Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,363,421	T→C	G71G (GGA→GGG)	yfaY ←	IPR008135 CinA family protein YfaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,363,421	0	T	C	100.0%	66.8 / NA	19	G71G (GGA→GGG)	yfaY	IPR008135 CinA family protein YfaY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/4);  total (15/4)

TTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGC  >  NC_000913/2363284‑2363555                                                                                                                                          |                                                                                                                                       ttACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGccc                                                                                                                                      >  1:70415/1‑140 (MQ=255)       ttGCTCGGGGCCATTCCCCGCCCCCGTTCGGGAAAATAGCTTCCCTTCTTTTGGAGCCGGGTTTCATGCAGCACCAGGCCTTCCCTTTTTGCTGCCGCGGCGGCGAGGGCGTTAAAATCATCGCTGGTCGGCCCCAGccc                                                                                                                                <  2:106353/140‑1 (MQ=255)          cTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGCCCCAGCccgcc                                                                                                                             >  1:206223/1‑140 (MQ=255)             ggTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGCCCCAGCCCGCCGtt                                                                                                                          >  1:308051/1‑140 (MQ=255)               tGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGCCCCAGCCCGCCGTTaa                                                                                                                        >  1:397395/1‑140 (MQ=255)                           tCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGCCCCAGCCCGCCGTTAACGATCAGCACAt                                                                                                            >  1:646197/1‑140 (MQ=255)                             cACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGCCCCAGCCCGCCGTTAACGATCAGCACATCg                                                                                                          >  2:13090/1‑140 (MQ=255)                               cGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACTTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGCCCCAGCCCGCCGTTAACGATCAGCACATCGGc                                                                                                        >  2:591752/1‑140 (MQ=255)                                            aGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGACCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGt                                                                                           >  2:13453/1‑140 (MQ=255)                                                                   gCTTCATGCAGCACCAGGCTTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGCCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGtc                                                                    >  2:974961/1‑140 (MQ=255)                                                                     ttCATGCGGCCCCAGCCCTTCCCTTTTGGCGGGCGCGGCGGGGAGTGCGCTTAAATCATCGGTGGTCGGCCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGtcat                                                                  <  2:308051/140‑1 (MQ=255)                                                                       cATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGCCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGtcatca                                                                >  2:204932/1‑140 (MQ=255)                                                                               aCCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGCCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATc                                                        >  1:729318/1‑140 (MQ=255)                                                                                ccAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGCCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATcc                                                       >  1:693933/1‑140 (MQ=255)                                                                                        tCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGCCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGt                                               >  2:307573/1‑140 (MQ=255)                                                                                        tCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGCCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGt                                               >  1:220994/1‑140 (MQ=255)                                                                                        tCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCCGCCCCAGCCCGCCGTTTACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGTCTAAGTCATCAAGGTTATCCCCCACCga                                               >  2:248286/1‑139 (MQ=255)                                                                                           ctttttGCTGCCGCAGCGGCGGGTGCGTTAAAATCTCCGCGGGTCGCCCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTAtt                                            <  1:13090/138‑1 (MQ=255)                                                                                               ttGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGCCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTgcgg                                        >  1:338246/1‑140 (MQ=255)                                                                                               ttGCGGCCGCGGGGGCGAGTGCTTTAAAATCACCGCGGGTCGGCCCCCCCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTgcgg                                        <  2:38183/140‑1 (MQ=255)                                                                                                tgctgcCGCGGCGGCGAGTGCGTTAAAATCTTCGCGGGTCGCCCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTgcggc                                       <  1:591752/140‑1 (MQ=255)                                                                                                      cGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGCCCCAGCCCGCCGGTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATa                                 >  1:108082/1‑140 (MQ=255)                                                                                                           gggcgGGTGCGTTTAAATCCCCGCGGGTCGGCCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGc                            <  1:687627/139‑1 (MQ=255)                                                                                                           cggcgAGTGCGCTTAAATCATCGCTGGTCGACCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGc                            <  1:13453/140‑1 (MQ=255)                                                                                                                                   gggtcggccCCGCCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCg    <  2:206223/139‑1 (MQ=255)                                                                                                                                     gtcggccCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGgc  >  2:912583/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       TTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGC  >  NC_000913/2363284‑2363555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑