Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,745,777	A→G	F48L (TTT→CTT)	rimM ←	ribosome maturation factor RimM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,745,777	0	A	G	100.0%	188.8 / NA	51	F48L (TTT→CTT)	rimM	ribosome maturation factor RimM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/24);  total (27/24)

TTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAACAGGTGCTTGCGCGGTGAGTTG  >  NC_000913/2745656‑2745909                                                                                                                          |                                                                                                                                     ttCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCaaa                                                                                                                    <  1:540865/140‑1 (MQ=255)  tCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCaaaa                                                                                                                   <  1:504676/140‑1 (MQ=255)   cGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCaaaaa                                                                                                                  >  1:516968/1‑140 (MQ=255)           ggTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCg                                                                                                          <  2:913253/140‑1 (MQ=255)            gTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCgg                                                                                                         >  1:275329/1‑140 (MQ=255)               cgccgcATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGt                                                                                                      >  1:276997/1‑140 (MQ=255)                 ccgcATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCt                                                                                                    >  1:502876/1‑140 (MQ=255)                    cATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGTTGATCGTCAAAAATGCTTTCGGCGTCTTCg                                                                                                 <  1:923833/140‑1 (MQ=255)                     aTCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCgg                                                                                                <  1:214080/140‑1 (MQ=255)                     aTCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCgg                                                                                                <  2:499398/140‑1 (MQ=255)                       cACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTg                                                                                              <  1:686029/140‑1 (MQ=255)                               tCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGCAGaaaa                                                                                      <  1:311352/140‑1 (MQ=255)                               tCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGaaaa                                                                                      >  2:897926/1‑140 (MQ=255)                                 aaCGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAaca                                                                                    >  1:58783/1‑140 (MQ=255)                                    gCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTACCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACActc                                                                                 <  1:250211/140‑1 (MQ=255)                                      cTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTg                                                                               >  2:887898/1‑140 (MQ=255)                                        ttCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAg                                                                             <  2:468484/140‑1 (MQ=255)                                        ttCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAg                                                                             >  2:386347/1‑140 (MQ=255)                                        ttCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAg                                                                             <  2:1011506/140‑1 (MQ=255)                                        ttCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAg                                                                             >  1:517917/1‑140 (MQ=255)                                        ttCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAg                                                                             <  1:450244/140‑1 (MQ=255)                                         tCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGc                                                                            <  2:523525/140‑1 (MQ=255)                                           aGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGcca                                                                          <  2:570880/140‑1 (MQ=255)                                               tgatgaTCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGccaccca                                                                      <  2:915719/140‑1 (MQ=255)                                                 atgatCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACg                                                                    <  1:235926/140‑1 (MQ=255)                                                   gatCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGaa                                                                  <  1:452023/140‑1 (MQ=255)                                                       aTGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATAcc                                                              >  2:686110/1‑140 (MQ=255)                                                           ccTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTaa                                                          >  2:697620/1‑140 (MQ=255)                                                           ccTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTaa                                                          >  1:683163/1‑140 (MQ=255)                                                               aTTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACg                                                      >  2:393741/1‑140 (MQ=255)                                                                 tgtgGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGaa                                                    <  2:58783/140‑1 (MQ=255)                                                                  gtggtgCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAAc                                                   >  1:936848/1‑140 (MQ=255)                                                                  gtggtgCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAAc                                                   <  2:258082/140‑1 (MQ=255)                                                                    ggtgCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAAccc                                                 >  2:930623/1‑140 (MQ=255)                                                                    ggtgCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAAccc                                                 >  1:10768/1‑140 (MQ=255)                                                                    ggtgCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAAccc                                                 >  2:213041/1‑140 (MQ=255)                                                                         ttccagctttccagctGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCAtttt                                            >  1:733046/1‑140 (MQ=255)                                                                         ttccagctttccagctGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCAtttt                                            >  1:396124/1‑140 (MQ=255)                                                                           ccagctttccagctGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTc                                          >  2:169812/1‑140 (MQ=255)                                                                                  tccagctGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAAc                                   >  1:362356/1‑140 (MQ=255)                                                                                   ccagctGGACTTGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACg                                  <  2:159629/140‑1 (MQ=255)                                                                                             ttGCTGCCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCaa                        <  1:534942/140‑1 (MQ=255)                                                                                              tgctgcCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAAc                       <  2:51758/140‑1 (MQ=255)                                                                                               gctgcCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAACa                      >  1:250730/1‑140 (MQ=255)                                                                                                ctgcCACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAACAg                     >  1:104097/1‑140 (MQ=255)                                                                                                    cACTGACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAACAGGTGc                 >  1:470778/1‑140 (MQ=255)                                                                                                        gACCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAACAGGTGCTTgt             <  2:516968/140‑2 (MQ=255)                                                                                                         aCCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAACAGGTGCTTgcg            >  2:516703/1‑140 (MQ=255)                                                                                                         aCCCGCCTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAACAGGTGCTTgcg            >  2:662003/1‑140 (MQ=255)                                                                                                               cTTCTGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAACAGGTGCTTGCGCGGTGa      <  1:697620/140‑1 (MQ=255)                                                                                                                   tGGATAAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAACAGGTGCTTGCGCGGTGAgttg  >  1:303833/1‑140 (MQ=255)                                                                                                                          |                                                                                                                                     TTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGATCAACAGGTGCTTGCGCGGTGAGTTG  >  NC_000913/2745656‑2745909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑