Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,040,269	A→G	P264P (CCA→CCG)	msrP →	periplasmic protein‑L‑methionine sulfoxide reductase catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,040,269	0	A	G	100.0%	134.4 / NA	36	P264P (CCA→CCG)	msrP	periplasmic protein‑L‑methionine sulfoxide reductase catalytic subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/18);  total (18/18)

ATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGA  >  NC_000913/2040135‑2040391                                                                                                                                       |                                                                                                                           atgaCCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGaccac                                                                                                                       >  1:61741/1‑140 (MQ=255) atgaCCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGaccac                                                                                                                       >  1:979798/1‑140 (MQ=255)  ggaCGGTGGGTGTTTAGGGCAGGGCTTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGaccacc                                                                                                                      <  2:730702/139‑1 (MQ=255)  ggaCCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGaccacc                                                                                                                      <  1:388576/139‑1 (MQ=255)     ccGTAGGGGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTgg                                                                                                                   >  1:716810/1‑140 (MQ=255)      cGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGa                                                                                                                  >  1:117605/1‑140 (MQ=255)         aGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATc                                                                                                               >  2:268998/1‑140 (MQ=255)         aGGTGTTTATGGCAAGGCGTAACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATc                                                                                                               >  2:655359/1‑140 (MQ=255)                tATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAgc                                                                                                        >  1:669948/1‑140 (MQ=255)                     cAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTg                                                                                                   <  2:465647/140‑1 (MQ=255)                        ggCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACg                                                                                                >  2:1013461/1‑140 (MQ=255)                             tACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATAc                                                                                           <  2:381706/140‑1 (MQ=255)                                        aaTGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGc                                                                                <  2:140265/140‑1 (MQ=255)                                        aaTGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGc                                                                                <  2:875389/140‑1 (MQ=255)                                        aaTGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGc                                                                                <  2:302360/140‑1 (MQ=255)                                        aaTGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGc                                                                                <  1:268998/140‑1 (MQ=255)                                         aTGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGcc                                                                               <  2:300974/140‑1 (MQ=255)                                          tGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCa                                                                              <  1:252570/140‑1 (MQ=255)                                               cgcCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGtt                                                                         <  1:910334/140‑1 (MQ=255)                                                  cGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAAt                                                                      <  1:49573/140‑1 (MQ=255)                                                                 tgCCGTTGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCAc                                                       >  2:211810/1‑140 (MQ=255)                                                                  gCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTGTGTTGATCAcc                                                      <  1:735154/140‑1 (MQ=255)                                                                  gCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCAcc                                                      <  2:48265/140‑1 (MQ=255)                                                                      tGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCcgcg                                                  <  2:61741/140‑1 (MQ=255)                                                                        gAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCt                                                >  2:931921/1‑140 (MQ=255)                                                                        gAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCt                                                >  2:642713/1‑140 (MQ=255)                                                                                  tttAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGc                                      >  2:70563/1‑140 (MQ=255)                                                                                   ttAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCt                                     >  1:225313/1‑140 (MQ=255)                                                                                         ggATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGaa                               <  1:1013461/140‑1 (MQ=255)                                                                                                    aTAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTgg                    >  1:407990/1‑140 (MQ=255)                                                                                                          aGTATTAAGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAgg              <  2:440009/140‑1 (MQ=255)                                                                                                                 aGCTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATc       >  2:942586/1‑140 (MQ=255)                                                                                                                     gACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCg   <  1:655359/140‑1 (MQ=255)                                                                                                                      aCCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGa  >  2:746362/1‑140 (MQ=255)                                                                                                                      aCCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGa  >  2:246568/1‑140 (MQ=255)                                                                                                                      aCCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGa  >  1:816898/1‑140 (MQ=255)                                                                                                                      aCCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGa  >  1:820600/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                           ATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGA  >  NC_000913/2040135‑2040391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑