Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,246,532	T→C	T80A (ACG→GCG)	cirA ←	ferric dihyroxybenzoylserine outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,246,532	0	T	C	100.0%	243.8 / NA	65	T80A (ACG→GCG)	cirA	ferric dihyroxybenzoylserine outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/38);  total (27/38)

AGTTCAGATCGAAATCATTGTGGCGGAAGACGGCATTGCGGGAGTTCACGCGTTTACCGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGTCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGAC  >  NC_000913/2246400‑2246667                                                                                                                                     |                                                                                                                                        aGTTCAGATCGAAATCATTGTGGCGGAAGACGGCATTGCGGGAGTTCACGCGTTTACCGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGt                                                                                                                                  <  1:34418/140‑1 (MQ=255)   ttCAGATCGAAATCATTGTGGCGGAAGACGGCATTGCGGGAGTTCACGCGTTTACCGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTAc                                                                                                                                >  2:383967/1‑140 (MQ=255)      aGATCGAAATCATTGTGGCGGAAGACGGCATTGCGGGAGTTCACGCGTTTACCGTCGACGAGAATCAGGGGATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGcc                                                                                                                             >  1:929043/1‑140 (MQ=255)        aTCGAAATCATTGTGGCGGAAGACGGCATTGCGGGAGTTCACGCGTTTACCGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAg                                                                                                                           >  1:167131/1‑140 (MQ=255)        aTCGAAATCATTGTGGCGGAAGACGGCATTGCGGGAGTTCACGCGTTTACCGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAg                                                                                                                           >  2:422681/1‑140 (MQ=255)         tCGAAATCATTGTGGCGGAAGACGGCATTGCGGGAGTTCACGCGTTTACCGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAgg                                                                                                                          <  1:933225/140‑1 (MQ=255)          cGAAATCATTGTGGCGGAAGACGGCATTGCGGGAGTTCACGCGTTTACCGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGc                                                                                                                         <  1:578274/140‑1 (MQ=255)           gAAATCATTGTGGCGGAAGACGGCATTGCGGGAGTTCACGCGTTTACCGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCa                                                                                                                        <  1:393468/140‑1 (MQ=255)           gAAATCATTGTGGCGGAAGACGGCATTGCGGGAGTTCACGCGTTTACCGTCGACGAGAATCACGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCa                                                                                                                        <  2:908345/140‑1 (MQ=255)              aTCATTGTGGCGGAAGACGGCATTGCGGGAGTTCACGCGTTTACCGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCActt                                                                                                                     <  2:87939/140‑1 (MQ=255)                  ttGTGGCGGAAGACGGCATTGCGGGAGTTCACGCGTTTACCGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCttt                                                                                                                 <  1:60329/140‑1 (MQ=255)                       gCGGAAGACGGCATTGCGGGAGTTCACGCGTTTACCGTCGACGAGGATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGa                                                                                                            >  2:79446/1‑140 (MQ=255)                         ggAAGACGGCATTGCGGGAGTTCACGCGTTTACCGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACa                                                                                                          <  2:1005587/140‑1 (MQ=255)                          gAAGACGGCATTGCGGGAGTTCACGCGTTTACCGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACAt                                                                                                         <  1:579764/140‑1 (MQ=255)                          gAAGACGACATTGCGGGAGTTCACGCGTTTACCGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACAt                                                                                                         <  2:188137/140‑1 (MQ=255)                               cGGCATTGCGGGAGTTCACGCGTTTACCGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTc                                                                                                    <  2:1015309/140‑1 (MQ=255)                                  cATTGCGGGAGTTCACGCGTTTACCGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGa                                                                                                 <  1:962969/140‑1 (MQ=255)                                  cATTGCGGGAGTTCACGCGTTTACCGTCGACGAGAATCAGGGAATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAgc                                                                                                 >  2:367180/1‑139 (MQ=255)                                            gTTCACGCGTTTACCGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCg                                                                                       <  1:1035029/140‑1 (MQ=255)                                                    gTTTACCGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGc                                                                               <  2:729763/140‑1 (MQ=255)                                                      ttACCGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTg                                                                             <  1:268125/140‑1 (MQ=255)                                                       tACCGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGc                                                                            >  2:570112/1‑140 (MQ=255)                                                          cGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTCCGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAgg                                                                         <  2:915896/140‑1 (MQ=255)                                                          cGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAgg                                                                         <  1:887411/140‑1 (MQ=255)                                                           gTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGt                                                                        <  1:922434/140‑1 (MQ=255)                                                            tCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTc                                                                       <  2:671934/140‑1 (MQ=255)                                                            tCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTc                                                                       >  2:129951/1‑140 (MQ=255)                                                               acgaGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTc                                                                    <  1:860388/140‑1 (MQ=255)                                                                  agaATCAGGGTATGGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTg                                                                 <  1:877882/140‑1 (MQ=255)                                                                  agaATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTg                                                                 <  2:562117/140‑1 (MQ=255)                                                                       cAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCTCTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTaa                                                            <  1:590141/140‑1 (MQ=255)                                                                        aGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAAt                                                           <  1:590028/140‑1 (MQ=255)                                                                        aGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAAt                                                           <  2:1013582/140‑1 (MQ=255)                                                                            tataGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCGGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACg                                                       <  2:644323/140‑1 (MQ=255)                                                                            tataGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACg                                                       <  1:203598/140‑1 (MQ=255)                                                                             ataGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGc                                                      <  1:425665/140‑1 (MQ=255)                                                                             ataGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGc                                                      <  2:242713/140‑1 (MQ=255)                                                                              taGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCt                                                     >  1:824925/1‑140 (MQ=255)                                                                               aGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTg                                                    >  1:776262/1‑140 (MQ=255)                                                                                    ctgTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACt                                               >  2:690746/1‑140 (MQ=255)                                                                                      gTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTgg                                             >  2:437093/1‑140 (MQ=255)                                                                                       tCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGc                                            <  2:630177/140‑1 (MQ=255)                                                                                       tCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGc                                            <  1:422681/140‑1 (MQ=255)                                                                                        ccAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCa                                           >  2:474211/1‑140 (MQ=255)                                                                                           gACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGt                                        <  1:79446/140‑1 (MQ=255)                                                                                             ccACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGc                                      <  1:217261/140‑1 (MQ=255)                                                                                                 gAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCt                                  >  1:225734/1‑140 (MQ=255)                                                                                                      cTAACGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGCGCATCTTTTAg                             >  1:542015/1‑140 (MQ=255)                                                                                                        aaCGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGAt                           >  2:97495/1‑140 (MQ=255)                                                                                                         aCGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGAtt                          >  1:771356/1‑140 (MQ=255)                                                                                                          cGCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGAttt                         >  2:1031632/1‑140 (MQ=255)                                                                                                           gCCCTTACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGGGCATCTTTAAGAtttt                        >  2:941188/1‑140 (MQ=255)                                                                                                               ttACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTc                    >  2:520127/1‑140 (MQ=255)                                                                                                               ttACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTc                    >  2:520713/1‑140 (MQ=255)                                                                                                               ttACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTc                    >  1:218396/1‑140 (MQ=255)                                                                                                               ttACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGGGCATCTTTAAGATTTTGTTc                    >  2:79395/1‑140 (MQ=255)                                                                                                                tACGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTcc                   <  1:1012851/140‑1 (MQ=255)                                                                                                                  cGGTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCAc                 <  1:81999/140‑1 (MQ=255)                                                                                                                    gTTATCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACgg               <  1:45780/140‑1 (MQ=255)                                                                                                                       aTCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAg            >  2:474485/1‑140 (MQ=255)                                                                                                                       aTCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAg            >  1:922496/1‑140 (MQ=255)                                                                                                                        tCCCCTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGa           <  2:776262/140‑1 (MQ=255)                                                                                                                          cccTTCGTTCGCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATg         <  2:1035389/140‑1 (MQ=255)                                                                                                                             ttcgttcgCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAg      >  1:528681/1‑140 (MQ=255)                                                                                                                             ttcgttcgCCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAg      >  1:446073/1‑140 (MQ=255)                                                                                                                               cgttcgcCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTg    >  1:978478/1‑140 (MQ=255)                                                                                                                                 ttcgcCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGAc  >  2:432066/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        AGTTCAGATCGAAATCATTGTGGCGGAAGACGGCATTGCGGGAGTTCACGCGTTTACCGTCGACGAGAATCAGGGTATAGCTGCTGTCCAGACCACGAATACTAACGCCCTTACGGTTATCCCCTTCGTTCGTCAGTTGTACGCCAGGCACTTCTTTGAGGACATCCTTCAGATTCTGTACCGGTTTTCGCTGCAGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGAC  >  NC_000913/2246400‑2246667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑