Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 97,308 T→C G74G (GGT→GGC)  murD → UDP‑N‑acetylmuramoyl‑L‑alanine‑‑D‑glutamate ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091397,3080TC98.2% 199.2 / ‑5.6 55G74G (GGT→GGC) murDUDP‑N‑acetylmuramoyl‑L‑alanine‑‑D‑glutamate ligase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (31/23);  total (32/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGACGCCGCGCGTTATGGATACGCGTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGATTACCGGTTCTAACGGCAAAAGCACGGT  >  NC_000913/97174‑97442
                                                                                                                                      |                                                                                                                                      
gtgACGCCGCGCGTTATGGATACGCGTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGc                                                                                                                                   <  1:384320/140‑1 (MQ=255)
     gccgcGCGTTATGGATACGCGTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTgg                                                                                                                              <  1:122169/140‑1 (MQ=255)
     gccgcGCGTTATGGATACGCGTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTgg                                                                                                                              <  1:887312/140‑1 (MQ=255)
       cgcgcgTTATGGATACGCGTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGgcg                                                                                                                            <  2:801802/140‑1 (MQ=255)
         cgcgTTATGGATACGCGTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGTATTGCACTGGCGca                                                                                                                          >  1:274895/1‑140 (MQ=255)
         cgcgTTATGGATACGCGTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGca                                                                                                                          >  2:606752/1‑140 (MQ=255)
          gcgTTATGGATACGCGTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGcat                                                                                                                         <  2:886242/140‑1 (MQ=255)
          gcgTTATGGATACGCGTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGcat                                                                                                                         <  1:59457/140‑1 (MQ=255)
             ttATGGATACGCGTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGcatcca                                                                                                                      <  2:647659/140‑1 (MQ=255)
                      cgcgTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAgc                                                                                                             >  2:535678/1‑140 (MQ=255)
                        cgTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCCTCCATCCTTAAgcgc                                                                                                           >  2:798369/1‑140 (MQ=255)
                        cgTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAgcgc                                                                                                           >  1:996641/1‑140 (MQ=255)
                        cgTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAgcgc                                                                                                           >  1:946496/1‑140 (MQ=255)
                          tATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTg                                                                                                         >  2:361650/1‑140 (MQ=255)
                                    cctggcctggATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGc                                                                                               >  2:372999/1‑140 (MQ=255)
                                    cctggcctggATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGc                                                                                               >  2:466129/1‑140 (MQ=255)
                                    cctggcctggATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGA‑‑‑ATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCgg                                                                                            >  2:511956/1‑140 (MQ=255)
                                         cctggATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGaa                                                                                          >  1:922326/1‑140 (MQ=255)
                                                           aGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGata                                                                        >  2:582386/1‑140 (MQ=255)
                                                           aGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGata                                                                        >  1:407301/1‑140 (MQ=255)
                                                                tAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGTTGATGCCGGAATCGAAATCGTTGGCGATATCGAg                                                                   <  2:616264/140‑1 (MQ=255)
                                                                tAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAg                                                                   <  1:893995/140‑1 (MQ=255)
                                                                tAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGAAGGCGGCAGATCTGATTGTCGCCAGTCCCCGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAg                                                                   <  2:985304/140‑1 (MQ=255)
                                                                 aGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGc                                                                  >  1:19801/1‑140 (MQ=255)
                                                                     cGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGtt                                                              >  1:664529/1‑140 (MQ=255)
                                                                     cGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGtt                                                              <  1:606752/140‑1 (MQ=255)
                                                                      gCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTc                                                             <  2:857734/140‑1 (MQ=255)
                                                                        cacaCGGCCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTg                                                           >  2:287163/1‑140 (MQ=255)
                                                                               gCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGaa                                                    >  1:722402/1‑140 (MQ=255)
                                                                                  gTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGaagca                                                 >  1:901251/1‑140 (MQ=255)
                                                                                  gTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGaagca                                                 >  2:960394/1‑140 (MQ=255)
                                                                                  gTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGaagca                                                 <  1:567380/140‑1 (MQ=255)
                                                                                  gTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGaagca                                                 >  2:843105/1‑140 (MQ=255)
                                                                                             gaATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGAt                                      >  1:131403/1‑140 (MQ=255)
                                                                                             gaATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGAt                                      >  2:206890/1‑140 (MQ=255)
                                                                                             gaATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGAt                                      >  2:1025412/1‑140 (MQ=255)
                                                                                               aTGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATtg                                    >  2:852062/1‑140 (MQ=255)
                                                                                               aTGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATtg                                    <  1:866630/140‑1 (MQ=255)
                                                                                                 ggCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATtgtg                                  >  1:535430/1‑140 (MQ=255)
                                                                                                   cTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGc                                <  2:15376/140‑1 (MQ=255)
                                                                                                    tGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCg                               <  1:949087/140‑1 (MQ=255)
                                                                                                        ggcggcAGATCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGATTa                           >  1:242169/1‑140 (MQ=255)
                                                                                                                aTCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGATTACCGGTTCt                   >  2:1026018/1‑140 (MQ=255)
                                                                                                                aTCTGATTGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGATTACCGGTTCt                   >  2:283068/1‑140 (MQ=255)
                                                                                                                       tGTCGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGATTACCGGTTCTAACGGCa            >  1:885981/1‑140 (MQ=255)
                                                                                                                          cGCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGATTACCGGTTCTAACGGCaaaa         >  2:299718/1‑140 (MQ=255)
                                                                                                                           gCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGATTACCGGTTCTAACGGCAAAAg        >  2:55506/1‑140 (MQ=255)
                                                                                                                           gCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGATTACCGGTTCTAACGGCAAAAg        >  2:1013719/1‑140 (MQ=255)
                                                                                                                           gCCAGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGATTACCGGTTCTAACGGCAAAAg        <  2:664529/140‑1 (MQ=255)
                                                                                                                              aGTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGATTACCGGTTCTAACGGCAAAAGCAc     <  1:843105/140‑1 (MQ=255)
                                                                                                                               gTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGATTACCGGTTCTAACGGCAAAAGCACg    >  2:5509/1‑140 (MQ=255)
                                                                                                                               gTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGATTACCGGTTCTAACGGCAAAAGCACg    <  1:792717/140‑1 (MQ=255)
                                                                                                                               gTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGATTACCGGTTCTAACGGCAAAAGCACg    <  2:484628/140‑1 (MQ=255)
                                                                                                                               gTCCCGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGATTACCGGTTCTAACGGCAAAAGCACg    <  1:716116/140‑1 (MQ=255)
                                                                                                                                 cccGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGATTACCGGTTCTAACGGCAAAAGCACGGt  <  2:131403/140‑1 (MQ=255)
                                                                                                                                 cccGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGATTACCGGTTCTAACGGCAAAAGCACGGt  <  2:946496/140‑1 (MQ=255)
                                                                                                                                 cccGGCATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGCCGCGAAGCACAAGCACCGATTGTGGCGATTACCGGTTCTAACGGCAAAAGCACGGt  <  1:361650/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
GTGACGCCGCGCGTTATGGATACGCGTATGACACCGCCTGGCCTGGATAAATTACCCGAAGCCGTAGAACGCCACACGGGCAGTCTGAATGATGAATGGCTGATGGCGGCAGATCTGATTGTCGCCAGTCCCGGTATTGCACTGGCGCATCCATCCTTAAGCGCTGCCGCTGATGCCGGAATCGAAATCGTTGGCGATATCGAGCTGTTCTGTCGCGAAGCACAAGCACCGATTGTGGCGATTACCGGTTCTAACGGCAAAAGCACGGT  >  NC_000913/97174‑97442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑