Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,436,241	T→C	Y218C (TAC→TGC)	ompN ←	outer membrane porin N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,436,241	0	T	C	100.0%	251.7 / NA	67	Y218C (TAC→TGC)	ompN	outer membrane porin N
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/30);  total (37/30)

TATTACGCGTTTCTGAATACATGGTTGCCAGGTAAATATTGTTAGCATCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGTATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTGACCATTACTGGCTCCTTCGTTGTTACCT  >  NC_000913/1436104‑1436375                                                                                                                                          |                                                                                                                                       tatTACGCGTTTCTGAATACATGGTTGCCAGGTAAATATTGTTAGCATCGCATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCAt                                                                                                                                      >  2:460619/1‑140 (MQ=255)            tCTGAATACATGGTTGCCAGGTAAATATTGTTAGCATCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAgc                                                                                                                           >  1:755344/1‑140 (MQ=255)            tCTGAATACATGGTTGCCAGGTAAATATTGTTAGCATCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAgc                                                                                                                           >  1:491817/1‑140 (MQ=255)            tCTGAATACATGGTTGCCAGGTAAATATTGTTAGCATCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAgc                                                                                                                           >  1:393001/1‑140 (MQ=255)                aaTACATGGTTGCCAGGTAAATATTGTTAGCATCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTa                                                                                                                       >  2:241516/1‑140 (MQ=255)                     aTGGTTGCCAGGTAAATATTGTTAGCATCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGcc                                                                                                                  >  2:333570/1‑140 (MQ=255)                     aTGGTTGCCAGGTAAATATTGTTAGCATCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGcc                                                                                                                  >  1:479092/1‑140 (MQ=255)                       ggTTGCCAGGTAAATTTTGTTAGCATCGTATTTTAGCCCAGCAGCCCCCGCGTCTGCTTTATCCCCCCCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCa                                                                                                                <  2:19107/140‑1 (MQ=255)                       ggTTGCCAGGTAAATATTGTTAGCATCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCa                                                                                                                >  1:987432/1‑140 (MQ=255)                       ggTTGCCAGGTAAATATTGTTAGCATCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCa                                                                                                                >  1:531808/1‑140 (MQ=255)                          tGCCAGGTAAATATTGTTAGCATCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGc                                                                                                             <  2:993555/140‑1 (MQ=255)                            ccgggTAAATATTGTTAGCCTCGTTTTTTACCCCAGCAGCCCACGCGTCTGCTTTACCCCCCCCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCt                                                                                                           <  2:668600/137‑1 (MQ=255)                            ccagtaaaaTATTTTTAGCATCTTTTTTTAGCCCAGCAGCCCACGCGTCTGTTTTATCCCCCCCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCt                                                                                                           <  2:487232/134‑1 (MQ=255)                            ccAGGTAAATTTTGTTAGCATCGTATTTTAGCCCAGCAGCCCCCGCGTCTGTTTTACCACCCCCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCt                                                                                                           <  2:1008887/140‑1 (MQ=255)                            ccAGGTAAATATTGTTAGCATCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCCCCCCCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCt                                                                                                           <  1:276612/140‑1 (MQ=255)                            ccAGGTAAATATTGTTAGCATCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCCCCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCt                                                                                                           <  1:585510/140‑1 (MQ=255)                            ccAGGTAAATATTGTTAGCATCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCt                                                                                                           >  1:89378/1‑140 (MQ=255)                            ccAGGTAAATATTGTTAGCATCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCt                                                                                                           >  1:437218/1‑140 (MQ=255)                                   aaTATTGTTAGCATCGTTTTTTAGCCCAGCGGTCCCCGCGTCTGCTTTACCCCCCCCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCAAGCCGCACCAGCGCTAAAGCCCATGCCTAAATCat                                                                                                    <  2:697631/140‑1 (MQ=255)                                   aaTATTGTTAGCATCGTTTTTTAGCCCAGCAGTCCCCGCGTCTGTTTTATCACCCCCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCat                                                                                                    <  1:268558/140‑1 (MQ=255)                                   aaTATTGTTAGCATCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCat                                                                                                    <  1:357109/140‑1 (MQ=255)                                      tttGTTAGCATCGTTTTTTGGCCCGGCAGCCCCCGCGTCTGCTTTATCCCCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATAtg                                                                                                 <  2:415519/139‑1 (MQ=255)                                         gTTAGCATCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATAtgttg                                                                                              >  2:407055/1‑140 (MQ=255)                                         gTTAGCATCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATAtgttg                                                                                              >  1:573637/1‑140 (MQ=255)                                           tAGCATCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAATCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTtgtg                                                                                            >  2:1011531/1‑140 (MQ=255)                                               aTCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGaaa                                                                                        >  2:346519/1‑140 (MQ=255)                                               aTCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGaaa                                                                                        >  1:335358/1‑140 (MQ=255)                                               aTCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGaaa                                                                                        >  2:800475/1‑140 (MQ=255)                                                 cGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGa                                                                                      >  2:870413/1‑140 (MQ=255)                                                     ttttACCCCACCAGTCCACGCTTCTCTTTTATCCCCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGAcccc                                                                                  <  1:182558/140‑1 (MQ=255)                                                            ccAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTc                                                                           <  1:241516/140‑1 (MQ=255)                                                             cagcagTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCa                                                                          >  2:452924/1‑140 (MQ=255)                                                                cagTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGTCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCg                                                                       >  2:642104/1‑140 (MQ=255)                                                                   tCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGttt                                                                    >  2:183298/1‑140 (MQ=255)                                                                    ccACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGGGCGGTCAGAAGAATCGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGtttt                                                                   >  1:312595/1‑140 (MQ=255)                                                                     cACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTc                                                                  >  1:240701/1‑140 (MQ=255)                                                                          gTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGc                                                             >  2:544227/1‑140 (MQ=255)                                                                           tCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCg                                                            <  1:870413/140‑1 (MQ=255)                                                                                tttATCCCCCCCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACAt                                                       <  1:37942/140‑1 (MQ=255)                                                                                    tCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACg                                                   <  1:800475/140‑1 (MQ=255)                                                                                         accCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCgt                                              >  2:1035222/1‑140 (MQ=255)                                                                                         accCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCgt                                              >  2:276112/1‑140 (MQ=255)                                                                                         accCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCgt                                              >  1:446613/1‑140 (MQ=255)                                                                                            cgccgcAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCCGCGCTAAAGCCCATGCCTAAATAATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCgttgt                                           <  2:312595/140‑1 (MQ=255)                                                                                                gcAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGTCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGt                                       <  1:642104/140‑1 (MQ=255)                                                                                                 cAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTg                                      <  1:793031/140‑1 (MQ=255)                                                                                                   gTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGcc                                    >  2:144753/1‑140 (MQ=255)                                                                                                      tGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAATATCACGTCCGTTGTTGGTGCCTTc                                 >  1:305982/1‑140 (MQ=255)                                                                                                         ttAACCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTg                              >  1:904418/1‑140 (MQ=255)                                                                                                           aaCCTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTGAc                            <  2:987432/140‑1 (MQ=255)                                                                                                              cTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTGACCAt                         >  2:802944/1‑140 (MQ=255)                                                                                                              cTGGTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTGACCAt                         >  1:238141/1‑140 (MQ=255)                                                                                                                 gTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTGACCATTAc                      >  2:92309/1‑140 (MQ=255)                                                                                                                 gTCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTGACCATTAc                      >  1:310113/1‑140 (MQ=255)                                                                                                                  tCATTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTGACCATTACt                     >  1:317310/1‑140 (MQ=255)                                                                                                                    aTTGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTGACCATTACTgg                   <  1:452924/140‑1 (MQ=255)                                                                                                                      tGGTGCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTGACCATTACTGGCt                 >  1:56808/1‑140 (MQ=255)                                                                                                                          gCGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTGACCATTACTGGCTCCtt             <  2:822798/140‑1 (MQ=255)                                                                                                                           cGGTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTGACCATTACTGGCTCCTTc            >  2:914752/1‑140 (MQ=255)                                                                                                                            ggTCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTGACCATTACTGGCTCCTTCg           <  2:335358/140‑1 (MQ=255)                                                                                                                              tCAGAAGAGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTGACCATTACTGGCTCCTTCgtt         <  2:437218/140‑1 (MQ=255)                                                                                                                                agaagaGGTGCATGCCGCGCCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGGCCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTGACCATTACTGGCTCCTTCgttgt       <  1:382074/140‑1 (MQ=255)                                                                                                                                agaagaGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTGACCATTACTGGCTCCTTCgttgt       <  1:1011531/140‑1 (MQ=255)                                                                                                                                agaagaGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTGACCATTACTGGCTCCTTCgttgt       <  2:491817/140‑1 (MQ=255)                                                                                                                                agaagaGGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTGACCATTACTGGCTCCTTCgttgt       <  2:393001/140‑1 (MQ=255)                                                                                                                                   agagGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTGACCATTACTGGCTCCTTCGTTGTTAc    <  1:460619/140‑1 (MQ=255)                                                                                                                                     agGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTGACCATTACTGGCTCCTTCGTTGTTACCt  <  2:446613/140‑1 (MQ=255)                                                                                                                                     agGTGCATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTGACCATTACTGGCTCCTTCGTTGTTACCt  <  1:913885/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       TATTACGCGTTTCTGAATACATGGTTGCCAGGTAAATATTGTTAGCATCGTATTTTAGCCCAGCAGTCCACGCGTCTGCTTTATCACCACCCGCCGCAGTATGGTTAACCTGGTCATTGGTGCGGTCAGAAGAGGTGTATGCCGCACCAGCGCTAAAGCCCATGCCTAAATCATATGTTGTGGAAAGACCCCAGCCGTCACCGTTTTCATGGCGAACATCACGTCCGTTGTTGGTGCCTTCCTGACCATTACTGGCTCCTTCGTTGTTACCT  >  NC_000913/1436104‑1436375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑