Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,975,132	T→C	T295A (ACC→GCC)	aas ←	acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,975,132	0	T	C	100.0%	145.9 / NA	41	T295A (ACC→GCC)	aas	acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/24);  total (17/24)

ACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTT  >  NC_000913/2974996‑2975267                                                                                                                                         |                                                                                                                                        aCATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAg                                                                                                                                      <  2:354857/140‑1 (MQ=255)      tGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCtt                                                                                                                                 >  1:741719/1‑140 (MQ=255)       gCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCttt                                                                                                                                <  2:167395/140‑1 (MQ=255)        cTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGGTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCtttt                                                                                                                               >  2:388404/1‑140 (MQ=255)            tAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTAccc                                                                                                                           <  1:746302/140‑1 (MQ=255)             aaaTCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTAcccc                                                                                                                          >  1:189415/1‑140 (MQ=255)               aTCTTCCAGATAGACCCGGCGCCCCTGAGTGAGTTGCTCGGGCAGAGGCCAGATTTTGCCTTTATCGAGAAACTGGGGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCgg                                                                                                                        <  2:419438/140‑1 (MQ=255)               aTCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCgg                                                                                                                        <  2:355554/140‑1 (MQ=255)                  ttCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAg                                                                                                                     <  1:742044/140‑1 (MQ=255)                  ttCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGGTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAg                                                                                                                     >  2:471546/1‑140 (MQ=255)                   tCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAgt                                                                                                                    >  1:529704/1‑140 (MQ=255)                    ccAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAgtg                                                                                                                   <  2:877182/140‑1 (MQ=255)                    ccAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAgtg                                                                                                                   <  1:814840/140‑1 (MQ=255)                    ccAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAgtg                                                                                                                   <  1:404708/140‑1 (MQ=255)                        aTAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGt                                                                                                               >  2:310676/1‑140 (MQ=255)                            aCCCAGCGCACCTGAGTAAGTTGCTCCGACAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAAAAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTtcat                                                                                                           >  1:770767/1‑140 (MQ=255)                                  cgcACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGc                                                                                                     >  1:170393/1‑140 (MQ=255)                                        tGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCACCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCAt                                                                                               <  2:345872/140‑1 (MQ=255)                                                 tGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGGGAAGATGGTTTTGATTTTAGCCGCCGTAATAGCACTGGGCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCCGCGCCg                                                                                      >  2:299679/1‑140 (MQ=255)                                                   cTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACggg                                                                                    >  2:663959/1‑140 (MQ=255)                                                     ccGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCg                                                                                  >  1:548079/1‑140 (MQ=255)                                                     ccGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCg                                                                                  <  2:189415/140‑1 (MQ=255)                                                             tGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGcccc                                                                          >  2:910863/1‑140 (MQ=255)                                                             tGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGcccc                                                                          >  1:791507/1‑140 (MQ=255)                                                                     tttGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCa                                                                  <  2:1011150/140‑1 (MQ=255)                                                                        gCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTCATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCTATGGCCCCAAAAATCACTg                                                               >  1:991981/1‑140 (MQ=255)                                                                               tCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACt                                                        >  2:745763/1‑140 (MQ=255)                                                                               tCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACt                                                        <  1:471546/140‑1 (MQ=255)                                                                                    aaaCTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGc                                                   <  2:840752/140‑1 (MQ=255)                                                                                     aaCTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGcc                                                  >  2:756446/1‑140 (MQ=255)                                                                                         ggCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCa                                              <  2:33430/140‑1 (MQ=255)                                                                                              gAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTggg                                         <  1:927343/140‑1 (MQ=255)                                                                                               aaGTGAAGATGTTTTTGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGgc                                        <  2:741719/140‑1 (MQ=255)                                                                                                           ttttCATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCTATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGACg                            <  2:991981/140‑1 (MQ=255)                                                                                                            tttGATTTCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGa                           <  1:388404/140‑1 (MQ=255)                                                                                                                 tttCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGt                      <  1:387658/140‑1 (MQ=255)                                                                                                                   tCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTc                    <  2:464033/140‑1 (MQ=255)                                                                                                                   tCAGCCGCCGTAATAGCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTc                    <  1:910863/140‑1 (MQ=255)                                                                                                                                  gCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTa     <  1:956269/140‑1 (MQ=255)                                                                                                                                  gCACTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTa     <  2:74583/140‑1 (MQ=255)                                                                                                                                     cTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTAttt  >  1:120941/1‑140 (MQ=255)                                                                                                                                     cTGGCCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTAttt  >  1:103304/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        ACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTT  >  NC_000913/2974996‑2975267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑