Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	927,208	G→A	S75S (AGC→AGT)	aat ←	leucyl/phenylalanyl‑tRNA‑‑protein transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	927,208	0	G	A	100.0%	167.6 / NA	50	S75S (AGC→AGT)	aat	leucyl/phenylalanyl‑tRNA‑‑protein transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/30);  total (20/30)

GGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCT  >  NC_000913/927072‑927333                                                                                                                                         |                                                                                                                              ggTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTg                                                                                                                            <  2:353291/140‑1 (MQ=255)    aGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGata                                                                                                                         <  1:109657/140‑1 (MQ=255)         tCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAg                                                                                                                    <  2:913498/140‑1 (MQ=255)              cacgccacgcGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGAtt                                                                                                               <  2:738641/140‑1 (MQ=255)              cacgccacgcGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGAtt                                                                                                               <  1:200054/140‑1 (MQ=255)              cacgccacgcGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGAtt                                                                                                               <  1:767098/140‑1 (MQ=255)              cacgccacgcGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGAtt                                                                                                               <  1:745279/140‑1 (MQ=255)                       cgTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATa                                                                                                      >  1:114802/1‑140 (MQ=255)                          gATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCa                                                                                                   <  1:792351/140‑1 (MQ=255)                           aTCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCAc                                                                                                  >  2:983473/1‑140 (MQ=255)                           aTCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCAc                                                                                                  >  2:852447/1‑140 (MQ=255)                           aTCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCAc                                                                                                  <  1:183901/140‑1 (MQ=255)                                    ccTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCgggg                                                                                         >  2:653369/1‑140 (MQ=255)                                      ttcttcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGAt                                                                                       >  1:324899/1‑140 (MQ=255)                                      ttcttcGCGATCGCTGGCACAGCATTCAATGACATGTCCAAAAGCGTAATCCATCGTGACACGATAGGGAGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACTGCGCGGGGAt                                                                                       <  2:59203/140‑1 (MQ=255)                                       tcttcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATc                                                                                      <  2:367457/140‑1 (MQ=255)                                          tcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCggg                                                                                   <  2:496052/140‑1 (MQ=255)                                           cgcgATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGc                                                                                  >  1:267300/1‑140 (MQ=255)                                           cgcgATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGAGc                                                                                  >  1:59203/1‑140 (MQ=255)                                                  gCTGGCACAGCCTTCAATGTCCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGTGATCGGGCGaccacc                                                                           <  2:820026/140‑1 (MQ=255)                                                        acaGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGACCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGa                                                                     >  2:517691/1‑140 (MQ=255)                                                          aGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATg                                                                   <  2:167137/140‑1 (MQ=255)                                                            ccTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGTTCGGGCGACCACCAGAGGATggg                                                                 >  1:420889/1‑140 (MQ=255)                                                            ccTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATggg                                                                 <  2:429841/140‑1 (MQ=255)                                                            ccTTCAAAGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATggg                                                                 >  2:209285/1‑140 (MQ=255)                                                             cTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATgggg                                                                >  2:11556/1‑140 (MQ=255)                                                                    gacctgaccAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTg                                                         >  1:961293/1‑140 (MQ=255)                                                                      cctgaccAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGga                                                       >  1:319895/1‑140 (MQ=255)                                                                               aaGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCAc                                              <  1:188593/140‑1 (MQ=255)                                                                                                  acGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGt                           <  2:684014/140‑1 (MQ=255)                                                                                                      tAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGc                       >  1:456958/1‑140 (MQ=255)                                                                                                             aGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAAc                >  1:564889/1‑140 (MQ=255)                                                                                                              gCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACa               >  2:739790/1‑140 (MQ=255)                                                                                                               cGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAg              >  2:1022578/1‑140 (MQ=255)                                                                                                                   tATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgc          >  1:32926/1‑140 (MQ=255)                                                                                                                   tATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgc          >  1:979596/1‑140 (MQ=255)                                                                                                                    aTGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcg         <  2:44642/140‑1 (MQ=255)                                                                                                                    aTGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcg         <  2:324899/140‑1 (MQ=255)                                                                                                                    aTGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcg         <  1:983473/140‑1 (MQ=255)                                                                                                                    aTGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcg         <  2:930271/140‑1 (MQ=255)                                                                                                                    aTGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcg         <  1:852447/140‑1 (MQ=255)                                                                                                                     tGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACTGgcgcgc        <  2:285726/140‑1 (MQ=255)                                                                                                                     tGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc        <  1:209285/140‑1 (MQ=255)                                                                                                                     tGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc        <  2:744657/140‑1 (MQ=255)                                                                                                                     tGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc        <  1:644523/140‑1 (MQ=255)                                                                                                                     tGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc        <  1:11556/140‑1 (MQ=255)                                                                                                                          tCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGc   >  1:769903/1‑140 (MQ=255)                                                                                                                           cGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATGGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCt  <  1:1022578/140‑1 (MQ=255)                                                                                                                           cGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCt  <  1:957906/140‑1 (MQ=255)                                                                                                                           cGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCt  <  1:338149/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                              GGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCT  >  NC_000913/927072‑927333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑