Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,793,133	C→T	L142L (TTG→TTA)	btuD ←	vitamin B12 ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,793,133	0	C	T	100.0%	150.7 / NA	46	L142L (TTG→TTA)	btuD	vitamin B12 ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/18);  total (28/18)

ATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGCAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGC  >  NC_000913/1792996‑1793265                                                                                                                                          |                                                                                                                                     atcaCAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTaa                                                                                                                                    >  1:446956/1‑140 (MQ=255)       aTCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAAcaccac                                                                                                                              <  1:888895/140‑1 (MQ=255)            cAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCgcag                                                                                                                         <  2:513033/140‑1 (MQ=255)                    gCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGt                                                                                                                 >  1:801828/1‑140 (MQ=255)                       gACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCGAGACGTAcg                                                                                                              <  1:635529/140‑1 (MQ=255)                         cacaGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGCCGTAcgcg                                                                                                            >  1:39206/1‑140 (MQ=255)                         cacaGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTAcgcg                                                                                                            <  1:101271/140‑1 (MQ=255)                           caGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGtt                                                                                                          >  2:694400/1‑140 (MQ=255)                             gcgcgcTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGc                                                                                                        >  2:19451/1‑140 (MQ=255)                             gcgcgcTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGc                                                                                                        >  2:337310/1‑140 (MQ=255)                              cgcgcTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGcc                                                                                                       >  2:830517/1‑140 (MQ=255)                              cgcgcTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGcc                                                                                                       >  1:962865/1‑140 (MQ=255)                                 gcTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCAtt                                                                                                    <  2:530203/140‑1 (MQ=255)                                  cTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTc                                                                                                   >  1:240437/1‑140 (MQ=255)                                   tCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTAcg                                                                                                              <  2:195821/128‑1 (MQ=255)                                   tCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTAcg                                                                                                              >  1:195821/1‑128 (MQ=255)                                   tCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCa                                                                                                  <  2:873553/140‑1 (MQ=255)                                                      tcTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCCGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTgg                                                                               <  2:467995/139‑1 (MQ=255)                                                              gcgcAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGt                                                                       <  1:436589/140‑1 (MQ=255)                                                                gcAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTcc                                                                     <  2:1006153/140‑1 (MQ=255)                                                                    cATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCGCCGCCGGAAAGTTGATTGGTGCTACGTCCGAGt                                                                 >  2:887281/1‑140 (MQ=255)                                                                              gTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCaa                                                       >  2:113784/1‑140 (MQ=255)                                                                               ttCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAag                                                      >  1:115014/1‑140 (MQ=255)                                                                               ttCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAag                                                      >  1:933874/1‑140 (MQ=255)                                                                               ttCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAag                                                      >  2:786100/1‑140 (MQ=255)                                                                                  aTCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGc                                                   >  2:885854/1‑140 (MQ=255)                                                                                    cGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCa                                                 >  1:102647/1‑140 (MQ=255)                                                                                    cGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGCTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCa                                                 >  1:860449/1‑140 (MQ=255)                                                                                      gCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAgc                                               <  1:127227/140‑1 (MQ=255)                                                                                         catcaAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGcc                                            >  2:1023104/1‑140 (MQ=255)                                                                                         catcaAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGcc                                            >  2:37083/1‑140 (MQ=255)                                                                                             aagaagCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTg                                        <  1:887281/140‑1 (MQ=255)                                                                                             aagaagCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTg                                        <  1:571705/140‑1 (MQ=255)                                                                                                aagCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGa                                     <  1:830517/140‑1 (MQ=255)                                                                                                 agcagcAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGAc                                    >  2:338118/1‑140 (MQ=255)                                                                                                  gcagcaATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGAca                                   <  2:240437/140‑1 (MQ=255)                                                                                                    agcaATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGAcatc                                 >  2:467210/1‑140 (MQ=255)                                                                                                    agcaATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGAcatc                                 >  1:216128/1‑140 (MQ=255)                                                                                                    agcaATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGAcatc                                 >  1:341646/1‑140 (MQ=255)                                                                                                     gcaATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGAcatca                                <  2:39206/140‑1 (MQ=255)                                                                                                       aaTTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCAtt                              <  2:993808/140‑1 (MQ=255)                                                                                                                gCGGGATTGGCTTGTGGTGTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACATCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTc                     >  1:787088/1‑140 (MQ=255)                                                                                                                             gtggtgTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATCCAGTAGTTCGGTACGCGTTTTa        <  1:744706/140‑1 (MQ=255)                                                                                                                              tggtgTGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTAt       >  1:440170/1‑140 (MQ=255)                                                                                                                                gtgtGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCg     >  1:534756/1‑140 (MQ=255)                                                                                                                                gtgtGATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCg     >  1:560470/1‑140 (MQ=255)                                                                                                                                   tgATTTGTAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGc  >  2:236177/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     ATCACAATCGCCAGTCCTTGCTGACACAGCGCGCTCAGAATTTTGTCTAACGCACTTTGTTGCGCAACATCAAGACTGTTCATCGGCTCATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGCAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGC  >  NC_000913/1792996‑1793265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑