| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,297,107 | T→C | I180V (ATT→GTT) | ccmA ← | heme trafficking system ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,297,107 | 0 | T | C | 97.9% | 169.3 / ‑3.1 | 47 | coding (538/624 nt) | ccmA | heme trafficking system ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (18/28); minor base . (0/1); total (18/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
CGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGACGCGG > NC_000913/2296970‑2297240 | cgcTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAccc > 1:872014/1‑140 (MQ=255) ggCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCt < 1:351280/140‑1 (MQ=255) ggCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCt < 2:575054/140‑1 (MQ=255) ggCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCt < 1:840263/140‑1 (MQ=255) ggCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCt < 2:251031/140‑1 (MQ=255) gCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTg < 1:752379/140‑1 (MQ=255) aaCGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTcc < 2:270692/140‑1 (MQ=255) cTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTAt > 1:928055/1‑140 (MQ=255) cacGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCCGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTAtgct < 2:773823/140‑1 (MQ=255) cacGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTAtgct < 2:182411/140‑1 (MQ=255) gCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTgcg > 2:1022241/1‑140 (MQ=255) cAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTgcgc < 1:380108/140‑1 (MQ=255) cAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAA‑CCCCCCCTGCTCCGTATGCTGCGcc < 1:972713/140‑1 (MQ=255) aGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGcc > 1:569772/1‑140 (MQ=255) aGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCCGCGcc > 2:41468/1‑140 (MQ=255) aCGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTg > 1:368972/1‑140 (MQ=255) aaaaTGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTc > 1:634751/1‑140 (MQ=255) aaaaTGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTc > 2:410099/1‑140 (MQ=255) aaaaTGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTc > 1:526568/1‑140 (MQ=255) aaaaTGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTc < 2:502054/140‑1 (MQ=255) aGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGa > 2:530730/1‑140 (MQ=255) gAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGac < 2:103399/140‑1 (MQ=255) catGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGtt < 2:755118/140‑1 (MQ=255) tGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTaa > 2:968265/1‑140 (MQ=255) ggCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGt > 1:616643/1‑140 (MQ=255) cccTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCa > 2:372977/1‑140 (MQ=255) cccTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCa > 1:97043/1‑140 (MQ=255) ccTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCaa > 2:106045/1‑140 (MQ=255) ccTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCaa < 1:1022241/140‑1 (MQ=255) tCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATc > 2:48743/1‑140 (MQ=255) cGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATcg < 2:926284/140‑1 (MQ=255) tCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTaaaa < 2:216010/140‑1 (MQ=255) tCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTaaaa < 2:569772/140‑1 (MQ=255) aGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAgg < 2:634751/140‑1 (MQ=255) tGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCt < 1:530730/140‑1 (MQ=255) tGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCtcgtcg > 2:284265/1‑140 (MQ=255) tGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCtcgtcg < 2:981044/140‑1 (MQ=255) gcggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGa < 1:200840/140‑1 (MQ=255) gcggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGa < 2:368972/140‑1 (MQ=255) cggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAg > 2:497248/1‑140 (MQ=255) cggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAg < 2:249032/140‑1 (MQ=255) aaTTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATcc > 2:444465/1‑140 (MQ=255) tACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAAGCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAAc < 2:97043/140‑1 (MQ=255) gCTGGTGGGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGa < 1:48743/140‑1 (MQ=255) tggtggGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGTGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGAcg < 2:941039/140‑1 (MQ=255) gtggGTAGTCAGAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGAcgcg < 1:41468/140‑1 (MQ=255) gAATCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGAcg < 1:667142/140‑1 (MQ=255) tCACAACCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGACgcgg < 2:526568/140‑1 (MQ=255) | CGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGACGCGG > NC_000913/2296970‑2297240 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |