Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,680,114	G→A	P878S (CCG→TCG)	yphG ←	DUF5107 domain‑containing protein YphG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,680,114	0	G	A	100.0%	159.5 / NA	47	P878S (CCG→TCG)	yphG	DUF5107 domain‑containing protein YphG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/19);  total (28/19)

GATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAG  >  NC_000913/2679985‑2680246                                                                                                                                  |                                                                                                                                     gATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTg                                                                                                                            >  2:1015588/1‑140 (MQ=255)   tAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTgcg                                                                                                                          <  2:963100/140‑1 (MQ=255)   tAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTgcg                                                                                                                          <  2:383261/140‑1 (MQ=255)      tGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACCAGTGGCATTTGCGCGt                                                                                                                       >  2:317508/1‑140 (MQ=255)           cgcgGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCga                                                                                                                  >  2:787863/1‑140 (MQ=255)                 aTGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGt                                                                                                            <  1:472605/140‑1 (MQ=255)                           tCGCAGGCCTGCTGCGGCTGTCTGACATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCttt                                                                                                  <  2:266034/140‑1 (MQ=255)                            cGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTg                                                                                                 <  1:225723/140‑1 (MQ=255)                              cAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTc                                                                                               >  2:956264/1‑140 (MQ=255)                                  ccTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGcc                                                                                           <  2:693693/140‑1 (MQ=255)                                  ccTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGcc                                                                                           <  1:864029/140‑1 (MQ=255)                                   ctgctgCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCt                                                                                          <  1:1015588/140‑1 (MQ=255)                                    tgctgcGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTg                                                                                         >  2:978393/1‑140 (MQ=255)                                      ctgcGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGAc                                                                                       >  1:660801/1‑140 (MQ=255)                                      ctgcGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGAc                                                                                       >  1:399607/1‑140 (MQ=255)                                           gCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTg                                                                                  >  1:380264/1‑140 (MQ=255)                                            cTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTga                                                                                 >  2:107975/1‑140 (MQ=255)                                             tGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTgag                                                                                <  1:125857/140‑1 (MQ=255)                                             tGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTgag                                                                                <  2:921509/140‑1 (MQ=255)                                                  ggCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGcc                                                                           <  2:66007/140‑1 (MQ=255)                                                         aGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAAtt                                                                    >  2:307636/1‑140 (MQ=255)                                                            tgctgcCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAg                                                                 <  2:63870/140‑1 (MQ=255)                                                            tgctgcCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAg                                                                 <  1:787863/140‑1 (MQ=255)                                                            tgctgcCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTCCCCTTCCCCGCCTCCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAg                                                                 <  2:629817/140‑1 (MQ=255)                                                              ctgcCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCa                                                               >  1:929231/1‑140 (MQ=255)                                                                 ccAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGtt                                                            >  1:206671/1‑140 (MQ=255)                                                                    ggCGCGTAATAACTGGTTGAGGATAATCTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTg                                                         <  1:317508/140‑1 (MQ=255)                                                                      cgcgTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCg                                                       >  2:141268/1‑140 (MQ=255)                                                                                   gTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTcgc                                          >  2:860464/1‑140 (MQ=255)                                                                                   gTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTcgc                                          >  2:577679/1‑140 (MQ=255)                                                                                    ttGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTcgcg                                         >  1:601700/1‑140 (MQ=255)                                                                                        ggATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTc                                     >  1:212316/1‑140 (MQ=255)                                                                                                  actgGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAAttt                           >  1:500099/1‑140 (MQ=255)                                                                                                  actgGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAAttt                           >  1:806518/1‑140 (MQ=255)                                                                                                   ctgGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTc                          >  1:995632/1‑140 (MQ=255)                                                                                                   ctgGTGACCTTCCCTTCCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTc                          >  1:992030/1‑140 (MQ=255)                                                                                                          ccttcccttcCCCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGatta                   <  2:380264/140‑1 (MQ=255)                                                                                                            ttcccttcccCGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGattatt                 >  2:412798/1‑140 (MQ=255)                                                                                                                   ccccGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGa          <  2:790743/140‑1 (MQ=255)                                                                                                                   ccccGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGa          <  1:216484/140‑1 (MQ=255)                                                                                                                   ccccGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGa          <  2:500099/140‑1 (MQ=255)                                                                                                                      cGCCTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGAcgc       >  1:122824/1‑140 (MQ=255)                                                                                                                        ccTTCCCACGAGTGGACTTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGCTTCAGCGCAATTTCCAGATTATTTTCCAGACgcgc     >  2:234501/1‑140 (MQ=255)                                                                                                                        ccTTCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACgcgc     >  1:125538/1‑140 (MQ=255)                                                                                                                           tCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAg  >  2:279746/1‑140 (MQ=255)                                                                                                                           tCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAg  >  1:94492/1‑140 (MQ=255)                                                                                                                           tCCCACGAGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAg  >  1:102718/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                     GATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAG  >  NC_000913/2679985‑2680246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑