Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,977,601	G→A	E335K (GAG→AAG)	galR →	DNA‑binding transcriptional dual regulator GalR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,977,601	0	G	A	100.0%	207.9 / NA	60	E335K (GAG→AAG)	galR	DNA‑binding transcriptional dual regulator GalR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/32);  total (28/32)

TACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGG  >  NC_000913/2977466‑2977736                                                                                                                                        |                                                                                                                                        tACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGc                                                                                                                                     <  2:68471/140‑1 (MQ=255)   cccAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTAGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCaa                                                                                                                                   >  1:163873/1‑140 (MQ=255)      aaTCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGtc                                                                                                                                >  1:108464/1‑140 (MQ=255)      aaTCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGtc                                                                                                                                <  2:872742/140‑1 (MQ=255)      aaTCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGtc                                                                                                                                <  1:255836/140‑1 (MQ=255)      aaTCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGtc                                                                                                                                <  2:294466/140‑1 (MQ=255)           tGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTcatcat                                                                                                                           >  2:940595/1‑140 (MQ=255)            gACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATg                                                                                                                          <  1:710159/140‑1 (MQ=255)            gACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATg                                                                                                                          <  1:654647/140‑1 (MQ=255)             aCGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGc                                                                                                                         >  2:726894/1‑140 (MQ=255)                aTGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAAc                                                                                                                      <  1:424824/140‑1 (MQ=255)                 tGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAAcc                                                                                                                     >  2:431647/1‑140 (MQ=255)                   gCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCACCTCCGTCGCTGAAGGCAAGTCATCATGCAACCAg                                                                                                                   <  1:259240/140‑1 (MQ=255)                   gCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAg                                                                                                                   <  2:951945/140‑1 (MQ=255)                             cTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCg                                                                                                         <  2:108464/140‑1 (MQ=255)                               gCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCa                                                                                                       <  1:280684/140‑1 (MQ=255)                                ccGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAg                                                                                                      >  2:602552/1‑140 (MQ=255)                                 cGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGt                                                                                                     >  1:471620/1‑140 (MQ=255)                                        gCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCa                                                                                              <  2:794582/140‑1 (MQ=255)                                             ggCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTcc                                                                                         >  1:589237/1‑140 (MQ=255)                                                gcTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAgc                                                                                      <  2:556666/140‑1 (MQ=255)                                                  tGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAgcgc                                                                                    >  2:106873/1‑140 (MQ=255)                                                      ggATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGt                                                                                <  1:726894/140‑1 (MQ=255)                                                                ccTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGa                                                                      <  1:534888/140‑1 (MQ=255)                                                                ccTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGa                                                                      <  1:607200/140‑1 (MQ=255)                                                                 ctctCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGAt                                                                     >  1:732932/1‑140 (MQ=255)                                                                 ctctCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGAt                                                                     >  1:215368/1‑140 (MQ=255)                                                                        ggAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGAAATTCTTCGATGGTCTgg                                                              <  2:603754/140‑1 (MQ=255)                                                                            aTCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGAc                                                          <  2:732932/140‑1 (MQ=255)                                                                              cACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACgg                                                        >  2:873293/1‑140 (MQ=255)                                                                              cACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACgg                                                        >  2:80827/1‑140 (MQ=255)                                                                              cACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACgg                                                        >  2:762275/1‑140 (MQ=255)                                                                              cACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACgg                                                        >  1:568586/1‑140 (MQ=255)                                                                                       cTTTAGTCCGACGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAAc                                               <  2:163873/140‑1 (MQ=255)                                                                                            gTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCgcgc                                          <  2:281132/140‑1 (MQ=255)                                                                                             tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGcc                                         >  1:404184/1‑140 (MQ=255)                                                                                             tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGcc                                         <  1:106873/140‑1 (MQ=255)                                                                                                 aCGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGGGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGAc                                     >  2:221857/1‑140 (MQ=255)                                                                                                 aCGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGAc                                     >  2:653568/1‑140 (MQ=255)                                                                                                 aCGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGAc                                     >  2:679155/1‑140 (MQ=255)                                                                                                 aCGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGAc                                     <  1:189849/140‑1 (MQ=255)                                                                                                 aCGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGAc                                     >  2:30395/1‑140 (MQ=255)                                                                                                  cGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGAcc                                    >  1:497463/1‑140 (MQ=255)                                                                                                   gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCa                                   <  1:270705/140‑1 (MQ=255)                                                                                                       gTACGTCGTCATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTAt                               >  1:177455/1‑140 (MQ=255)                                                                                                              gtcATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAg                        <  2:215368/140‑1 (MQ=255)                                                                                                               tcATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGa                       >  2:239596/1‑140 (MQ=255)                                                                                                               tcATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGa                       >  2:119751/1‑140 (MQ=255)                                                                                                               tcATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGa                       >  1:469009/1‑140 (MQ=255)                                                                                                                cATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGaa                      <  2:474581/140‑1 (MQ=255)                                                                                                                cATTCAGTGTCAACTCCGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGaa                      <  2:471620/140‑1 (MQ=255)                                                                                                                               ccGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCg       <  1:108586/140‑1 (MQ=255)                                                                                                                                cGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCgg      <  2:1008783/140‑1 (MQ=255)                                                                                                                                cGTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCgg      <  1:873293/140‑1 (MQ=255)                                                                                                                                 gTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGa     >  1:715422/1‑140 (MQ=255)                                                                                                                                 gTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGa     >  2:296116/1‑140 (MQ=255)                                                                                                                                 gTCGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGa     >  1:617898/1‑140 (MQ=255)                                                                                                                                   cGCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACg   <  2:1019016/140‑1 (MQ=255)                                                                                                                                    gCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACgg  <  1:792112/140‑1 (MQ=255)                                                                                                                                    gCTGAAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACgg  <  1:780883/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TACCCAATCGTGACGATGGCGACCCAGGCTGCCGAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGG  >  NC_000913/2977466‑2977736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑