Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,287,842	T→C	A261A (GCA→GCG)	purU ←	formyltetrahydrofolate deformylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,842	0	T	C	100.0%	223.4 / NA	60	A261A (GCA→GCG)	purU	formyltetrahydrofolate deformylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/39);  total (21/39)

TACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAG  >  NC_000913/1287705‑1287975                                                                                                                                          |                                                                                                                                      tACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAgcgc                                                                                                                                     >  1:292883/1‑140 (MQ=255) tACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAgcgc                                                                                                                                     >  2:533064/1‑140 (MQ=255)   cGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCAc                                                                                                                                   >  1:61626/1‑140 (MQ=255)   cGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCAc                                                                                                                                   >  1:555986/1‑140 (MQ=255)       gagaAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACt                                                                                                                               <  1:924812/140‑1 (MQ=255)        agaAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACtt                                                                                                                              >  2:255256/1‑140 (MQ=255)                  aCTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGtttt                                                                                                                    >  2:541154/1‑140 (MQ=255)                     ggTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTtct                                                                                                                 <  2:458560/140‑1 (MQ=255)                     ggTTGCGTAATTTTCATCCGTAACAGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTtct                                                                                                                 >  2:710845/1‑140 (MQ=255)                       ttGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCg                                                                                                               <  1:635622/140‑1 (MQ=255)                       ttGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCg                                                                                                               <  1:282545/140‑1 (MQ=255)                                    aTCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTg                                                                                                  >  1:846668/1‑140 (MQ=255)                                      ccGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTgcg                                                                                                <  2:829498/140‑1 (MQ=255)                                      ccGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTgcg                                                                                                <  2:958606/140‑1 (MQ=255)                                      ccGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTgcg                                                                                                <  1:455269/140‑1 (MQ=255)                                            cGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGcatc                                                                                          <  2:655157/140‑1 (MQ=255)                                            cGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGcatc                                                                                          <  1:234707/140‑1 (MQ=255)                                                   aaGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCtt                                                                                   >  2:504466/1‑140 (MQ=255)                                                    aGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTc                                                                                  <  2:888490/140‑1 (MQ=255)                                                      gTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                                                <  2:590636/140‑1 (MQ=255)                                                      gTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                                                <  2:1009475/140‑1 (MQ=255)                                                      gTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                                                <  1:929749/140‑1 (MQ=255)                                                      gTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                                                <  2:632928/140‑1 (MQ=255)                                                      gTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                                                <  2:673122/140‑1 (MQ=255)                                                      gTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                                                <  2:901184/140‑1 (MQ=255)                                                      gTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                                                <  1:250045/140‑1 (MQ=255)                                                      gTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                                                <  2:950548/140‑1 (MQ=255)                                                      gTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                                                <  1:555539/140‑1 (MQ=255)                                                       tAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGc                                                                               <  2:470166/140‑1 (MQ=255)                                                        aaCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCt                                                                              <  1:814964/140‑1 (MQ=255)                                                        aaCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCt                                                                              >  2:841021/1‑140 (MQ=255)                                                        aaCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCt                                                                              >  2:958402/1‑140 (MQ=255)                                                         aCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCtg                                                                             >  1:947186/1‑140 (MQ=255)                                                         aCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCtg                                                                             >  2:767606/1‑140 (MQ=255)                                                          ccAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCtgt                                                                            <  1:376838/140‑1 (MQ=255)                                                          ccAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCtgt                                                                            <  2:292883/140‑1 (MQ=255)                                                             gTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTa                                                                         >  2:448870/1‑140 (MQ=255)                                                               tATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAgg                                                                       >  2:192360/1‑140 (MQ=255)                                                                    ttGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGa                                                                  <  2:712760/140‑1 (MQ=255)                                                                     tGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGAt                                                                 <  1:260643/140‑1 (MQ=255)                                                                      gCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATc                                                                <  1:682774/140‑1 (MQ=255)                                                                         ggCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGAc                                                             <  1:710845/140‑1 (MQ=255)                                                                                     ataatCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTc                                                 <  1:533064/140‑1 (MQ=255)                                                                                     ataatCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTc                                                 <  1:208578/140‑1 (MQ=255)                                                                                            ttCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCatg                                          <  2:555986/140‑1 (MQ=255)                                                                                            ttCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCatg                                          >  2:850617/1‑140 (MQ=255)                                                                                            ttCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCatg                                          >  1:1017934/1‑140 (MQ=255)                                                                                                   aCCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTg                                   <  1:818057/140‑1 (MQ=255)                                                                                                     cGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTggg                                 <  1:133149/140‑1 (MQ=255)                                                                                                       tAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGcc                               <  1:448870/140‑1 (MQ=255)                                                                                                         aaCAAAGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCtt                             <  1:95489/140‑1 (MQ=255)                                                                                                            aaaGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGt                          <  1:928911/140‑1 (MQ=255)                                                                                                             aaGACGCGCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGCCCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTc                         <  2:145514/140‑1 (MQ=255)                                                                                                              aGACGCGCTGTGCCAGCACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTcc                        >  1:493264/1‑140 (MQ=255)                                                                                                                 cgcgCTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGa                     <  2:575721/140‑1 (MQ=255)                                                                                                                   cgcTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGAtt                   >  2:731978/1‑140 (MQ=255)                                                                                                                    gcTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTg                  >  1:596606/1‑140 (MQ=255)                                                                                                                     cTGTGCCAGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGt                 <  1:767606/140‑1 (MQ=255)                                                                                                                            aGTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTcac          <  2:1022088/140‑1 (MQ=255)                                                                                                                             gTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTcaca         <  2:50733/140‑1 (MQ=255)                                                                                                                             gTACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTcaca         >  2:828032/1‑140 (MQ=255)                                                                                                                              tACTTTGTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACAt        >  2:216743/1‑140 (MQ=255)                                                                                                                                    gTATAGCGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAg  >  2:956542/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      TACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAG  >  NC_000913/1287705‑1287975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑