Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,475,069	T→C	V29A (GTT→GCT)	yfdR →	CPS‑53 (KpLE1) prophage; 5'‑deoxynucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,475,069	0	T	C	100.0%	241.4 / NA	65	V29A (GTT→GCT)	yfdR	CPS‑53 (KpLE1) prophage; 5'‑deoxynucleotidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/36);  total (29/36)

TTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAG  >  NC_000913/2474933‑2475203                                                                                                                                         |                                                                                                                                       ttCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTc                                                                                                                                     <  2:876661/140‑1 (MQ=255) ttCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTc                                                                                                                                     <  1:475755/140‑1 (MQ=255)  tCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTcc                                                                                                                                    <  2:304462/140‑1 (MQ=255)  tCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTcc                                                                                                                                    <  2:923689/140‑1 (MQ=255)   ccTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTccc                                                                                                                                   >  2:880110/1‑140 (MQ=255)   ccTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTccc                                                                                                                                   <  1:35701/140‑1 (MQ=255)                    gcgcGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGc                                                                                                                  >  1:208335/1‑140 (MQ=255)                     cgcgGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCt                                                                                                                 >  1:61567/1‑140 (MQ=255)                        ggTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGTCGCTTTg                                                                                                              <  2:567193/140‑1 (MQ=255)                            cAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCgg                                                                                                          <  2:762755/140‑1 (MQ=255)                             aGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGt                                                                                                         >  1:361642/1‑140 (MQ=255)                              gcgcATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTc                                                                                                        <  1:220901/140‑1 (MQ=255)                               cgcATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCa                                                                                                       <  1:315379/140‑1 (MQ=255)                                 cATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATc                                                                                                     >  1:250478/1‑140 (MQ=255)                                    ataCGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCttt                                                                                                  >  2:620226/1‑140 (MQ=255)                                    ataCGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCttt                                                                                                  >  1:529902/1‑140 (MQ=255)                                    ataCGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCttt                                                                                                  <  2:625973/140‑1 (MQ=255)                                    ataCGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCttt                                                                                                  >  2:807901/1‑140 (MQ=255)                                    ataCGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCttt                                                                                                  >  1:811593/1‑140 (MQ=255)                                      aCGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTtct                                                                                                <  1:339533/140‑1 (MQ=255)                                      aCGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTtct                                                                                                <  2:915574/140‑1 (MQ=255)                                      aCGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTtct                                                                                                <  1:850141/140‑1 (MQ=255)                                      aCGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTtct                                                                                                <  2:405873/140‑1 (MQ=255)                                         tGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCAc                                                                                             <  2:923820/140‑1 (MQ=255)                                         tGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCAc                                                                                             <  2:527335/140‑1 (MQ=255)                                         tGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCAc                                                                                             <  1:607009/140‑1 (MQ=255)                                         tGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCAc                                                                                             <  1:273922/140‑1 (MQ=255)                                           gagaACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACtt                                                                                           >  1:244589/1‑140 (MQ=255)                                            agaACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTc                                                                                          >  1:676659/1‑140 (MQ=255)                                            agaACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTc                                                                                          >  2:202809/1‑140 (MQ=255)                                              aaCAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTa                                                                                        >  1:764158/1‑140 (MQ=255)                                                cAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACa                                                                                      <  2:250478/140‑1 (MQ=255)                                                  aaaTGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAgt                                                                                    <  2:90879/140‑1 (MQ=255)                                                   aaTGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAgtg                                                                                   >  2:765859/1‑140 (MQ=255)                                                   aaTGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAgtg                                                                                   <  1:880110/140‑1 (MQ=255)                                                               taAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATg                                                                       <  2:1021779/140‑1 (MQ=255)                                                               taAAACGTTTTCCGGGAAGCATTATTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATg                                                                       <  1:255875/140‑1 (MQ=255)                                                                       tttCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCtt                                                               >  2:872172/1‑140 (MQ=255)                                                                       tttCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCtt                                                               >  2:946460/1‑140 (MQ=255)                                                                       tttCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCtt                                                               <  2:232695/140‑1 (MQ=255)                                                                        ttCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCttt                                                              >  2:868618/1‑140 (MQ=255)                                                                        ttCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCttt                                                              >  2:612450/1‑140 (MQ=255)                                                                             ggAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGCTTCCATTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGcagc                                                         <  1:37934/140‑1 (MQ=255)                                                                                    tttttATTATGACTAGATAAATAATGTCGCATTCGTGATTACCGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGTCGGTCATCTTTCTCACTTCTGCAGTGTCGCCCAACCTGCGGTGCTTTGCAGCCAGGTgg                                                  <  2:361642/140‑1 (MQ=255)                                                                                                    ataaataaAGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGTCGCAGGAATTTGc                                  >  2:196853/1‑140 (MQ=255)                                                                                                         taaaGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTg                             <  2:764158/140‑1 (MQ=255)                                                                                                         aaaaGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTTCAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTg                             <  1:303203/139‑1 (MQ=255)                                                                                                           aaGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGaa                           <  1:868618/140‑1 (MQ=255)                                                                                                            aGACGACATCGTGATTACCGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAg                          <  2:61567/140‑1 (MQ=255)                                                                                                            aGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAg                          <  2:340364/140‑1 (MQ=255)                                                                                                            aGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAg                          <  2:676659/140‑1 (MQ=255)                                                                                                            aGACGACATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAg                          <  2:529902/140‑1 (MQ=255)                                                                                                             gacgacATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGc                         >  2:495754/1‑140 (MQ=255)                                                                                                              acgacATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAg                          >  2:682934/1‑138 (MQ=255)                                                                                                              acgacATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAg                          <  1:682934/138‑1 (MQ=255)                                                                                                              acgacATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCa                        >  1:423698/1‑140 (MQ=255)                                                                                                              acgacATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGACATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCa                        >  2:774992/1‑140 (MQ=255)                                                                                                               cgacATCGTGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCAt                       <  2:244589/140‑1 (MQ=255)                                                                                                                       tGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCat               <  1:202809/140‑1 (MQ=255)                                                                                                                       tGATTAACGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCat               >  1:431334/1‑140 (MQ=255)                                                                                                                            aaCGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGc          >  1:996842/1‑140 (MQ=255)                                                                                                                            aaCGATATCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGc          >  1:770315/1‑140 (MQ=255)                                                                                                                                 tatCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAg     <  1:696311/140‑1 (MQ=255)                                                                                                                                  atCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCTCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGa    <  1:946460/140‑1 (MQ=255)                                                                                                                                   tCGCGGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGaa   >  1:112479/1‑140 (MQ=255)                                                                                                                                    cgcgGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAg  >  1:720407/1‑140 (MQ=255)                                                                                                                                    cgcgGCTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAg  >  1:831717/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCTTTCTCACTTCTACAGTGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAG  >  NC_000913/2474933‑2475203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑