Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,612	T→C	T211A (ACA→GCA)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,612	0	T	C	100.0%	177.5 / NA	48	T211A (ACA→GCA)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/25);  total (23/25)

CAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCC  >  NC_000913/1742476‑1742745                                                                                                                                         |                                                                                                                                      cAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGcc                                                                                                                                    >  2:370409/1‑140 (MQ=255)    gTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTgg                                                                                                                                 >  2:317935/1‑140 (MQ=255)     tGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGt                                                                                                                                <  2:403319/140‑1 (MQ=255)       cTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTg                                                                                                                              >  1:967125/1‑140 (MQ=255)                                 tGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCa                                                                                                    >  2:841060/1‑140 (MQ=255)                                 tGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCa                                                                                                    >  1:658130/1‑140 (MQ=255)                                   gCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGc                                                                                                  >  1:168089/1‑140 (MQ=255)                                      ggTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGcaca                                                                                               >  1:510688/1‑140 (MQ=255)                                        tCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACg                                                                                             <  2:127536/140‑1 (MQ=255)                                        tCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACg                                                                                             <  2:137875/140‑1 (MQ=255)                                          gtgtTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGtt                                                                                           <  1:854004/140‑1 (MQ=255)                                           tgtTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTc                                                                                          >  2:571552/1‑140 (MQ=255)                                             ttCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTa                                                                                        <  2:394991/140‑1 (MQ=255)                                             ttCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTa                                                                                        <  2:162147/140‑1 (MQ=255)                                               ctcACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGTTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTAcc                                                                                      <  2:673070/140‑1 (MQ=255)                                               ctcACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTAcc                                                                                      <  1:910034/140‑1 (MQ=255)                                               ctcACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTAcc                                                                                      <  1:712736/140‑1 (MQ=255)                                               ctcACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTAcc                                                                                      <  2:423863/140‑1 (MQ=255)                                               ctcACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTAcc                                                                                      <  1:293263/140‑1 (MQ=255)                                               ctcACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTCGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTAcc                                                                                      <  1:721491/140‑1 (MQ=255)                                                  aCGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCAAACGTTCTACCGTa                                                                                   >  1:841202/1‑140 (MQ=255)                                                       gtgtTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTAc                                                                              >  1:41098/1‑140 (MQ=255)                                                               aaCGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCt                                                                      <  2:951806/140‑1 (MQ=255)                                                                aCGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTg                                                                     <  1:471117/140‑1 (MQ=255)                                                                  ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTgcg                                                                   >  2:703906/1‑138 (MQ=255)                                                                  ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAg                                                                   >  2:421245/1‑140 (MQ=255)                                                                  ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAg                                                                   >  1:335687/1‑140 (MQ=255)                                                                  ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAg                                                                   >  1:397855/1‑140 (MQ=255)                                                                  ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAg                                                                   >  2:374356/1‑140 (MQ=255)                                                                  ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAg                                                                   >  1:860298/1‑140 (MQ=255)                                                                  ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAg                                                                   >  2:135953/1‑140 (MQ=255)                                                                      ttCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTtg                                                               <  1:65812/140‑1 (MQ=255)                                                                           gTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGAt                                                          <  2:7914/140‑1 (MQ=255)                                                                            tGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATc                                                         <  2:658130/140‑1 (MQ=255)                                                                            tGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCAGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATc                                                         <  1:39701/140‑1 (MQ=255)                                                                                            aTTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGAcg                                         >  2:410308/1‑140 (MQ=255)                                                                                                   cGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTgcgc                                  <  2:510688/140‑1 (MQ=255)                                                                                                            tcCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGttttt                         >  1:76909/1‑140 (MQ=255)                                                                                                             ccGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGtttttt                        <  1:871524/140‑1 (MQ=255)                                                                                                                   gCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTccc                  <  2:771684/140‑1 (MQ=255)                                                                                                                    cGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCa                 >  2:610663/1‑140 (MQ=255)                                                                                                                    cGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCa                 >  1:472335/1‑140 (MQ=255)                                                                                                                      aTTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAag               >  1:430409/1‑140 (MQ=255)                                                                                                                          cAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTc           <  1:527246/140‑1 (MQ=255)                                                                                                                             aTCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTg        <  1:421245/140‑1 (MQ=255)                                                                                                                                 ggCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGCGCGtt    >  2:1032711/1‑140 (MQ=255)                                                                                                                                   cTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTcc  <  2:625681/140‑1 (MQ=255)                                                                                                                                   cTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAACACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTcc  <  1:414125/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      CAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCC  >  NC_000913/1742476‑1742745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑