Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	91,264	A→G	D78G (GAC→GGC)	ftsL →	cell division protein FtsL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	91,264	0	A	G	100.0%	191.9 / NA	52	D78G (GAC→GGC)	ftsL	cell division protein FtsL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/33);  total (19/33)

GATTTGGGAAGCTGCCACTCTGCCTGTTCATTTGCATTATTTTGACGGCGGTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAAAATA  >  NC_000913/91132‑91396                                                                                                                                     |                                                                                                                                     gATTTGGGAAGCTGCCACTCTGCCTGTTCATTTGCATTATTTTGACGGCGGTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGaa                                                                                                                               <  1:405752/140‑1 (MQ=255)    ttGGGAAGCTGCCACTCTGCCTGTTCATTTGCATTATTTTGACGGCGGTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATgg                                                                                                                            >  1:617005/1‑140 (MQ=255)     tGGGAAGCTGCCACTCTGCCTGTTCATTTGCATTATTTTGACGGCGGTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGgc                                                                                                                           >  1:386828/1‑140 (MQ=255)      gggAAGCTGCCACTCTGCCTGTTCATTTGCATTATTTTGACGGCGGTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGgcg                                                                                                                          <  2:707516/140‑1 (MQ=255)      gggAAGCTGCCACTCTGCCTGTTCATTTGCATTATTTTGACGGCGGTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGgcg                                                                                                                          <  1:698972/140‑1 (MQ=255)            cTGCCACTCTGCCTGTTCATTTGCATTATTTTGACGGCGGTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCt                                                                                                                    >  2:705718/1‑140 (MQ=255)            cTGCCACTCTGCCTGTTCATTTGCATTATTTTGACGGCGGTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCt                                                                                                                    <  1:126418/140‑1 (MQ=255)            cTGCCACTCTGCCTGTTCATTTGCATTATTTTGACGGCGGTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCt                                                                                                                    <  2:720834/140‑1 (MQ=255)                   tctGCCTGTTCATTTGCATTATTTTGACGGCGGTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCtt                                                                                                             <  1:892061/140‑1 (MQ=255)                   tctGCCTGTTCATTTGCATTATTTTGACGGCGGTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCtt                                                                                                             <  1:419881/140‑1 (MQ=255)                       ccTGTTCATTTGCATTATTTTGACGGCGGTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAag                                                                                                         >  2:504958/1‑140 (MQ=255)                            tCATTTGCATTATTTTGACGGCGGTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAAt                                                                                                    >  1:458460/1‑140 (MQ=255)                                 tGCATTATTTTGACGGCGGTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATgcgcc                                                                                               >  1:175732/1‑139 (MQ=255)                                   cATTATTTTGACGGCGGTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCg                                                                                             <  2:172409/140‑1 (MQ=255)                                             aCGGCGGTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAg                                                                                   <  1:784632/140‑1 (MQ=255)                                             aCGGCGGTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAg                                                                                   <  2:585582/140‑1 (MQ=255)                                              cggcggTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGc                                                                                  <  1:552196/140‑1 (MQ=255)                                              cggcggTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGc                                                                                  <  1:7062/140‑1 (MQ=255)                                                 cggTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCgg                                                                               <  2:679125/140‑1 (MQ=255)                                                  ggTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCggg                                                                              <  2:422610/140‑1 (MQ=255)                                                           ggTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGa                                                                     >  1:285926/1‑140 (MQ=255)                                                                  aCGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCAc                                                              >  2:424463/1‑140 (MQ=255)                                                                    ggCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACgg                                                            >  1:1021212/1‑140 (MQ=255)                                                                     gcgcACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGa                                                           <  2:382779/140‑1 (MQ=255)                                                                     gcgcACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCACCACGGa                                                           >  2:1029565/1‑140 (MQ=255)                                                                        cACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGaaaa                                                        <  2:452178/140‑1 (MQ=255)                                                                         aCCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAg                                                       >  2:247827/1‑140 (MQ=255)                                                                            aTACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTg                                                    <  1:680369/140‑1 (MQ=255)                                                                                  gTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATg                                              <  1:94890/140‑1 (MQ=255)                                                                                   tttACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATgc                                             >  1:905602/1‑140 (MQ=255)                                                                                   tttACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATgc                                             >  1:867009/1‑140 (MQ=255)                                                                                   tttACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATgc                                             >  2:651207/1‑140 (MQ=255)                                                                                    ttACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATgca                                            <  1:183761/140‑1 (MQ=255)                                                                                    ttACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATgca                                            <  1:255026/140‑1 (MQ=255)                                                                                    ttACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATgca                                            <  1:356472/140‑1 (MQ=255)                                                                                    ttACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATgca                                            <  1:568598/140‑1 (MQ=255)                                                                                       cTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATgcagca                                         >  2:330812/1‑140 (MQ=255)                                                                                         gACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATg                                       <  2:501644/140‑1 (MQ=255)                                                                                           ccGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGtt                                     >  1:932927/1‑140 (MQ=255)                                                                                            cGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTg                                    >  2:117740/1‑140 (MQ=255)                                                                                              cTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGAt                                  >  1:642663/1‑140 (MQ=255)                                                                                               tCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATc                                 <  2:458460/140‑1 (MQ=255)                                                                                               tCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATc                                 >  1:392898/1‑140 (MQ=255)                                                                                                     cgAACAACTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTcac                           <  2:415414/140‑1 (MQ=255)                                                                                                       aacaacTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACaa                         <  1:705718/140‑1 (MQ=255)                                                                                                       aacaacTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACaa                         <  1:248294/140‑1 (MQ=255)                                                                                                       aacaacTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACaa                         <  1:121880/140‑1 (MQ=255)                                                                                                           acTGGTGCTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCACCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGaaa                     <  1:1029565/140‑1 (MQ=255)                                                                                                                  cTGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGt              <  2:867009/140‑1 (MQ=255)                                                                                                                   tGGAGCGAGATGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTa             <  1:504958/140‑1 (MQ=255)                                                                                                                             tGCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAAAAt   <  2:642663/140‑1 (MQ=255)                                                                                                                              gCTTTAGGCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAAAATa  <  2:76780/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                     GATTTGGGAAGCTGCCACTCTGCCTGTTCATTTGCATTATTTTGACGGCGGTGACTGTGGTAACCACGGCGCACCATACCCGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAAAATA  >  NC_000913/91132‑91396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑