Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	92,082	A→G	T224A (ACT→GCT)	ftsI →	peptidoglycan DD‑transpeptidase FtsI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	92,082	0	A	G	100.0%	201.0 / NA	54	T224A (ACT→GCT)	ftsI	peptidoglycan DD‑transpeptidase FtsI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/25);  total (29/25)

TCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACA  >  NC_000913/91945‑92217                                                                                                                                          |                                                                                                                                        caaTCGGGTTTCCTATCGTCGATAGTCAAGGGATTGAGGGCTTTGAGAAGAGTTTCGATAAATGGCTTACCGGTGTGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCt                                                                                                                                       <  2:146894/138‑1 (MQ=255)   aTCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGa                                                                                                                                     <  2:1035874/140‑1 (MQ=255)   aTCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGa                                                                                                                                     >  1:310722/1‑140 (MQ=255)     cGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACa                                                                                                                                   >  2:106739/1‑140 (MQ=255)          ttACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAg                                                                                                                              >  1:371995/1‑140 (MQ=255)           tACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAgg                                                                                                                             <  2:773191/140‑1 (MQ=255)                  gTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGca                                                                                                                      >  2:945273/1‑140 (MQ=255)                  gTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGca                                                                                                                      >  2:424741/1‑140 (MQ=255)                  gTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGca                                                                                                                      >  1:429032/1‑140 (MQ=255)                  gTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGca                                                                                                                      <  2:499912/140‑1 (MQ=255)                       tAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAAcc                                                                                                                 >  2:970771/1‑140 (MQ=255)                         gTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTg                                                                                                               <  2:493311/140‑1 (MQ=255)                            aaGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGgcg                                                                                                            <  2:867401/140‑1 (MQ=255)                            aaGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGgcg                                                                                                            >  1:993753/1‑140 (MQ=255)                              gggATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCt                                                                                                          <  2:29148/140‑1 (MQ=255)                              gggATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCt                                                                                                          <  1:840599/140‑1 (MQ=255)                              gggATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCt                                                                                                          <  1:8461/140‑1 (MQ=255)                                 aTTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAg                                                                                                       >  2:767342/1‑140 (MQ=255)                                 aTTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAg                                                                                                       >  2:661330/1‑140 (MQ=255)                                   tGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTa                                                                                                     >  2:564340/1‑140 (MQ=255)                                    gAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTAt                                                                                                    >  1:905885/1‑140 (MQ=255)                                    gAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTAt                                                                                                    <  2:229763/140‑1 (MQ=255)                                    gAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTAt                                                                                                    >  1:758562/1‑140 (MQ=255)                                            tGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGAGAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAAc                                                                                            >  1:866455/1‑140 (MQ=255)                                            tGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAAc                                                                                            >  2:1512/1‑140 (MQ=255)                                                     tttCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAgg                                                                                   <  1:843516/140‑1 (MQ=255)                                                       tCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGgcg                                                                                 >  2:641552/1‑140 (MQ=255)                                                                           cAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGaa                                                             >  1:1032966/1‑140 (MQ=255)                                                                                   tGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCgg                                                     <  2:592115/140‑1 (MQ=255)                                                                                   tGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCgg                                                     <  2:102204/140‑1 (MQ=255)                                                                                             gtgCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTaa                                           <  1:661330/140‑1 (MQ=255)                                                                                              tgCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAAc                                          <  1:564340/140‑1 (MQ=255)                                                                                              tgCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAAc                                          <  1:808684/140‑1 (MQ=255)                                                                                               gCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACa                                         >  1:761240/1‑140 (MQ=255)                                                                                                  tAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAgg                                      <  1:426286/140‑1 (MQ=255)                                                                                                  tAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAgg                                      <  2:310722/140‑1 (MQ=255)                                                                                                       aCCGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGaa                                 <  1:73900/140‑1 (MQ=255)                                                                                                        ccGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAAt                                >  1:207150/1‑140 (MQ=255)                                                                                                        ccGCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAAt                                >  2:515928/1‑140 (MQ=255)                                                                                                        ccGCTATGGTCGCGTAATTGAAGACATTTATTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCAGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAAt                                >  2:597077/1‑140 (MQ=255)                                                                                                          gCTATGGTCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCt                              >  2:140002/1‑140 (MQ=255)                                                                                                                 tCGCGTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAgcg                       <  2:535885/140‑1 (MQ=255)                                                                                                                  cgcgTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAgcgc                      <  1:945273/140‑1 (MQ=255)                                                                                                                   gcgTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGcc                     >  1:5505/1‑140 (MQ=255)                                                                                                                    cgTAATTGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCg                    >  1:945286/1‑140 (MQ=255)                                                                                                                          tGAAGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCtgg              >  1:22431/1‑140 (MQ=255)                                                                                                                            aaGATATTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCtggtg            <  1:970771/140‑1 (MQ=255)                                                                                                                                 atTTCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGCATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGt       >  1:371888/1‑140 (MQ=255)                                                                                                                                  tttCTTCTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTCATGAGCGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTc      <  2:1032083/140‑1 (MQ=255)                                                                                                                                   ttcttcTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCa     >  2:512962/1‑140 (MQ=255)                                                                                                                                   ttcttcTGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCa     >  1:109753/1‑140 (MQ=255)                                                                                                                                    tcttctGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCaa    <  1:424741/140‑1 (MQ=255)                                                                                                                                      ttctGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAaca  <  1:767342/140‑1 (MQ=255)                                                                                                                                      ttctGCTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAaca  >  2:289604/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        TCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACA  >  NC_000913/91945‑92217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑