Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	247,673	A→G	T13A (ACA→GCA)	rayT →	REP‑associated tyrosine transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	247,673	0	A	G	100.0%	207.2 / NA	57	T13A (ACA→GCA)	rayT	REP‑associated tyrosine transposase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/21);  total (36/21)

CGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAACATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAGCGAGACAGGCCTTTTGAAATCAACGCCTGGGTCGTTTTGCCAG  >  NC_000913/247541‑247808                                                                                                                                     |                                                                                                                                        cGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGtt                                                                                                                                  >  1:648915/1‑140 (MQ=255)        gcgAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACg                                                                                                                           <  1:402616/140‑1 (MQ=255)               tttGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAAtt                                                                                                                    >  1:777676/1‑140 (MQ=255)                          cTTTCCCGATATAAAACAACCTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAAtcgt                                                                                                         >  1:24575/1‑140 (MQ=255)                                    ataAAACAACCTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCTTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAAc                                                                                               >  2:139681/1‑140 (MQ=255)                                     taAAACAACCTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACt                                                                                              <  2:336310/140‑1 (MQ=255)                                          caacCTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTCTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGa                                                                                         <  1:721581/140‑1 (MQ=255)                                           aacCTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGac                                                                                        <  1:383796/140‑1 (MQ=255)                                            acCTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGacc                                                                                       >  2:996011/1‑140 (MQ=255)                                             ccTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGacca                                                                                      >  2:937366/1‑140 (MQ=255)                                             ccTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGacca                                                                                      >  1:723355/1‑140 (MQ=255)                                             ccTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGacca                                                                                      >  2:592200/1‑140 (MQ=255)                                             ccTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGacca                                                                                      >  1:362311/1‑140 (MQ=255)                                             ccTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACAGCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGacca                                                                                      >  2:812159/1‑140 (MQ=255)                                              cTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGaccac                                                                                     <  1:801097/140‑1 (MQ=255)                                               tCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGaccacc                                                                                    <  2:648915/140‑1 (MQ=255)                                               tCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGaccacc                                                                                    <  2:777676/140‑1 (MQ=255)                                               tCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGaccacc                                                                                    <  1:937366/140‑1 (MQ=255)                                                 aTTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCa                                                                                  <  2:886647/140‑1 (MQ=255)                                                     ccAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGTAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTAc                                                                              <  1:440520/140‑1 (MQ=255)                                                       aaCCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCa                                                                            >  1:99768/1‑140 (MQ=255)                                                        aCCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAg                                                                           >  1:661526/1‑140 (MQ=255)                                                        aCCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAg                                                                           >  2:520350/1‑140 (MQ=255)                                                          ctttcctttTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGAt                                                                         >  1:268210/1‑140 (MQ=255)                                                          ctttcctttTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGAt                                                                         >  1:1803/1‑140 (MQ=255)                                                                    cttcttACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCAc                                                               >  2:701072/1‑140 (MQ=255)                                                                       cttACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGcc                                                            <  1:12375/140‑1 (MQ=255)                                                                       cttACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGcc                                                            <  1:212204/140‑1 (MQ=255)                                                                       cttACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGcc                                                            <  1:287735/140‑1 (MQ=255)                                                                         tACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCat                                                          >  2:219140/1‑140 (MQ=255)                                                                              ttcagaAAAAGGCGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGCCCACCCAGTACCAGATGGTCCGTCACGCCAttatta                                                     >  2:652723/4‑140 (MQ=255)                                                                              ttGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCAttatta                                                     >  1:920316/1‑140 (MQ=255)                                                                                 agaAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAg                                                  >  2:148985/1‑140 (MQ=255)                                                                                 agaAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAg                                                  <  1:493605/140‑1 (MQ=255)                                                                                 agaAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAg                                                  >  2:664235/1‑140 (MQ=255)                                                                                      aaGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAg                                             <  2:24575/140‑1 (MQ=255)                                                                                      aaGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAg                                             <  1:812159/140‑1 (MQ=255)                                                                                      aaGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAg                                             <  2:645058/140‑1 (MQ=255)                                                                                           gTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAGCgaga                                        >  2:329389/1‑140 (MQ=255)                                                                                           gTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAGCgaga                                        <  1:388086/140‑1 (MQ=255)                                                                                                             cgtcgtTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAGCGAGACAGGCCTTTTGAAATCaa                      >  2:991121/1‑140 (MQ=255)                                                                                                             cgtcgtTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAGCGAGACAGGCCTTTTGAAATCaa                      >  2:414512/1‑140 (MQ=255)                                                                                                             cgtcgtTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAGCGAGACAGGCCTTTTGAAATCaa                      >  2:677567/1‑140 (MQ=255)                                                                                                             cgtcgtTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGAAGCTCCGTCACGCCATTATTAAAGTTAAGCGAGACAGGCCTTTTGAAATCaa                      >  2:382329/1‑140 (MQ=255)                                                                                                              gtcgtTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAGCGAGACAGGCCTTTTGAAATCAAc                     >  2:939132/1‑140 (MQ=255)                                                                                                              gtcgtTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAGCGAGACAGGCCTTTTGAAATCAAc                     >  2:468343/1‑140 (MQ=255)                                                                                                              gtcgtTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAGCGAGACAGGCCTTTTGAAATCAAc                     >  2:122045/1‑140 (MQ=255)                                                                                                               tcgtTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGTCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAGCGAGACAGGCCTTTTGAAATCAACg                    >  1:240493/1‑140 (MQ=255)                                                                                                               tcgtTATTACATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAGCGAGACAGGCCTTTTGAAATCAACg                    >  2:779387/1‑140 (MQ=255)                                                                                                                  ttattaCATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAGCGAGACAGGCCTTTTGAAATCAACGCCt                 >  2:149021/1‑140 (MQ=255)                                                                                                                    attaCATCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAGCGAGACAGGCCTTTTGAAATCAACGCCTgg               <  1:329389/140‑1 (MQ=255)                                                                                                                         aTCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAGCGAGACAGGCCTTTTGAAATCAACGCCTGGGTCGt          >  2:611941/1‑140 (MQ=255)                                                                                                                          tCAAGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAGCGAGACAGGCCTTTTGAAATCAACGCCTGGGTCGtt         >  2:122297/1‑140 (MQ=255)                                                                                                                             aGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAGCGAGACAGGCCTTTTGAAATCAACGCCTGGGTCGTTTTg      >  1:127471/1‑140 (MQ=255)                                                                                                                             aGGGGGGAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAGCGAGACAGGCCTTTTGAAATCAACGCCTGGGTCGTTTTg      >  1:250893/1‑140 (MQ=255)                                                                                                                               gggggAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAGCGAGACAGGCCTTTTGAAATCAACGCCTGGGTCGTTTTGcc    >  1:915331/1‑140 (MQ=255)                                                                                                                               gggggAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAGCGAGACAGGCCTTTTGAAATCAACGCCTGGGTCGTTTTGcc    <  2:99768/140‑1 (MQ=255)                                                                                                                                 gggAGCATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAGCGAGACAGGCCTTTTGAAATCAACGCCTGGGTCGTTTTGCCag  <  2:920316/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        CGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAACCTTTCCTTTTCTTCTTACCGTTGAGAAAAAGGAGTCGCCATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAACATGGTTTTTCACGGTGAATTTACGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATTAAAGTTAAGCGAGACAGGCCTTTTGAAATCAACGCCTGGGTCGTTTTGCCAG  >  NC_000913/247541‑247808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑