Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,899,590	A→G	D228D (GAT→GAC)	ygcW ←	putative deoxygluconate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,899,590	0	A	G	100.0%	305.1 / NA	83	D228D (GAT→GAC)	ygcW	putative deoxygluconate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/40);  total (43/40)

TAATAAGAGAAAACAGAAACCACCGTTATGGGTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTATCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAATATTATATTGACCTAGTT  >  NC_000913/2899456‑2899726                                                                                                                                       |                                                                                                                                         taataaGAGAAAACAGAAACCACCGTTATGGGTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTgccgcc                                                                                                                                     >  2:213374/1‑140 (MQ=255)  aataaGAGAAAACAGAAACCACCGTTATGGGTTAGCGCACTAAATAACCGCCATCAACCACTAGTAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGccc                                                                                                                                    >  1:854837/1‑140 (MQ=255)  aataaGAGAAAACAGAAACCACCGTTATGGGTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGccc                                                                                                                                    >  2:465245/1‑140 (MQ=255)  aataaGAGAAAACAGAAACCACCGTTATGGGTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGccc                                                                                                                                    >  2:464376/1‑140 (MQ=255)  aataaGAGAAAACAGAAACCACCGTTATGGGTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGccc                                                                                                                                    >  1:548495/1‑140 (MQ=255)     aaGAGAAAACAGAAACCACCGTTATGGGTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCaa                                                                                                                                 >  2:540323/1‑140 (MQ=255)     aaGAGAAAACAGAAACCACCGTTATGGGTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCaa                                                                                                                                 <  1:678330/140‑1 (MQ=255)       gagaAAACAGAAACCACCGTTATGGGTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACg                                                                                                                               >  1:206918/1‑140 (MQ=255)       gagaAAACAGAAACCACCGTTATGGGTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACg                                                                                                                               >  2:293493/1‑140 (MQ=255)        agaAAACAGAAACCACCGTTATGGGTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACgg                                                                                                                              >  2:691895/1‑140 (MQ=255)         gaAAACAGAAACCACCGTTATGGGTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGt                                                                                                                             <  1:785374/140‑1 (MQ=255)             aCAGAAACCTCCGTTATGGGTTAGCGCTCTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGc                                                                                                                         <  1:510532/140‑1 (MQ=255)             aCAGAAACCACCGTTATGGGTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGc                                                                                                                         <  2:597824/140‑1 (MQ=255)             aCAGAAACCACCGTTATGGGTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGc                                                                                                                         <  2:620778/140‑1 (MQ=255)             aCAGAAACCACCGTTATGGGTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGc                                                                                                                         <  1:164137/140‑1 (MQ=255)              aaGAAACCGCCGTTATGGGTTAGCGCCCTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGcc                                                                                                                        <  1:462095/139‑1 (MQ=255)                 aaaCCACCGTTATGGGTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGa                                                                                                                     >  1:651572/1‑140 (MQ=255)                   accaccGTTATGGGTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAat                                                                                                                   <  1:672032/140‑1 (MQ=255)                         gTTATGGGTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCTGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATc                                                                                                             >  2:196015/1‑140 (MQ=255)                         gTTATGGGTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATc                                                                                                             >  1:292803/1‑140 (MQ=255)                          ttATGGGTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATATGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCa                                                                                                            <  1:542900/140‑1 (MQ=255)                             tGGGTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGa                                                                                                         >  1:144854/1‑140 (MQ=255)                               ggTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAAc                                                                                                       >  1:143728/1‑140 (MQ=255)                                 ttAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGANNGTCNCCCCAACGGTTTGCCGGAATATGATCAAGAAcgc                                                                                                     <  2:771590/140‑1 (MQ=255)                                  tAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAAcgcg                                                                                                    >  1:496472/1‑140 (MQ=255)                                  tAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAAcgcg                                                                                                    >  1:971766/1‑140 (MQ=255)                                  tAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAAcgcg                                                                                                    >  2:122497/1‑140 (MQ=255)                                  tAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAAcgcg                                                                                                    <  2:1021595/140‑1 (MQ=255)                                   aGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACgcgc                                                                                                   >  1:343485/1‑140 (MQ=255)                                   aGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACgcgc                                                                                                   >  1:350259/1‑140 (MQ=255)                                    gcgcACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCt                                                                                                  >  1:173411/1‑140 (MQ=255)                                         cTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTg                                                                                             >  2:567270/1‑140 (MQ=255)                                          tAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTgg                                                                                            >  1:646274/1‑140 (MQ=255)                                           aaaTAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGt                                                                                           <  1:701880/140‑1 (MQ=255)                                             aTAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGttt                                                                                         <  2:206918/140‑1 (MQ=255)                                             aTAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGttt                                                                                         <  2:233601/140‑1 (MQ=255)                                                 ccGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTgg                                                                                     >  1:507879/1‑140 (MQ=255)                                                    ccATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGAtt                                                                                  >  2:343444/1‑140 (MQ=255)                                                           ccACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCgt                                                                           >  2:469283/1‑140 (MQ=255)                                                            cACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCgtg                                                                          >  2:635510/1‑140 (MQ=255)                                                                 ataaATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGcc                                                                     >  1:334047/1‑140 (MQ=255)                                                                 ataaATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGcc                                                                     >  2:96318/1‑140 (MQ=255)                                                                  taaATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCa                                                                    <  2:292803/140‑1 (MQ=255)                                                                     aTGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCg                                                                 >  1:552485/1‑140 (MQ=255)                                                                      tGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGt                                                                >  1:634624/1‑140 (MQ=255)                                                                        cccGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTaa                                                              >  2:712012/1‑140 (MQ=255)                                                                         ccGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAat                                                             >  2:836987/1‑140 (MQ=255)                                                                            ttGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATc                                                          <  1:836987/140‑1 (MQ=255)                                                                            ttGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATc                                                          >  2:452906/1‑140 (MQ=255)                                                                            ttGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATc                                                          <  1:691895/140‑1 (MQ=255)                                                                                 aTAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTg                                                     <  1:213374/140‑1 (MQ=255)                                                                                     ttCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCata                                                 <  2:343485/140‑1 (MQ=255)                                                                                      tCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCataa                                                <  2:144854/140‑1 (MQ=255)                                                                                         aTGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAg                                             >  2:768207/1‑140 (MQ=255)                                                                                            ccGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCa                                          <  1:567270/140‑1 (MQ=255)                                                                                            ccGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCa                                          <  2:507879/140‑1 (MQ=255)                                                                                             cGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAg                                         >  1:110685/1‑140 (MQ=255)                                                                                                   tGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGa                                   <  1:469283/140‑1 (MQ=255)                                                                                                   tGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGa                                   <  1:196015/140‑1 (MQ=255)                                                                                                   tGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGa                                   <  1:122497/140‑1 (MQ=255)                                                                                                       aGGAATACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATAcc                               >  2:466096/1‑140 (MQ=255)                                                                                                          aaTACGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCAtt                            <  2:548495/140‑1 (MQ=255)                                                                                                             aCGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTAc                         <  1:635510/140‑1 (MQ=255)                                                                                                             aCGGCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTAc                         <  2:671478/140‑1 (MQ=255)                                                                                                               ggCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCt                       <  1:96318/140‑1 (MQ=255)                                                                                                               ggCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCt                       <  2:152206/140‑1 (MQ=255)                                                                                                               ggCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCt                       <  2:143728/140‑1 (MQ=255)                                                                                                               ggCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCt                       <  2:334047/140‑1 (MQ=255)                                                                                                               ggCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCt                       <  1:939326/140‑1 (MQ=255)                                                                                                               ggCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCt                       <  1:540323/140‑1 (MQ=255)                                                                                                               ggCTGCGCCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGATTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCt                       <  2:837097/140‑1 (MQ=255)                                                                                                                   gcgcCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAat                   <  2:552485/140‑1 (MQ=255)                                                                                                                   gcgcCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAat                   >  1:829453/1‑140 (MQ=255)                                                                                                                    cgcCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTTAata                  >  2:199206/1‑140 (MQ=255)                                                                                                                    cgcCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAata                  <  1:95435/140‑1 (MQ=255)                                                                                                                    cgcCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAata                  >  1:260512/1‑140 (MQ=255)                                                                                                                    cgcCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAata                  >  1:635906/1‑140 (MQ=255)                                                                                                                    cgcCCATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAata                  >  1:338870/1‑140 (MQ=255)                                                                                                                        cATTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAatattat              <  2:971766/140‑1 (MQ=255)                                                                                                                         aTTAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAAGGGCGATACCATTTACCTGAatattata             >  2:925163/1‑140 (MQ=255)                                                                                                                          ttAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAatattatat            <  1:691217/140‑1 (MQ=255)                                                                                                                          ttAAATCCTGAGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAatattatat            <  2:854837/140‑1 (MQ=255)                                                                                                                                    aGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAATATTATATTGACCTAGtt  >  1:734897/1‑140 (MQ=255)                                                                                                                                    aGTGTCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAATATTATATTGACCTAGtt  >  2:1023811/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                         TAATAAGAGAAAACAGAAACCACCGTTATGGGTTAGCGCACTAAATAACCGCCATCAACCACTAATAAATGCCCGTTGACATAATTCGATGCCGGACTTGCGAGGAATACGGCTGCGCCCATTAAATCCTGAGTATCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAATATTATATTGACCTAGTT  >  NC_000913/2899456‑2899726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑