Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,906,870	G→A	A64V (GCC→GTC)	rlmA ←	23S rRNA m(1)G745 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,906,870	0	G	A	100.0%	186.7 / NA	55	A64V (GCC→GTC)	rlmA	23S rRNA m(1)G745 methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/30);  total (25/30)

GGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGAC  >  NC_000913/1906738‑1906999                                                                                                                                     |                                                                                                                                  ggCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTaa                                                                                                                            >  2:696745/1‑140 (MQ=255)     aCGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAAt                                                                                                                        >  1:179484/1‑140 (MQ=255)     aCGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAAt                                                                                                                        >  2:1036653/1‑140 (MQ=255)        cATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATACCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATgcg                                                                                                                     <  2:599397/140‑1 (MQ=255)           cGGCAAATGCGTGTGTGTAATACCCTTCACCACGGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCgcgg                                                                                                                  <  1:516176/140‑1 (MQ=255)            ggCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCgcggc                                                                                                                 >  2:627448/1‑140 (MQ=255)              cAAATGCGTGTGTGTAATACCCTCCCCCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGt                                                                                                               <  2:31408/140‑1 (MQ=255)                aaTGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGc                                                                                                             >  2:584036/1‑140 (MQ=255)                     gtgtgtttAATCCCCTTCCCCACAGCCAATACCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGca                                                                                                        <  2:1037803/140‑1 (MQ=255)                        tgtgtAATACCCTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGcatca                                                                                                     >  2:587156/1‑140 (MQ=255)                                ccccTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCcgcg                                                                                             <  1:705521/139‑1 (MQ=255)                                ccccTTCACCACAGCCAATACCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCcgcg                                                                                             <  1:280313/139‑1 (MQ=255)                                 cccTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCgcgc                                                                                            >  1:909538/1‑140 (MQ=255)                                  ccTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCt                                                                                           <  2:204493/140‑1 (MQ=255)                                  ccTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCt                                                                                           <  1:460305/140‑1 (MQ=255)                                    ttCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGt                                                                                         >  2:997829/1‑140 (MQ=255)                                    ttCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGt                                                                                         >  1:193893/1‑140 (MQ=255)                                      caccacAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCg                                                                                       >  1:149442/1‑140 (MQ=255)                                      caccacAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCg                                                                                       >  2:594360/1‑140 (MQ=255)                                        ccacAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGcc                                                                                     <  2:463606/140‑1 (MQ=255)                                          acaGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTCGCATCATTTCCGCGCTGTCGCCCg                                                                                   <  1:346203/140‑1 (MQ=255)                                           caGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCgg                                                                                  >  1:945195/1‑140 (MQ=255)                                            aGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGa                                                                                 >  1:872748/1‑140 (MQ=255)                                            aGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTACGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGa                                                                                 <  1:777320/140‑1 (MQ=255)                                            aGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCATTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGa                                                                                 >  1:745614/1‑140 (MQ=255)                                                   atCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACgag                                                                          <  2:303072/140‑1 (MQ=255)                                                        gCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGt                                                                     >  2:225376/1‑140 (MQ=255)                                                        gCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGt                                                                     >  2:350302/1‑140 (MQ=255)                                                              ccgTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGc                                                               >  1:9392/1‑140 (MQ=255)                                                                   gCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAAc                                                          <  2:179484/140‑1 (MQ=255)                                                                       tATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGgc                                                      <  1:466833/140‑1 (MQ=255)                                                                       tATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGgc                                                      <  1:1031753/140‑1 (MQ=255)                                                                        atcatcAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGgca                                                     >  1:616929/1‑140 (MQ=255)                                                                         tcatcaAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGgcag                                                    <  1:997829/140‑1 (MQ=255)                                                                             caAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGa                                                <  1:796119/140‑1 (MQ=255)                                                                              aaGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGAt                                               >  1:826308/1‑140 (MQ=255)                                                                               aGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGAtt                                              >  1:156107/1‑140 (MQ=255)                                                                                 ccGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGa                                            <  1:243951/140‑1 (MQ=255)                                                                                       ccTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATAcc                                      <  1:1003015/140‑1 (MQ=255)                                                                                                ggCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCg                             <  1:587156/140‑1 (MQ=255)                                                                                                ggCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCg                             <  1:627448/140‑1 (MQ=255)                                                                                                ggCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCg                             >  2:805088/1‑140 (MQ=255)                                                                                                ggCGACAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCg                             <  2:890903/140‑1 (MQ=255)                                                                                                     cAATTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCata                        >  1:436348/1‑140 (MQ=255)                                                                                                       aTTGCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATc                      >  1:597013/1‑140 (MQ=255)                                                                                                          gCATCACGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCaaa                   <  2:616929/140‑1 (MQ=255)                                                                                                                cGCAGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATg             >  1:602871/1‑140 (MQ=255)                                                                                                                   aGCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCCTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTCACAAACCCTTCTTTCGCCATATCAAACTGATGTCg          >  2:277649/1‑140 (MQ=255)                                                                                                                    gCGGCTGATAATGTCCGACTTCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGc         <  2:888353/140‑1 (MQ=255)                                                                                                                    gCGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGc         <  2:297905/140‑1 (MQ=255)                                                                                                                     cGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCt        <  1:1036653/140‑1 (MQ=255)                                                                                                                     cGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCt        <  2:945195/140‑1 (MQ=255)                                                                                                                     cGGCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCt        <  1:594360/140‑1 (MQ=255)                                                                                                                      ggCTGATAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTg       <  1:961030/140‑1 (MQ=255)                                                                                                                           aTAATGTCCGACATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGAc  <  1:350302/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                  GGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGAC  >  NC_000913/1906738‑1906999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑