Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	921,992	C→T	T549I (ACC→ATC)	macB →	ABC‑type tripartite efflux pump ATP binding/membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	921,992	0	C	T	100.0%	189.3 / NA	55	T549I (ACC→ATC)	macB	ABC‑type tripartite efflux pump ATP binding/membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/29);  total (26/29)

GAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGACCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACT  >  NC_000913/921861‑922121                                                                                                                                    |                                                                                                                                  gAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGa                                                                                                                           >  2:166069/1‑140 (MQ=255)  aaCATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGAc                                                                                                                          >  2:770137/1‑140 (MQ=255)       ggACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCggg                                                                                                                     >  1:81035/1‑140 (MQ=255)       ggACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCggg                                                                                                                     >  1:368258/1‑140 (MQ=255)       ggACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCggg                                                                                                                     >  2:56895/1‑140 (MQ=255)            gCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAAtt                                                                                                                >  2:916032/1‑140 (MQ=255)                     aaaCTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGc                                                                                                       <  2:418991/140‑1 (MQ=255)                     aaaCTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGc                                                                                                       <  1:770137/140‑1 (MQ=255)                     aaaCTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGc                                                                                                       <  1:460285/140‑1 (MQ=255)                     aaaCTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGc                                                                                                       <  1:72830/140‑1 (MQ=255)                     aaaCTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGc                                                                                                       <  2:718826/140‑1 (MQ=255)                     aaaCTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGc                                                                                                       <  1:530522/140‑1 (MQ=255)                     aaaCTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGc                                                                                                       <  1:616190/140‑1 (MQ=255)                      aaCTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCa                                                                                                      <  2:584370/140‑1 (MQ=255)                       aCTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCAt                                                                                                     <  2:342156/140‑1 (MQ=255)                         tGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATgg                                                                                                   <  2:58898/140‑1 (MQ=255)                          gTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGc                                                                                                  >  2:52606/1‑140 (MQ=255)                             gAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCA‑‑GATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAg                                                                                             <  1:149637/140‑1 (MQ=255)                                   aCCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTgc                                                                                         >  2:565814/1‑140 (MQ=255)                                     cacaCGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGGGGTGGGCGGTATTGGTGTAATGGATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTgcgc                                                                                       >  2:216978/1‑140 (MQ=255)                                           tACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGACATTCGCATGGCTGTAGGTGCGCGAGCaa                                                                                 <  2:95300/140‑1 (MQ=255)                                               ttACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGa                                                                             <  2:9649/140‑1 (MQ=255)                                               ttACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGa                                                                             <  2:128887/140‑1 (MQ=255)                                               ttACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGa                                                                             <  2:393235/140‑1 (MQ=255)                                                 aCAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATg                                                                           <  2:368258/140‑1 (MQ=255)                                                  cAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGt                                                                          >  1:543466/1‑140 (MQ=255)                                                      tGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTg                                                                      >  1:321841/1‑140 (MQ=255)                                                       gTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGc                                                                     >  2:8558/1‑140 (MQ=255)                                                         ttCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCaa                                                                   <  1:166069/140‑1 (MQ=255)                                                         ttCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCaa                                                                   <  1:519001/140‑1 (MQ=255)                                                          tCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAAc                                                                  <  2:102269/140‑1 (MQ=255)                                                           cTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACa                                                                 <  1:980029/140‑1 (MQ=255)                                                            tGACGATGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAg                                                                <  1:464731/140‑1 (MQ=255)                                                                  tggtggCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTg                                                          >  2:789646/1‑140 (MQ=255)                                                                    gtggCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGAt                                                        >  1:489360/1‑140 (MQ=255)                                                                         ggTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAg                                                   <  1:565814/140‑1 (MQ=255)                                                                         ggTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAg                                                   <  1:56895/140‑1 (MQ=255)                                                                              tttCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTa                                              >  1:157181/1‑140 (MQ=255)                                                                                       tggtggGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGc                                     >  2:811497/1‑140 (MQ=255)                                                                                         gtggGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCt                                   >  1:669884/1‑140 (MQ=255)                                                                                                gTATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGt                            <  1:52606/140‑1 (MQ=255)                                                                                                 tATTGGTGTAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTg                           >  2:590655/1‑140 (MQ=255)                                                                                                      gtgtAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGcgcg                      >  2:76266/1‑140 (MQ=255)                                                                                                        gtAATGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGtt                    <  1:789646/140‑1 (MQ=255)                                                                                                           aTGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGTTggg                 >  2:1035553/1‑140 (MQ=255)                                                                                                           aTGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGTTggg                 >  1:875175/1‑140 (MQ=255)                                                                                                           aTGAATATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGTTggg                 >  2:525693/1‑140 (MQ=255)                                                                                                              aaTATTATGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGTTGGGAAt              <  2:489360/140‑1 (MQ=255)                                                                                                                  ttatGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAaca          <  2:321841/140‑1 (MQ=255)                                                                                                                  ttatGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAaca          <  1:267996/140‑1 (MQ=255)                                                                                                                   tatGCTGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAacac         >  2:485622/1‑140 (MQ=255)                                                                                                                        tGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAAcactgtca    <  1:8558/140‑1 (MQ=255)                                                                                                                        tGGTGTCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAAcactgtca    >  1:910788/1‑140 (MQ=255)                                                                                                                          gtgtCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAAcactgtcact  >  1:922503/1‑140 (MQ=255)                                                                                                                          gtgtCAGTGATCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAAcactgtcact  >  1:269300/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                  GAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGACCGAGCGGACGCGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACT  >  NC_000913/921861‑922121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑