Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,259,759	A→G	S349P (TCC→CCC)	dauA ←	aerobic C4‑dicarboxylate transporter DauA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,259,759	0	A	G	100.0%	164.6 / NA	46	S349P (TCC→CCC)	dauA	aerobic C4‑dicarboxylate transporter DauA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/20);  total (26/20)

TTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCG  >  NC_000913/1259628‑1259892                                                                                                                                    |                                                                                                                                      ttCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGccc                                                                                                                               >  2:36076/1‑140 (MQ=255)  tCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGcccc                                                                                                                              >  1:504131/1‑140 (MQ=255)        aTGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACg                                                                                                                        <  2:453268/140‑1 (MQ=255)           ttCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGAc                                                                                                                     >  1:873295/1‑140 (MQ=255)              cACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGtt                                                                                                                  >  1:911358/1‑140 (MQ=255)                cGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAg                                                                                                                >  1:108565/1‑140 (MQ=255)                cGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAg                                                                                                                >  2:420652/1‑140 (MQ=255)                    accaTCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGc                                                                                                            >  1:356275/1‑140 (MQ=255)                    accaTCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGc                                                                                                            >  2:179413/1‑140 (MQ=255)                               cagGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGa                                                                                                 >  1:337307/1‑140 (MQ=255)                               cagGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGa                                                                                                 >  1:1028673/1‑140 (MQ=255)                                  ggCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATgg                                                                                              <  1:460148/140‑1 (MQ=255)                                       ccATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCt                                                                                         <  1:449137/140‑1 (MQ=255)                                         aTGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGTCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGt                                                                                       <  2:514688/140‑1 (MQ=255)                                            gCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGc                                                                                    >  2:380802/1‑140 (MQ=255)                                            gCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGc                                                                                    >  1:536049/1‑140 (MQ=255)                                             cGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCg                                                                                   <  2:979864/140‑1 (MQ=255)                                                      gCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATacca                                                                          >  2:834474/1‑140 (MQ=255)                                                      gCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATacca                                                                          <  1:543043/140‑1 (MQ=255)                                                              gggagCAGCGGTGCCAGTCCCAGCAGGGCAAGAATAACCAGAATAGAGGGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGaa                                                                  <  2:93917/138‑1 (MQ=255)                                                               agagCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAAc                                                                 <  2:122997/140‑1 (MQ=255)                                                                gagCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACg                                                                <  1:420652/140‑1 (MQ=255)                                                                   cagcGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAg                                                             >  2:923782/1‑140 (MQ=255)                                                                   cagcGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAg                                                             <  2:289772/140‑1 (MQ=255)                                                                   cagcGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAg                                                             <  2:710459/140‑1 (MQ=255)                                                                   cagcGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGCGGGCGCTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAg                                                             >  2:580940/1‑140 (MQ=255)                                                                      cGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGa                                                          >  1:971353/1‑140 (MQ=255)                                                                           ccAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGataata                                                     <  2:945908/140‑1 (MQ=255)                                                                           ccAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGataata                                                     <  1:852151/140‑1 (MQ=255)                                                                                aCCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTccc                                                >  1:913471/1‑140 (MQ=255)                                                                                     cagGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTc                                           <  1:179413/140‑1 (MQ=255)                                                                                     cagGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTc                                           >  2:780421/1‑140 (MQ=255)                                                                                     cagGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTc                                           <  2:1028673/140‑1 (MQ=255)                                                                                     cagGGCAAGAAAAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTc                                           <  1:111870/140‑1 (MQ=255)                                                                                       gggCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGTCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTccc                                         <  2:754355/140‑1 (MQ=255)                                                                                                  aCCAGAATAGAGTGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAg                              >  1:411172/1‑140 (MQ=255)                                                                                                              tGGATCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGc                  >  1:304331/1‑140 (MQ=255)                                                                                                                 aTCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCtt               >  2:515776/1‑140 (MQ=255)                                                                                                                 aTCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCtt               >  2:597081/1‑140 (MQ=255)                                                                                                                 aTCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCtt               >  2:715880/1‑140 (MQ=255)                                                                                                                 aTCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCtt               >  1:559695/1‑140 (MQ=255)                                                                                                                 aTCACCGCCGAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCtt               <  2:108565/140‑1 (MQ=255)                                                                                                                      cgccgAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGtt          >  2:496432/1‑140 (MQ=255)                                                                                                                      cgccgAGATAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATCGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGtt          >  2:771106/1‑140 (MQ=255)                                                                                                                           agaTAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGTACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTc     <  2:820899/140‑1 (MQ=255)                                                                                                                              tAGGGGGCGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCg  <  1:988175/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      TTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCG  >  NC_000913/1259628‑1259892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑