Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,262,214	T→C	D51G (GAC→GGC)	fruK ←	1‑phosphofructokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,262,214	0	T	C	100.0%	113.1 / NA	31	D51G (GAC→GGC)	fruK	1‑phosphofructokinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/11);  total (20/11)

CAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTG  >  NC_000913/2262078‑2262342                                                                                                                                         |                                                                                                                                 cAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCtt                                                                                                                               >  1:85628/1‑140 (MQ=255)    cTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTa                                                                                                                            <  1:632257/140‑1 (MQ=255)               ttCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGcc                                                                                                                 <  2:319593/140‑1 (MQ=255)               ttCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGcc                                                                                                                 <  2:328317/140‑1 (MQ=255)               ttCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGcc                                                                                                                 <  2:758889/140‑1 (MQ=255)                tCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCa                                                                                                                <  1:613263/140‑1 (MQ=255)                     tGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTg                                                                                                           <  2:895791/140‑1 (MQ=255)                     tGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTg                                                                                                           <  2:808703/140‑1 (MQ=255)                     tGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTg                                                                                                           <  2:207199/140‑1 (MQ=255)                     tGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTg                                                                                                           <  2:516768/140‑1 (MQ=255)                     tGCGCCCCTGTACAACCTGGAAACGGCTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTg                                                                                                           >  2:20248/1‑140 (MQ=255)                                    ccTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTGATGCCTTTACccgcc                                                                                            >  1:578607/1‑140 (MQ=255)                                    ccTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTGATGCCTTTACccgcc                                                                                            >  2:160131/1‑140 (MQ=255)                                    ccTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTGATGCCTTTACccgcc                                                                                            >  2:732564/1‑140 (MQ=255)                                    ccTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTGATGCCTTTACccgcc                                                                                            >  1:255868/1‑140 (MQ=255)                                      tGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTGATGCCTTTACCcgccgc                                                                                          >  1:58349/1‑140 (MQ=255)                                       ggAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCa                                                                                         >  2:608513/1‑140 (MQ=255)                                        gAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCAt                                                                                        >  2:779103/1‑140 (MQ=255)                                           aCGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGTTTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATCCCCAGGCCTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCATGCa                                                                                     <  1:139618/140‑1 (MQ=255)                                              gTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGGGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCATGCAGAc                                                                                  >  2:909637/1‑140 (MQ=255)                                                 gccAATGCCCAGCTCGCCGAACAGTTGCTGAAAGCCTTCCTGTTTCTCTTAACCCAGAAAGCCGCCAGGGGTCACATCAATCCCCGGCCCTTTTAATTTTTGGGCCCCTTTGATGCTTTTCCCCGCCGCATGCAGACCgg                                                                               <  2:85628/138‑1 (MQ=255)                                                      tGCCCAGCTCGCTGAACAGTTGCTAAAAACCATCCTGTTTGTCTTTCCCCAGGAAGCCGCCAACGGTGACACCAATCCCCAGGCTTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGtt                                                                          <  2:58349/140‑1 (MQ=255)                                                                gCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGt                                                                >  1:498352/1‑140 (MQ=255)                                                                 cTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGtt                                                               >  2:964023/1‑140 (MQ=255)                                                                  tGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTc                                                              >  1:735021/1‑140 (MQ=255)                                                                                aaaCCTTCCTGTTTGTCTTTCCCCAGGAACCCGCCAACGGTGACATCAATCCCCGGGCCTTTTAATTCTTGGCCCACGTTGATGCTTTTCCCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGtcc                                                <  2:313460/140‑3 (MQ=255)                                                                                   ccATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAAt                                             >  1:602141/1‑140 (MQ=255)                                                                                           aTTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCACCGCGTTCAATTTCCGGGc                                     >  2:468342/1‑140 (MQ=255)                                                                                             tGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAg                                   >  2:898285/1‑140 (MQ=255)                                                                                                       cAGGAAGCCGCCAACGGTGACATCAATCCCCAGGCCTTTTAATTCTTTGCCCACGTCGATGCCTTTCCCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACcaacaa                         <  2:94998/140‑1 (MQ=255)                                                                                                         ggAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAgg                       >  1:770270/1‑140 (MQ=255)                                                                                                         ggAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAgg                       >  2:114166/1‑140 (MQ=255)                                                                                                          gAAGCCGCCAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGt                      >  2:108280/1‑140 (MQ=255)                                                                                                            aGCCCCCAACGGTAACATCAATCCCCAGACTTTTTAAAATTTTGGCCACGTTATTCCATTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCa                    <  1:909637/140‑1 (MQ=255)                                                                                                             gccgccAACGGTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCAt                   >  1:257801/1‑140 (MQ=255)                                                                                                                 ccAGCGGTGACATCAATCCCCGGCCCTTTTAATCCTTGGGCCCCGTTGACGCCTTTCCCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGc               <  2:664186/140‑1 (MQ=255)                                                                                                                     cGGTGACATCAATCCCCAGGCCTTTTAATACTTTGCCCCCGTTGATGCCTTTCCCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGa           <  1:653112/140‑1 (MQ=255)                                                                                                                       gTGACATCAATTCCCAGGCCTTTTAATACTTTGGCCACGTTGATGCCTTTCCCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGAtt         <  1:20248/140‑1 (MQ=255)                                                                                                                        tGACATCAATCCCCAGGCTTTTTAATACTTGGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTa        <  2:498352/140‑1 (MQ=255)                                                                                                                        tGACATCAATCCCCAGGCCTTTTAATATTTTGGCCCCGTTGATGCTTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTa        <  2:602141/140‑1 (MQ=255)                                                                                                                           cttaaaTCCCCAGGCCTTTAAATATTTGGGCCAGGTTGATCCCTTTCCCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAgg     <  1:797943/136‑1 (MQ=255)                                                                                                                              aaaTTCCCAGGCTTTTTAATTTTTTGGCCAAGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTg  <  1:608513/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 CAGCTTAACGTTAATTCGGGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGCTGAAAACCATCCTGATTGTCTTTACCCAGGAAGCCGCCAACGGTGACATCAATTCCCAGGTCTTTTAATACTTTGGCCACGTTGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTG  >  NC_000913/2262078‑2262342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑