ=255)                                                                                               ggAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCg                                   <  2:202087/140‑1 (MQ=255)                                                                                                    tgctgcAACGGCTGGGGCTGGAAGACTGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCa                              <  2:453114/140‑1 (MQ=255)                                                                                                                    gctggAAGACCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTg              >  2:931959/1‑140 (MQ=255)                                                                                                                            aCCGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAagag      <  1:68374/140‑1 (MQ=255)                                                                                                                              cGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAggg    >  2:542722/1‑139 (MQ=255)                                                                                                                              cGTACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt    >  1:791540/1‑140 (MQ=255)                                                                                                                                tACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGa  >  1:861066/1‑140 (MQ=255)                                                                                                                                tACAGAGCATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGATCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGa  >  2:810539/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                     ACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGA  >  NC_000913/920630‑920896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑