Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,620,380	A→G	V253A (GTA→GCA)	eamA ←	cysteine/O‑acetylserine exporter EamA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,620,380	0	A	G	100.0%	322.9 / NA	86	V253A (GTA→GCA)	eamA	cysteine/O‑acetylserine exporter EamA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/49);  total (37/49)

CCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTACGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAG  >  NC_000913/1620245‑1620515                                                                                                                                        |                                                                                                                                        cccACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCggg                                                                                                                                     <  2:758084/140‑1 (MQ=255) cccACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCggg                                                                                                                                     >  2:100623/1‑140 (MQ=255)    aCCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCAc                                                                                                                                  <  1:677713/140‑1 (MQ=255)      cTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCa                                                                                                                                >  2:127124/1‑140 (MQ=255)         tACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTa                                                                                                                             >  2:928906/1‑140 (MQ=255)         tACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTa                                                                                                                             >  1:144922/1‑140 (MQ=255)                ttACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATaa                                                                                                                      <  1:995946/140‑1 (MQ=255)                ttACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATaa                                                                                                                      <  1:401868/140‑1 (MQ=255)                ttACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATaa                                                                                                                      >  1:197847/1‑140 (MQ=255)                       aCCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCa                                                                                                               <  2:460779/140‑1 (MQ=255)                         cGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAAc                                                                                                             >  1:184892/1‑140 (MQ=255)                         cGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAAc                                                                                                             >  2:835205/1‑140 (MQ=255)                              gCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGcc                                                                                                        <  1:428117/140‑1 (MQ=255)                              gCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGcc                                                                                                        <  2:288592/140‑1 (MQ=255)                              gCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGcc                                                                                                        <  1:91501/140‑1 (MQ=255)                              gCCAAATACATTGATATACAGCCCGGTCATAACGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGcc                                                                                                        <  2:94854/140‑1 (MQ=255)                                cAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAg                                                                                                      >  1:777700/1‑140 (MQ=255)                                      cATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCa                                                                                                <  1:381163/140‑1 (MQ=255)                                       aTTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCAt                                                                                               <  2:944466/140‑1 (MQ=255)                                       aTTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCAt                                                                                               <  1:380575/140‑1 (MQ=255)                                         tGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCATCCCGCCAGGTTTCATAg                                                                                             <  1:565223/140‑1 (MQ=255)                                         tGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAg                                                                                             <  1:110445/140‑1 (MQ=255)                                         tGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAg                                                                                             <  2:685719/140‑1 (MQ=255)                                              taCAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTcc                                                                                        >  2:1025362/1‑140 (MQ=255)                                              taCAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTcc                                                                                        >  2:640240/1‑140 (MQ=255)                                                 aGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAg                                                                                     >  1:190871/1‑140 (MQ=255)                                                   cccGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTa                                                                                   >  1:393069/1‑140 (MQ=255)                                                   cccGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTa                                                                                   >  1:366516/1‑140 (MQ=255)                                                   cccGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTa                                                                                   >  2:878061/1‑140 (MQ=255)                                                   cccGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTa                                                                                   >  1:338576/1‑140 (MQ=255)                                                   cccGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTa                                                                                   >  2:829093/1‑140 (MQ=255)                                                   cccGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTACCAGTa                                                                                   >  2:752268/1‑140 (MQ=255)                                                   cccGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCACCAGGTTTCATAGCGTCCCAGTa                                                                                   >  1:753746/1‑140 (MQ=255)                                                     cGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAAc                                                                                 >  2:330719/1‑140 (MQ=255)                                                     cGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAAc                                                                                 >  1:154748/1‑140 (MQ=255)                                                      ggTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTAATAGCGTCCCAGAAAcc                                                                                >  2:358395/1‑139 (MQ=255)                                                       gTCATAATGTGCACCGCACCTAAAAATGGCAGACCCGTTAAGCGTTCATCCACCAATCGTGCCGCACTGGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGt                                                                               <  2:619436/140‑1 (MQ=255)                                                       gTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGt                                                                               >  1:1001799/1‑140 (MQ=255)                                                         cATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTcc                                                                             <  1:697826/140‑1 (MQ=255)                                                          aTAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTccc                                                                            <  1:870557/140‑1 (MQ=255)                                                             aTGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCa                                                                         <  2:141602/140‑1 (MQ=255)                                                              tGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAg                                                                        <  2:763440/140‑1 (MQ=255)                                                              tGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAg                                                                        <  2:777700/140‑1 (MQ=255)                                                                  caccgcaccTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATcc                                                                    >  2:194732/1‑140 (MQ=255)                                                                  caccgcaccTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATcc                                                                    >  1:345898/1‑140 (MQ=255)                                                                      gcaccTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATa                                                                >  2:994913/1‑140 (MQ=255)                                                                      gcaccTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATa                                                                >  1:91360/1‑140 (MQ=255)                                                                       caccTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATaa                                                               >  2:921535/1‑140 (MQ=255)                                                                       caccTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATaa                                                               >  2:857146/1‑140 (MQ=255)                                                                                    cAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCg                                                  <  2:144922/140‑1 (MQ=255)                                                                                         ccGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCcaca                                             >  1:94546/1‑140 (MQ=255)                                                                                            ttAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAAc                                          >  1:419642/1‑140 (MQ=255)                                                                                            ttAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAAc                                          >  1:659859/1‑140 (MQ=255)                                                                                             tAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACg                                         <  1:563596/140‑1 (MQ=255)                                                                                               aGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGcc                                       <  1:60585/140‑1 (MQ=255)                                                                                               aGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGcc                                       <  1:336366/140‑1 (MQ=255)                                                                                               aGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGcc                                       <  1:194732/140‑1 (MQ=255)                                                                                                 cGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAg                                     <  2:123845/140‑1 (MQ=255)                                                                                                  gTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGa                                    <  2:232920/140‑1 (MQ=255)                                                                                                   ttCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGAt                                   >  2:802093/1‑140 (MQ=255)                                                                                                   ttCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGAt                                   <  2:197847/140‑1 (MQ=255)                                                                                                   ttCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGAt                                   >  1:389810/1‑140 (MQ=255)                                                                                                   ttCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGAt                                   <  1:835205/140‑1 (MQ=255)                                                                                                        ccAACAATAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATc                              <  2:189919/140‑1 (MQ=255)                                                                                                             aaTAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCATCCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAgaga                         <  1:333407/140‑1 (MQ=255)                                                                                                              aTAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGAc                        <  2:900793/140‑1 (MQ=255)                                                                                                              aTAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGAc                        <  1:100623/140‑1 (MQ=255)                                                                                                               tAGTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACa                       <  2:154748/140‑1 (MQ=255)                                                                                                                 gTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAg                     <  1:994913/140‑1 (MQ=255)                                                                                                                 gTGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAg                     >  2:637606/1‑140 (MQ=255)                                                                                                                  tGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGa                    >  2:544098/1‑140 (MQ=255)                                                                                                                  tGCCGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGAT‑CCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGAt                   >  1:143334/1‑140 (MQ=255)                                                                                                                    ccGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGAtg                  >  1:59953/1‑140 (MQ=255)                                                                                                                    ccGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGAtg                  <  1:636528/140‑1 (MQ=255)                                                                                                                    ccGCACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGAtg                  <  2:91360/140‑1 (MQ=255)                                                                                                                       cACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGAtggtg               <  1:640240/140‑1 (MQ=255)                                                                                                                       cACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGAtggtg               <  1:1025362/140‑1 (MQ=255)                                                                                                                       cACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGAtggtg               <  1:921535/140‑1 (MQ=255)                                                                                                                       cACTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGAtggtg               <  1:330868/140‑1 (MQ=255)                                                                                                                         tttGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATggtggt             <  2:338576/139‑1 (MQ=255)                                                                                                                         cTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCTCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATggtggt             >  2:958363/1‑140 (MQ=255)                                                                                                                         cTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATggtggt             <  2:190871/140‑1 (MQ=255)                                                                                                                         cTTGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATggtggt             >  1:18381/1‑140 (MQ=255)                                                                                                                          ttGCCAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTc            <  1:639933/140‑1 (MQ=255)                                                                                                                              cAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCatat        <  1:802093/140‑1 (MQ=255)                                                                                                                              cAGTCCTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCatat        <  2:393069/140‑1 (MQ=255)                                                                                                                              cAGTCCTACTGCGGGAACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCatat        <  2:381271/140‑1 (MQ=255)                                                                                                                                  ccTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGAt    <  1:378299/140‑1 (MQ=255)                                                                                                                                  ccTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGAt    <  2:184892/140‑1 (MQ=255)                                                                                                                                  ccTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGAt    <  1:127124/140‑1 (MQ=255)                                                                                                                                  ccTACTGCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGACGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGAt    <  2:720793/140‑1 (MQ=255)                                                                                                                                   ctactgCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAANNNNNGATACATCAGAGACAAGATGGTGGTCATATCGATa   >  2:745364/1‑140 (MQ=255)                                                                                                                                   ctactgCGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATa   >  2:538803/1‑140 (MQ=255)                                                                                                                                    tactgcGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAg  <  2:419642/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCCTACTACGGGCACCAGTAACGATAACGGTGCAACCCGCCAGGTTTCATAGCGTCCCAGTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAG  >  NC_000913/1620245‑1620515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑