Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	986,448	A→G	Y179H (TAC→CAC)	ompF ←	outer membrane porin F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	986,448	0	A	G	100.0%	269.0 / NA	73	Y179H (TAC→CAC)	ompF	outer membrane porin F
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/43);  total (30/43)

TTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTG  >  NC_000913/986314‑986584                                                                                                                                       |                                                                                                                                         ttcttcCTGGTTGGTACGGTCACCTGCACCATAAGCACAACCGATCCCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGaa                                                                                                                                     <  2:1036961/140‑1 (MQ=255) ttctgGCAGGTTGGTCCGGTCAGCGGCACCATAAGCCCCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGaa                                                                                                                                     <  2:154190/140‑1 (MQ=255)     tGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGc                                                                                                                                 >  2:386560/1‑140 (MQ=255)             ggTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCa                                                                                                                         <  1:808062/140‑1 (MQ=255)             ggTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCa                                                                                                                         <  1:386705/140‑1 (MQ=255)              gTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAg                                                                                                                        >  1:894553/1‑140 (MQ=255)                 cGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGcc                                                                                                                     <  1:30676/140‑1 (MQ=255)                  ggTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCa                                                                                                                    <  2:664690/140‑1 (MQ=255)                  ggTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCa                                                                                                                    <  1:300214/140‑1 (MQ=255)                    tCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATc                                                                                                                  >  2:998642/1‑140 (MQ=255)                     cAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCa                                                                                                                 >  1:233729/1‑140 (MQ=255)                      aGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCaa                                                                                                                >  2:13535/1‑140 (MQ=255)                      aGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCaa                                                                                                                >  1:53061/1‑140 (MQ=255)                           cACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGa                                                                                                           >  2:260027/1‑140 (MQ=255)                                     aCCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGt                                                                                                 >  2:141007/1‑140 (MQ=255)                                     aCCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGt                                                                                                 >  2:857749/1‑140 (MQ=255)                                     aCCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGt                                                                                                 >  1:961384/1‑140 (MQ=255)                                     aCCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGt                                                                                                 >  2:263023/1‑140 (MQ=255)                                     aCCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGt                                                                                                 >  2:810688/1‑140 (MQ=255)                                      ccAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGtt                                                                                                <  2:196750/140‑1 (MQ=255)                                        aaCGATCCCAAGGCCTCCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTgg                                                                                              <  2:89228/140‑1 (MQ=255)                                        aaCGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTgg                                                                                              >  1:4871/1‑140 (MQ=255)                                        aaCGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTgg                                                                                              >  1:56055/1‑140 (MQ=255)                                        aaCGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTgg                                                                                              >  2:617054/1‑140 (MQ=255)                                         aCGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGa                                                                                             <  2:499521/140‑1 (MQ=255)                                          cGATACCAAAGCCTTCGTATTTGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAg                                                                                            <  1:803539/140‑1 (MQ=255)                                          cGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAg                                                                                            <  2:228353/140‑1 (MQ=255)                                             tACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTa                                                                                         <  2:327182/140‑1 (MQ=255)                                               ccAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACg                                                                                       <  1:211861/140‑1 (MQ=255)                                               ccAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACg                                                                                       <  1:460021/140‑1 (MQ=255)                                                cAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGa                                                                                      <  2:56055/140‑1 (MQ=255)                                                 aaaGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGGTACGAt                                                                                     >  1:407253/1‑140 (MQ=255)                                                     ccTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGt                                                                                 >  2:264004/1‑140 (MQ=255)                                                       ttcgtattcgtaGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAg                                                                               >  1:826812/1‑140 (MQ=255)                                                       ttcgtattcgtaGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAg                                                                               >  1:417202/1‑140 (MQ=255)                                                        tcgtattcgtaGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGGAGc                                                                              >  1:372428/1‑140 (MQ=255)                                                         cgtattcgtaGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCa                                                                             >  2:216961/1‑140 (MQ=255)                                                          gtattcgtaGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCaa                                                                            <  1:887229/140‑1 (MQ=255)                                                                 taGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGCGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACgccgcc                                                                     >  2:416562/1‑140 (MQ=255)                                                                   gCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCaa                                                                   >  1:249387/1‑140 (MQ=255)                                                                   gCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCaa                                                                   <  2:826812/140‑1 (MQ=255)                                                                    cTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAac                                                                  >  1:348851/1‑140 (MQ=255)                                                                        tAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGa                                                              <  1:742975/140‑1 (MQ=255)                                                                            aCCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCaa                                                          >  1:1030032/1‑140 (MQ=255)                                                                                ccAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACgaa                                                      >  1:569934/1‑140 (MQ=255)                                                                                  aaCACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACgaaga                                                    >  2:356044/1‑140 (MQ=255)                                                                                     acCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGt                                                 <  2:4871/140‑1 (MQ=255)                                                                                       cGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCa                                               <  1:141007/140‑1 (MQ=255)                                                                                       cGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCa                                               <  1:490247/140‑1 (MQ=255)                                                                                       cGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCa                                               <  1:857749/140‑1 (MQ=255)                                                                                       cGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCa                                               <  2:491710/140‑1 (MQ=255)                                                                                          cGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCg                                            <  1:478706/140‑1 (MQ=255)                                                                                          cGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCg                                            <  1:810688/140‑1 (MQ=255)                                                                                            ccGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCt                                          <  1:812860/140‑1 (MQ=255)                                                                                            ccGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCt                                          <  2:53061/140‑1 (MQ=255)                                                                                             cGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTg                                         <  1:869629/140‑1 (MQ=255)                                                                                               ttAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTa                                       <  1:263023/140‑1 (MQ=255)                                                                                               ttAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTa                                       <  2:894553/140‑1 (MQ=255)                                                                                               ttAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTa                                       <  1:245908/140‑1 (MQ=255)                                                                                                tAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTAt                                      <  2:348851/140‑1 (MQ=255)                                                                                                   aaCGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCa                                   >  1:261717/1‑140 (MQ=255)                                                                                                   aaCGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCa                                   <  1:998642/140‑1 (MQ=255)                                                                                                                    cGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTc                  >  1:962565/1‑140 (MQ=255)                                                                                                                    cGCTCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTc                  <  2:961384/140‑1 (MQ=255)                                                                                                                       tCGTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTgg               <  1:260027/140‑1 (MQ=255)                                                                                                                         gTTTTTACCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgca             >  2:853104/1‑140 (MQ=255)                                                                                                                               aCCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCatat       <  1:628200/140‑1 (MQ=255)                                                                                                                               aCCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCatat       <  1:617054/140‑1 (MQ=255)                                                                                                                               aCCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCatat       <  2:544704/140‑1 (MQ=255)                                                                                                                               aCCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCatat       <  2:326892/140‑1 (MQ=255)                                                                                                                               aCCCAGGTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCatat       <  1:216961/140‑1 (MQ=255)                                                                                                                                    ggTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGgtg  <  2:233729/140‑1 (MQ=255)                                                                                                                                    ggTGCTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGgtg  <  1:416562/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         TTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGGCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTG  >  NC_000913/986314‑986584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑