Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	975,711	A→G	L30L (TTA→TTG)	mukE →	chromosome partitioning protein MukE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	975,711	0	A	G	100.0%	198.5 / NA	53	L30L (TTA→TTG)	mukE	chromosome partitioning protein MukE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/37);  total (16/37)

GCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGTTCTTCTATT  >  NC_000913/975576‑975844                                                                                                                                        |                                                                                                                                      gCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGtt                                                                                                                                   <  1:104996/140‑1 (MQ=255)   gAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCa                                                                                                                                 <  2:650180/140‑1 (MQ=255)   gAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCa                                                                                                                                 <  1:24191/140‑1 (MQ=255)      aTGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTGCAGGa                                                                                                                              <  2:424956/140‑1 (MQ=255)                  tAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGc                                                                                                                  <  2:284433/140‑1 (MQ=255)                       aTTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTcga                                                                                                             >  1:645275/1‑140 (MQ=255)                         tACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTcgacg                                                                                                           <  2:786470/140‑1 (MQ=255)                         tACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTcgacg                                                                                                           <  1:621497/140‑1 (MQ=255)                           cGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGaa                                                                                                         <  2:146320/140‑1 (MQ=255)                               gCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTgg                                                                                                     >  2:39937/1‑140 (MQ=255)                               gCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTgg                                                                                                     >  2:770647/1‑140 (MQ=255)                                ccAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGa                                                                                                    <  2:698306/140‑1 (MQ=255)                                   aGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATaa                                                                                                 >  2:702076/1‑140 (MQ=255)                                          ggCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCa                                                                                          <  1:302765/140‑1 (MQ=255)                                          ggCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCa                                                                                          >  2:664885/1‑140 (MQ=255)                                            cgcATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCAtt                                                                                        >  1:892620/1‑140 (MQ=255)                                              cATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTcc                                                                                      >  2:677952/1‑140 (MQ=255)                                                tGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTg                                                                                    >  2:454661/1‑140 (MQ=255)                                                tGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTg                                                                                    >  2:373803/1‑140 (MQ=255)                                                tGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTg                                                                                    >  1:939631/1‑140 (MQ=255)                                                tGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTg                                                                                    >  2:140667/1‑140 (MQ=255)                                                        aCAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGAttt                                                                            <  2:198675/140‑1 (MQ=255)                                                          aaaTATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTc                                                                          <  2:21024/140‑1 (MQ=255)                                                              atTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGa                                                                      <  1:220163/140‑1 (MQ=255)                                                                       gTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGa                                                             >  1:426971/1‑140 (MQ=255)                                                                        tGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGaa                                                            <  2:849730/140‑1 (MQ=255)                                                                            gCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGt                                                        >  2:549096/1‑140 (MQ=255)                                                                               ggTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGtttt                                                     <  2:210998/140‑1 (MQ=255)                                                                                 ttAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAc                                                   <  1:756278/140‑1 (MQ=255)                                                                                  tAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcg                                                  <  1:236773/140‑1 (MQ=255)                                                                                  tAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcg                                                  <  1:454661/140‑1 (MQ=255)                                                                                   aaGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcgc                                                 <  1:140667/140‑1 (MQ=255)                                                                                    aGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcgcg                                                <  2:645275/140‑1 (MQ=255)                                                                                    aGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcgcg                                                <  2:822558/140‑1 (MQ=255)                                                                                    aGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcgcg                                                <  2:945743/140‑1 (MQ=255)                                                                                    aGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcgcg                                                <  1:1007015/140‑1 (MQ=255)                                                                                    aGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcgcg                                                <  1:635310/140‑1 (MQ=255)                                                                                         gtgcAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTta                                           <  2:561929/138‑1 (MQ=255)                                                                                         gcgcAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTta                                           <  1:770647/140‑1 (MQ=255)                                                                                         gcgcAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTta                                           <  2:892620/140‑1 (MQ=255)                                                                                         gcgcAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTta                                           <  1:39937/140‑1 (MQ=255)                                                                                         gcgcAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTta                                           <  1:780402/140‑1 (MQ=255)                                                                                          cgcAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTtat                                          <  1:677952/140‑1 (MQ=255)                                                                                              ggCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACg                                      >  2:106509/1‑140 (MQ=255)                                                                                               gcgcTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGt                                     >  1:868684/1‑140 (MQ=255)                                                                                                           ccGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTcg                         <  1:867737/140‑1 (MQ=255)                                                                                                           ccGTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTcg                         <  2:616808/140‑1 (MQ=255)                                                                                                             gTTATTTCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTcgcg                       <  2:680349/140‑1 (MQ=255)                                                                                                                   tCCGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAg                 <  1:549096/140‑1 (MQ=255)                                                                                                                     cGGCGCTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGaa               <  1:373803/140‑1 (MQ=255)                                                                                                                        cgcTGGACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAggg            <  2:929477/140‑1 (MQ=255)                                                                                                                             gACAGCGCCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGttctt       <  1:664885/140‑1 (MQ=255)                                                                                                                                  cgcCTTGCGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGTTCTTCTAtt  >  2:363242/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      GCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGTTCTTCTATT  >  NC_000913/975576‑975844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑