Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,763,764	A→G	F464L (TTT→CTT)	abpA ←	CP4‑57 prophage; anti‑bacteriophage protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,763,764	0	A	G	100.0%	135.0 / NA	36	F464L (TTT→CTT)	abpA	CP4‑57 prophage; anti‑bacteriophage protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/23);  total (13/23)

ACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTC  >  NC_000913/2763632‑2763899                                                                                                                                     |                                                                                                                                        aCGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGaaa                                                                                                                                  >  2:857683/1‑140 (MQ=255)   ggCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACg                                                                                                                                >  2:410222/1‑140 (MQ=255)             cGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGa                                                                                                                      <  1:1022784/140‑1 (MQ=255)              gATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAg                                                                                                                     <  1:375825/140‑1 (MQ=255)                tGAGGTCAGCCGCGAATTCTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCa                                                                                                                   <  1:16155/140‑1 (MQ=255)                            cgAATTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTgg                                                                                                       <  1:236855/140‑1 (MQ=255)                              aaTTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCt                                                                                                     >  1:103329/1‑140 (MQ=255)                              aaTTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCt                                                                                                     >  2:317488/1‑140 (MQ=255)                              aaTTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCt                                                                                                     >  1:535673/1‑140 (MQ=255)                               aTTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTg                                                                                                    <  1:875289/140‑1 (MQ=255)                                 ttttCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGcc                                                                                                  <  1:631869/140‑1 (MQ=255)                                      gAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCaa                                                                                             <  2:485689/140‑1 (MQ=255)                                       aaCAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAg                                                                                            <  1:731668/140‑1 (MQ=255)                                          aGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAAt                                                                                         <  2:465719/140‑1 (MQ=255)                                                 cTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCa                                                                                  <  2:226409/140‑1 (MQ=255)                                                  tGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCaa                                                                                 <  1:368010/140‑1 (MQ=255)                                                     ttctttctCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGt                                                                              <  2:566436/140‑1 (MQ=255)                                                          tctcCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTgg                                                                         <  1:857683/140‑1 (MQ=255)                                                               aGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAg                                                                    <  1:723871/140‑1 (MQ=255)                                                                           aaCCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATc                                                        <  2:103329/140‑1 (MQ=255)                                                                           aaCCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATc                                                        <  2:403542/140‑1 (MQ=255)                                                                                    gTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAAt                                               >  1:342049/1‑140 (MQ=255)                                                                                               ggTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAttt                                    >  1:924205/1‑140 (MQ=255)                                                                                                 ttAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAttttt                                  <  2:638804/140‑1 (MQ=255)                                                                                                      agATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTgcg                             <  1:552742/140‑1 (MQ=255)                                                                                                       gATTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTgcgc                            <  2:535673/140‑1 (MQ=255)                                                                                                        aTTAGATTCGTATCTCACGAGCAGAACAAGTGTTAAACGATCGATGTGAGCTTCGAAAGGTTTGCTGCCATCAATAATTTCGTCAATTTCGTTGCGAAGCAGGTATTTATCGTCTGTAATGTCGAGGATTTTTTTgcgcg                           <  2:218325/140‑1 (MQ=255)                                                                                                        aTTAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTgcgcg                           <  2:286926/140‑1 (MQ=255)                                                                                                         ttAGAGTCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTgcgcgc                          >  1:79614/1‑140 (MQ=255)                                                                                                               tCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTgcgcg                           >  1:769065/1‑133 (MQ=255)                                                                                                               tCGTAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTgcgcg                           <  2:769065/133‑1 (MQ=255)                                                                                                                  tAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTGCGCGCGGTATCGAt                 >  1:371458/1‑140 (MQ=255)                                                                                                                  tAGCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTGCGCGCGGTATCGAt                 >  1:474538/1‑140 (MQ=255)                                                                                                                    gCCCACGAACAGAACAAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATTGTCTTTAATGTCGAGGATTTTTTTGCGCGCGGTATCGATGc               <  1:437074/140‑1 (MQ=255)                                                                                                                                aacaAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGt   <  2:371458/140‑1 (MQ=255)                                                                                                                                aacaAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGt   >  1:668162/1‑140 (MQ=255)                                                                                                                                 acaAGCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTc  >  1:1018336/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        ACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTTTCTTTCTCCAGGTCATCCTCGAACCCCGGCGTTTCAGGCTCGGTTAGTAGATTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTC  >  NC_000913/2763632‑2763899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑