Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,486,541	A→G	T1161A (ACG→GCG)	hrpA →	ATP‑dependent RNA helicase HrpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,486,541	0	A	G	100.0%	224.4 / NA	60	T1161A (ACG→GCG)	hrpA	ATP‑dependent RNA helicase HrpA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/31);  total (29/31)

GCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGG  >  NC_000913/1486404‑1486675                                                                                                                                          |                                                                                                                                       gCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCg                                                                                                                                      >  2:602787/1‑140 (MQ=255)  cGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCgg                                                                                                                                     <  2:590609/140‑1 (MQ=255)  cGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCgg                                                                                                                                     <  2:30188/140‑1 (MQ=255)     tgtgGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAg                                                                                                                                  >  2:507528/1‑140 (MQ=255)     tgtgGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAg                                                                                                                                  <  2:148377/140‑1 (MQ=255)     tgtgGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAg                                                                                                                                  >  1:513370/1‑140 (MQ=255)       tgGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAgtg                                                                                                                                >  2:959883/1‑140 (MQ=255)              aTTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAata                                                                                                                         >  1:266608/1‑140 (MQ=255)                    cGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATcaaca                                                                                                                   <  2:426539/140‑1 (MQ=255)                       cGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAAc                                                                                                                <  2:68079/140‑1 (MQ=255)                             tggtggCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGa                                                                                                          <  2:277474/140‑1 (MQ=255)                             tggtggCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGa                                                                                                          <  2:523046/140‑1 (MQ=255)                                 ggCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAgg                                                                                                      <  2:266608/140‑1 (MQ=255)                                 ggCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAgg                                                                                                      <  1:954050/140‑1 (MQ=255)                                  gCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAggg                                                                                                     <  2:741430/140‑1 (MQ=255)                                  gCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAggg                                                                                                     >  2:258923/1‑140 (MQ=255)                                     cGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCgg                                                                                                  <  1:988584/140‑1 (MQ=255)                                      ggTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCggg                                                                                                 <  1:507528/140‑1 (MQ=255)                                      ggTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCggg                                                                                                 <  1:345466/140‑1 (MQ=255)                                      ggTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCggg                                                                                                 <  2:161150/140‑1 (MQ=255)                                       gTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGCGGTGGCTATTGCGAAGCAGGCCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGt                                                                                                >  2:132579/1‑140 (MQ=255)                                               ggAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGa                                                                                        >  1:18078/1‑140 (MQ=255)                                                 aagaagGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGAcc                                                                                      >  1:173526/1‑140 (MQ=255)                                                 aagaagGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGAcc                                                                                      >  2:594514/1‑140 (MQ=255)                                                 aagaagGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGAcc                                                                                      >  1:191017/1‑140 (MQ=255)                                                  agaagGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCa                                                                                     <  1:599225/140‑1 (MQ=255)                                                  agaagGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCa                                                                                     >  2:851629/1‑140 (MQ=255)                                                  agaagGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCa                                                                                     >  1:175891/1‑140 (MQ=255)                                                   gaagGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCAt                                                                                    <  2:755168/140‑1 (MQ=255)                                                      ggCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGgc                                                                                 >  2:754576/1‑140 (MQ=255)                                                        cTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGgcgc                                                                               <  1:812856/140‑1 (MQ=255)                                                         tttGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCt                                                                              >  2:22115/1‑140 (MQ=255)                                                           tgctgcGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGggagga                                                                                             >  1:510606/1‑123 (MQ=255)                                                           tgctgcGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGa                                                                                             <  2:510606/123‑1 (MQ=255)                                                            gctgcgctgcATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTggg                                                                           <  2:191232/140‑1 (MQ=255)                                                             ctgcgctgcATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCTCTgggg                                                                          >  2:495393/1‑140 (MQ=255)                                                                  ctgcATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTc                                                                     <  1:267718/140‑1 (MQ=255)                                                                     cATGAAAAAGTGCGTGCCGAACTGAAGGACACGGTGGTGGATATTGGGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGa                                                                  >  2:832435/1‑140 (MQ=255)                                                                                 cgtgcCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCa                                                      >  1:227581/1‑140 (MQ=255)                                                                                       gAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGTCCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATggg                                                <  1:754576/140‑1 (MQ=255)                                                                                       gAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATggg                                                <  2:409612/140‑1 (MQ=255)                                                                                       gAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATggg                                                <  1:959883/140‑1 (MQ=255)                                                                                         aCTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCg                                              <  2:325562/140‑1 (MQ=255)                                                                                         aCTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCg                                              <  1:22115/140‑1 (MQ=255)                                                                                          cTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCgg                                             <  1:851629/140‑1 (MQ=255)                                                                                           tGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCggg                                            <  2:582992/140‑1 (MQ=255)                                                                                                    cGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTatat                                   <  2:521452/140‑1 (MQ=255)                                                                                                     ggtggtGGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATc                                  >  2:725736/1‑140 (MQ=255)                                                                                                      gtggtgGATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATcg                                 <  2:175891/140‑1 (MQ=255)                                                                                                            gATATTGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGttt                           >  1:78501/1‑140 (MQ=255)                                                                                                                 tGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCa                      >  1:848684/1‑140 (MQ=255)                                                                                                                 tGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCa                      >  1:662917/1‑140 (MQ=255)                                                                                                                 tGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCa                      >  1:412435/1‑140 (MQ=255)                                                                                                                 tGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCa                      >  1:382586/1‑140 (MQ=255)                                                                                                                 tGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCa                      <  2:513370/140‑1 (MQ=255)                                                                                                                 tGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCa                      >  2:824525/1‑140 (MQ=255)                                                                                                                 tGCGAAGCAGGTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCa                      >  2:996220/1‑140 (MQ=255)                                                                                                                           gTCGAGCAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACgg            >  1:358903/1‑140 (MQ=255)                                                                                                                                 cAAATCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCaa      >  2:832227/1‑140 (MQ=255)                                                                                                                                     tCCTTGCGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACgg  <  2:18078/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       GCGGTGTGGATAAATTGATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAAGTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTTACGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGG  >  NC_000913/1486404‑1486675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑