Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,844,492	C→T	T87T (ACG→ACA)	hycF ←	formate hydrogenlyase subunit HycF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,844,492	0	C	T	100.0%	156.0 / NA	46	T87T (ACG→ACA)	hycF	formate hydrogenlyase subunit HycF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/18);  total (28/18)

CGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTCAACCGTTAAGGCGTTTGACGGGCAGGCATTGACGCAGGCCGCGC  >  NC_000913/2844356‑2844625                                                                                                                                         |                                                                                                                                      ggTAGTGGATCCCTGTCTGGACGGCGAAAGGCCGATTGCAGACGCGGCAGTTGCACGGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTGCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTc                                                                                                                                    <  2:675657/139‑1 (MQ=255)    aGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCggg                                                                                                                                 <  1:850972/140‑1 (MQ=255)        gATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGa                                                                                                                             <  2:227022/140‑1 (MQ=255)                    gACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGc                                                                                                                 <  1:168173/140‑1 (MQ=255)                    gACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGc                                                                                                                 >  1:259662/1‑140 (MQ=255)                        gCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTcc                                                                                                             >  2:863248/1‑140 (MQ=255)                        gCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTcc                                                                                                             >  1:199288/1‑140 (MQ=255)                        gCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTcc                                                                                                             >  2:684896/1‑140 (MQ=255)                        gCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTcc                                                                                                             >  2:447378/1‑140 (MQ=255)                        gCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTcc                                                                                                             <  2:447141/140‑1 (MQ=255)                        gCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTcc                                                                                                             >  1:94751/1‑140 (MQ=255)                        gCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTcc                                                                                                             >  1:861176/1‑140 (MQ=255)                         cGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCca                                                                                                            >  2:973654/1‑140 (MQ=255)                         cGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCca                                                                                                            >  1:401836/1‑140 (MQ=255)                          gAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCcac                                                                                                           <  2:18517/140‑1 (MQ=255)                              ggACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACagaa                                                                                                       >  1:551233/1‑140 (MQ=255)                                              ggCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGa                                                                                       >  2:384317/1‑140 (MQ=255)                                              ggCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGa                                                                                       >  2:562761/1‑140 (MQ=255)                                               gCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGAt                                                                                      >  1:384754/1‑140 (MQ=255)                                                            gcgAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGc                                                                         <  2:113525/140‑1 (MQ=255)                                                               aggCGGGGCTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTCCTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAg                                                                      <  2:554279/138‑1 (MQ=255)                                                                 gCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGct                                                                    <  1:973654/140‑1 (MQ=255)                                                                 gCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGct                                                                    <  2:962479/140‑1 (MQ=255)                                                                     gACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTcc                                                                >  1:181148/1‑140 (MQ=255)                                                                      aCTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCt                                                               <  1:568828/140‑1 (MQ=255)                                                                                    tcttctTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGt                                                 >  2:903098/1‑140 (MQ=255)                                                                                       tctttcttCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTc                                              <  1:863248/140‑1 (MQ=255)                                                                                         tttcttCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTCaa                                            <  2:861176/140‑1 (MQ=255)                                                                                         tttcttCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTCaa                                            <  2:259662/140‑1 (MQ=255)                                                                                                     cGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTCAACCGTTAAGGCGt                                >  1:163708/1‑140 (MQ=255)                                                                                                     cGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTCAACCGTTAAGGCGt                                >  2:928428/1‑140 (MQ=255)                                                                                                       ccAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTGAACCGTTAAGGCGttt                              >  2:259860/1‑140 (MQ=255)                                                                                                       ccAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTCAACCGTTAAGGCGttt                              >  1:539622/1‑140 (MQ=255)                                                                                                       ccAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTCAACCGTTAAGGCGttt                              >  2:834093/1‑140 (MQ=255)                                                                                                       ccAGTTCGTACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTCAACCGTTAAGGCGttt                              >  1:33680/1‑140 (MQ=255)                                                                                                               tACTCTTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTCAACCGTTAAGGCGTTTGACGggca                      <  1:483681/140‑1 (MQ=255)                                                                                                                   ctTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTCAACCGTTAAGGCGTTTGACGggcaggca                  <  1:447378/140‑1 (MQ=255)                                                                                                                   ctTGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTCAACCGTTAAGGCGTTTGACGggcaggca                  <  2:3173/140‑1 (MQ=255)                                                                                                                     tGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTCAACCGTTAAGGCGTTTGACGGGCAGGCAtt                >  2:705327/1‑140 (MQ=255)                                                                                                                     tGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTCAACCGTTAAGGCGTTTGACGGGCAGGCAtt                >  1:939332/1‑140 (MQ=255)                                                                                                                     tGCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTCAACCGTTAAGGCGTTTGACGGGCAGGCAtt                >  1:725056/1‑140 (MQ=255)                                                                                                                      gCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTCAACCGTTAAGGCGTTTGACGGGCAGGCATTg               >  2:69946/1‑140 (MQ=255)                                                                                                                      gCGACAGTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTCAACCGTTAAGGCGTTTGACGGGCAGGCATTg               >  2:137770/1‑140 (MQ=255)                                                                                                                            gTTTGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTCAACCGTTAAGGCGTTTGACGGGCAGGCATTGACGCAg         >  1:710144/1‑140 (MQ=255)                                                                                                                              ttGATCGCCGCTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTCAACCGTTAAGGCGTTTGACGGGCAGGCATTGACGCAGGc       >  2:664512/1‑140 (MQ=255)                                                                                                                                   cgccgcTGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTCAACCGTTAAGGCGTTTGACGGGCAGGCATTGACGCAGGCCgcgc  <  2:551233/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      CGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTCTTCGCAGCGTCCACAGAAGATGCAGTGCCCAAGATTAAACTCCCAGGCAAGCTCTCCTGTGGCGAGGTCAGTTTCAACCGTTAAGGCGTTTGACGGGCAGGCATTGACGCAGGCCGCGC  >  NC_000913/2844356‑2844625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑