Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,674,352	G→A	H113Y (CAC→TAC)	yphB ←	putative aldose 1‑epimerase YphB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,674,352	0	G	A	100.0%	207.2 / NA	60	H113Y (CAC→TAC)	yphB	putative aldose 1‑epimerase YphB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/37);  total (23/37)

GCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCA  >  NC_000913/2674217‑2674488                                                                                                                                        |                                                                                                                                         gcgTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAg                                                                                                                                      >  1:495883/1‑140 (MQ=255)         ggcgACAACGAAAAAAAGGGAGGCCAGCGGGTACAAAAGGGCGGCGTCTCTGCCCCTTGATGGGTGACAGAGGGCGTCCCCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCt                                                                                                                              <  2:1012056/140‑1 (MQ=255)            gACAAGGAAAAATAAGGATCCCAGCGGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCg                                                                                                                           <  2:748220/140‑1 (MQ=255)            gACAACGGAAAAAAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCCCCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCg                                                                                                                           <  2:523716/140‑1 (MQ=255)             aCAACGAAAAATAAGGATGCCAGCCGGTCCCAAATGGCAGCGTCTCGGCCCCTTGATGGGGGACAGAGGGCGTCCCCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGa                                                                                                                          <  2:896639/140‑1 (MQ=255)                       aTAGGGATGCCAGCGGGTACAAAATGGCGGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCGCCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATaca                                                                                                                <  2:487911/140‑1 (MQ=255)                         agggATGCCAGCCGGTACAAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACAcc                                                                                                              <  2:890548/138‑1 (MQ=255)                         agggAGGCCAGCCGGTACCAAATGGCAGCGTCTCGCCCCCTTGATTGGTGACAGAGAGCGTCCCCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACAcc                                                                                                              <  2:243330/138‑1 (MQ=255)                          gggACGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCa                                                                                                             <  2:439093/139‑1 (MQ=255)                            gaggCCAGCCGGTCCCAAAGGGCGGCGTCTCTCCCCCTTGATGGGTGACAGAGGGCGCCCCCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGa                                                                                                           <  2:1026318/137‑1 (MQ=255)                              tGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCCCCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGaca                                                                                                         <  2:82269/140‑1 (MQ=255)                              ggCCAGCGGGTCCAAAATGGCGGCGTCTCTGCCGCTGGTTTGGTGCCGGAAAGCGTCACCTTCTGCAACTCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACGCCGCTGCGATGTTCATACACCAGaca                                                                                                         <  2:223299/139‑1 (MQ=255)                              cgCCAGCAGGTACCAAATGGCGGCGTCTCTGCCCCTTGATTGGTGAAAGAGGGGGTCACGGACGGCCTACCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGaca                                                                                                         <  2:552594/139‑1 (MQ=255)                                ccAGCGGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCCCCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGAcaca                                                                                                       <  1:587846/140‑1 (MQ=255)                                           cAAATGGCGGCGTCTCTGCCCTTTGATTGGTGACAGAGAGCGTCCCCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCg                                                                                            <  2:738094/140‑1 (MQ=255)                                           cAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCCCCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCg                                                                                            <  2:14451/140‑1 (MQ=255)                                           aaaaGGGCAGCGTCTCGCCCCCTTGATTGGTGACAGAGAGCGCCCCCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCg                                                                                            <  2:927202/139‑1 (MQ=255)                                             aaTGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCt                                                                                          >  2:789640/1‑140 (MQ=255)                                              aTGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCtg                                                                                         >  1:702515/1‑140 (MQ=255)                                              aTGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGCCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCtg                                                                                         <  2:829508/140‑1 (MQ=255)                                                  cAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCg                                                                                     >  1:803659/1‑140 (MQ=255)                                                  cAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCg                                                                                     >  1:543843/1‑140 (MQ=255)                                                  cAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCg                                                                                     >  1:160004/1‑140 (MQ=255)                                                     cGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCgaga                                                                                  >  2:192039/1‑140 (MQ=255)                                                       tctcTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGaca                                                                                >  1:982798/1‑140 (MQ=255)                                                           tGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACAtt                                                                            >  2:1012616/1‑140 (MQ=255)                                                                cTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCAc                                                                       >  2:844548/1‑140 (MQ=255)                                                                  tGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTc                                                                     >  2:599077/1‑140 (MQ=255)                                                                  tGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTCCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTc                                                                     >  2:16454/1‑140 (MQ=255)                                                                   gATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCg                                                                    >  2:1002636/1‑140 (MQ=255)                                                                        gTGACAGAGAGCGTCCCCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCccag                                                               <  1:602424/140‑1 (MQ=255)                                                                          gACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGccc                                                                 >  2:236632/1‑136 (MQ=255)                                                                          gACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGccc                                                                 <  1:236632/136‑1 (MQ=255)                                                                              gagagCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCagccagcc                                                         >  2:674486/1‑140 (MQ=255)                                                                               agagCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCg                                                                    >  2:925697/1‑128 (MQ=255)                                                                               agagCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCg                                                                    <  1:925697/128‑1 (MQ=255)                                                                                 ggcctccccGTCAGCGTACCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATc                                                      <  2:160004/133‑1 (MQ=255)                                                                                 agCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATc                                                      <  1:608878/140‑1 (MQ=255)                                                                                  gCGTCCCCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCg                                                     <  2:80974/140‑1 (MQ=255)                                                                                  gCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCg                                                     >  1:590345/1‑140 (MQ=255)                                                                                   cgcccccGTCAGGGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGc                                                    <  2:866409/135‑1 (MQ=255)                                                                                   cGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGc                                                    <  2:543843/140‑1 (MQ=255)                                                                                   cGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGc                                                    >  2:767203/1‑140 (MQ=255)                                                                                           gcAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGa                                            <  1:496547/139‑1 (MQ=255)                                                                                             aGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATa                                          >  2:387985/1‑140 (MQ=255)                                                                                               cGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGt                                        >  1:366208/1‑140 (MQ=255)                                                                                                  aTCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTgcg                                     <  1:675426/140‑1 (MQ=255)                                                                                                    ccgccgTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTCTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCa                                   <  2:760199/140‑1 (MQ=255)                                                                                                      gccgTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAACCATCGCCGTGTAGATAGTGCGCATc                                 <  1:660504/140‑1 (MQ=255)                                                                                                         gTTAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCa                              >  1:302604/1‑140 (MQ=255)                                                                                                           tAAATGAAACGCCTGACTTACCCGATAGTAATAGACCCCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACt                            <  2:803659/140‑1 (MQ=255)                                                                                                           tAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACt                            <  2:590345/140‑1 (MQ=255)                                                                                                           tAAATGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACt                            <  1:674486/140‑1 (MQ=255)                                                                                                            aaaTGAAACGCCTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTc                           >  1:700097/1‑140 (MQ=255)                                                                                                                      ccTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGt                 <  1:844548/140‑1 (MQ=255)                                                                                                                       cTGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGtt                <  1:1012616/140‑1 (MQ=255)                                                                                                                        tGACTTACCCGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTg               <  2:495883/140‑1 (MQ=255)                                                                                                                         gACTTACCCGATAGTAATAGACGCCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCAGCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGc              <  1:619747/140‑1 (MQ=255)                                                                                                                               cccGATAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGa        <  2:366208/140‑1 (MQ=255)                                                                                                                                    tAGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGTCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTc   >  1:973811/1‑140 (MQ=255)                                                                                                                                     aGTAATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCa  <  2:982798/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCA  >  NC_000913/2674217‑2674488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑