Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,040,030	A→G	K245K (AAA→AAG)	appB →	cytochrome bd‑II ubiquinol oxidase subunit II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,040,030	0	A	G	100.0%	162.0 / NA	44	K245K (AAA→AAG)	appB	cytochrome bd‑II ubiquinol oxidase subunit II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/18);  total (26/18)

AGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGG  >  NC_000913/1039897‑1040163                                                                                                                                      |                                                                                                                                      ggCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCACAGGATGATAACGGACCTTCCAATCCGTTAATGAAGCtggtg                                                                                                                                 <  2:406963/139‑1 (MQ=255) aGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCtggtg                                                                                                                                 <  2:661849/140‑1 (MQ=255)   cTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGc                                                                                                                               <  1:873216/140‑1 (MQ=255)    tGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCa                                                                                                                              <  1:823214/140‑1 (MQ=255)       cGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTg                                                                                                                           <  1:279683/140‑1 (MQ=255)         aCCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCt                                                                                                                         <  2:168956/140‑1 (MQ=255)                     gCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTg                                                                                                             <  2:833917/140‑1 (MQ=255)                      cACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTgg                                                                                                            <  2:609100/140‑1 (MQ=255)                           ttGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGGCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGGGCCTGGATGaa                                                                                                       >  2:415845/1‑140 (MQ=255)                                   gCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGGCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGGGCCTGGATGAATAATTTTg                                                                                               >  1:48652/1‑140 (MQ=255)                                     tgtgCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTc                                                                                             >  1:952904/1‑140 (MQ=255)                                     tgtgCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGGGCCTGGATGAATAATTTTGTc                                                                                             >  2:341494/1‑140 (MQ=255)                                     tgtgCTTTTTGCTGGCGGGTTACTGGCTGTGGGGCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTc                                                                                             >  2:417528/1‑140 (MQ=255)                                     tgtgCTTTTTGCTGGCGGGTTACTGGCTGTGGGGCGGGATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCCGTGCTACCTGGGGCCTGGATGAATAATTTTGTc                                                                                             >  2:108657/1‑140 (MQ=255)                                               gCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCg                                                                                   <  2:278877/140‑1 (MQ=255)                                               gCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCg                                                                                   <  2:962033/140‑1 (MQ=255)                                                  ggCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGttt                                                                                <  1:411282/140‑1 (MQ=255)                                                                tgtgGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCg                                                                  <  2:818833/140‑1 (MQ=255)                                                                tgtgGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCg                                                                  <  2:333837/140‑1 (MQ=255)                                                                         gTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGa                                                         <  1:922063/140‑1 (MQ=255)                                                                         gTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGa                                                         <  2:554365/140‑1 (MQ=255)                                                                           aTTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGAtt                                                       <  2:1014495/140‑1 (MQ=255)                                                                               aTGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGAttcttc                                                   >  2:206667/1‑140 (MQ=255)                                                                               aTGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGAttcttc                                                   >  2:495167/1‑140 (MQ=255)                                                                                    tttGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGccc                                              >  2:888345/1‑140 (MQ=255)                                                                                    tttGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGccc                                              >  1:419729/1‑140 (MQ=255)                                                                                    tttGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTAGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGccc                                              >  2:493124/1‑140 (MQ=255)                                                                                      tGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCAt                                            >  2:734962/1‑140 (MQ=255)                                                                                          cTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCt                                        <  1:417528/140‑1 (MQ=255)                                                                                             cTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGTTTCTTCTGCCCATTGCTGAc                                     >  1:22379/1‑140 (MQ=255)                                                                                             cTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGAc                                     >  2:182110/1‑140 (MQ=255)                                                                                             cTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGAc                                     >  1:997722/1‑140 (MQ=255)                                                                                             cTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGAc                                     >  1:808638/1‑140 (MQ=255)                                                                                             cTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGAc                                     >  1:448780/1‑140 (MQ=255)                                                                                             cTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGAc                                     >  2:428523/1‑140 (MQ=255)                                                                                             cTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGAc                                     >  2:43478/1‑140 (MQ=255)                                                                                                  ccAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGa                                >  1:256925/1‑140 (MQ=255)                                                                                                  ccAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGa                                >  1:788367/1‑140 (MQ=255)                                                                                                     ggATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATgg                             >  2:941916/1‑140 (MQ=255)                                                                                                                gTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGt                  >  2:190285/1‑140 (MQ=255)                                                                                                                gTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGt                  >  1:581102/1‑140 (MQ=255)                                                                                                                   cTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGt               <  1:403882/140‑1 (MQ=255)                                                                                                                             gTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGCCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGtt     >  2:906626/1‑140 (MQ=255)                                                                                                                                aaTGAAGTTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTggg  >  1:441663/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                      AGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGG  >  NC_000913/1039897‑1040163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑