Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	213,575	T→C	G415G (GGT→GGC)	tilS →	tRNA(Ile)‑lysidine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	213,575	0	T	C	100.0%	149.1 / NA	41	G415G (GGT→GGC)	tilS	tRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/21);  total (20/21)

TAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACC  >  NC_000913/213443‑213704                                                                                                                                     |                                                                                                                                  tAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTg                                                                                                                            <  2:520399/140‑1 (MQ=255)  aaCGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGa                                                                                                                           >  1:509318/1‑140 (MQ=255)  aaCGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGa                                                                                                                           >  1:28692/1‑140 (MQ=255)           cGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGaa                                                                                                                  <  1:297252/140‑1 (MQ=255)                 cTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGt                                                                                                            <  1:1032372/140‑1 (MQ=255)                    aaGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAg                                                                                                         >  1:225938/1‑140 (MQ=255)                          aTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGt                                                                                                   >  1:583753/1‑140 (MQ=255)                          aTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGt                                                                                                   <  1:730972/140‑1 (MQ=255)                            cTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCt                                                                                                 <  2:799837/140‑1 (MQ=255)                              ggCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTgg                                                                                               >  2:557244/1‑140 (MQ=255)                                  agagCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGa                                                                                           <  1:20296/140‑1 (MQ=255)                                   gagCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGaa                                                                                          <  1:23548/140‑1 (MQ=255)                                       tGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACTCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACg                                                                                      <  2:760674/140‑1 (MQ=255)                                        gggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGc                                                                                     <  1:195329/140‑1 (MQ=255)                                        gggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGc                                                                                     <  2:483139/140‑1 (MQ=255)                                         ggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCt                                                                                    <  2:661798/140‑1 (MQ=255)                                         ggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCCTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCt                                                                                    <  2:832715/140‑1 (MQ=255)                                          gCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCtt                                                                                   <  1:917208/140‑1 (MQ=255)                                              gtcgccgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGtt                                                                               <  1:149402/138‑1 (MQ=255)                                                   cgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTg                                                                          >  1:10248/1‑140 (MQ=255)                                                      ggCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGaaa                                                                       <  2:275075/140‑1 (MQ=255)                                                        cTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGc                                                                     <  2:272165/140‑1 (MQ=255)                                                                 accacGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGa                                                            >  2:18053/1‑140 (MQ=255)                                                                   cacGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACg                                                          <  2:15351/140‑1 (MQ=255)                                                                     cGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGGGTAAGTTTTTGATTGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCa                                                        >  2:784938/1‑140 (MQ=255)                                                                     cGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCa                                                        >  1:574964/1‑140 (MQ=255)                                                                          ctgctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCaaa                                                   >  2:903596/1‑140 (MQ=255)                                                                          ctgctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGGCGGATAAGACGCAGCaaa                                                   >  2:877936/1‑140 (MQ=255)                                                                           tgctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAAc                                                  >  2:232533/1‑140 (MQ=255)                                                                                ttttATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGc                                             >  1:658451/1‑140 (MQ=255)                                                                                          aaaCGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGGTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGaa                                   >  1:558206/1‑140 (MQ=255)                                                                                                 gATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAAt                            <  2:10248/140‑1 (MQ=255)                                                                                                        gcggcAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGAtt                     <  2:28692/140‑1 (MQ=255)                                                                                                        gcggcAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGAtt                     >  1:598197/1‑140 (MQ=255)                                                                                                          ggcAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCg                   <  2:509318/140‑1 (MQ=255)                                                                                                          ggcAGGGGTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCg                   <  2:718642/140‑1 (MQ=255)                                                                                                            ctggggTATTTGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCt                 >  2:726816/3‑140 (MQ=255)                                                                                                                    tttGTGACGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccacca         >  1:592116/1‑140 (MQ=255)                                                                                                                          aCGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTAc   >  1:984804/1‑140 (MQ=255)                                                                                                                          aCGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTAc   >  1:141757/1‑140 (MQ=255)                                                                                                                           cGCAAGAAGGCGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTAcc  >  1:862239/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                  TAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACC  >  NC_000913/213443‑213704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑