Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,019,754	T→C	G198G (GGT→GGC)	ssnA →	putative aminohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,019,754	0	T	C	100.0%	200.6 / NA	54	G198G (GGT→GGC)	ssnA	putative aminohydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/27);  total (27/27)

GTTTTGAAACTACTGACCGTAACAACGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGTGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGTTTGCATATTCACGCTGCGGAAGACCTTTACGACGTTTCCTACAGTCACCACTGGTACG  >  NC_000913/3019618‑3019887                                                                                                                                         |                                                                                                                                      tttttGAAACTACTGACCGTAACAACGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCt                                                                                                                                    <  2:755251/139‑1 (MQ=255)  ttttGAAACTACTGACCGTAACAACGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTc                                                                                                                                   >  1:390140/1‑140 (MQ=255)    ttGAAACTACTGACCGTAACAACGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCAc                                                                                                                                 <  1:80134/140‑1 (MQ=255)      gAAACTACTGACCGTAACAACGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCAcgc                                                                                                                               <  1:812558/140‑1 (MQ=255)            actGACCGTAACAACGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGtt                                                                                                                         >  1:660968/1‑140 (MQ=255)            actGACCGTAACAACGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGtt                                                                                                                         >  1:817309/1‑140 (MQ=255)            actGACCGTAACAACGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGtt                                                                                                                         >  1:331978/1‑140 (MQ=255)                  cGTAACAACGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGt                                                                                                                   >  2:131777/1‑140 (MQ=255)                          cGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATg                                                                                                           <  1:583616/140‑1 (MQ=255)                          cGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATg                                                                                                           >  2:489886/1‑140 (MQ=255)                          cGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATg                                                                                                           >  2:563972/1‑140 (MQ=255)                           ggCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGc                                                                                                          <  1:965553/140‑1 (MQ=255)                             cATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCg                                                                                                        <  1:250777/140‑1 (MQ=255)                             cATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCg                                                                                                        >  1:82227/1‑140 (MQ=255)                              aTCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCgg                                                                                                       <  1:697435/140‑1 (MQ=255)                               tCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGt                                                                                                      >  1:941220/1‑140 (MQ=255)                               tCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGt                                                                                                      >  1:992199/1‑140 (MQ=255)                               tCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGt                                                                                                      >  1:133663/1‑140 (MQ=255)                                  aaGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTg                                                                                                   >  1:998532/1‑140 (MQ=255)                                        tGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGAtg                                                                                             <  1:193133/140‑1 (MQ=255)                                         gCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGAtgc                                                                                            >  2:635015/1‑140 (MQ=255)                                            ggAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGTACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGAtgctgc                                                                                         >  2:764198/1‑140 (MQ=255)                                                 gtgtAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGaa                                                                                    >  1:628177/1‑140 (MQ=255)                                                            aaCATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGGCTGGAGATGCTGCGTGAAGCCGTGAAAGc                                                                         >  2:522029/1‑140 (MQ=255)                                                                tCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCcaca                                                                     >  1:647398/1‑140 (MQ=255)                                                                    tttCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGcc                                                                 <  2:331978/140‑1 (MQ=255)                                                                    tttCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGcc                                                                 <  2:954128/140‑1 (MQ=255)                                                                      tCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGt                                                               <  1:790537/140‑1 (MQ=255)                                                                        gCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTgg                                                             <  2:817309/140‑1 (MQ=255)                                                                        ccccGTTTAATTGATGAGGCGAAGAAGGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTgg                                                             <  2:660968/139‑1 (MQ=255)                                                                         cccGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGt                                                            <  1:393480/140‑1 (MQ=255)                                                                          ccGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGtt                                                           <  1:489886/140‑1 (MQ=255)                                                                           cGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGttt                                                          >  2:235743/1‑140 (MQ=255)                                                                            gTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGTTTg                                                         <  2:745573/140‑1 (MQ=255)                                                                                      tgaGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGTTTGCATATTCACg                                               <  1:816737/140‑1 (MQ=255)                                                                                      tgaGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCAGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGTTTGCATATTCACg                                               <  1:239081/140‑1 (MQ=255)                                                                                       gaGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGTTTGCATATTCACGc                                              <  2:941220/140‑1 (MQ=255)                                                                                          gCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGt                                                            >  2:869243/1‑123 (MQ=255)                                                                                          gCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGt                                                            <  1:869243/123‑1 (MQ=255)                                                                                              agaaAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGTTTGCATATTCACGCTGCGGaa                                       <  1:335767/140‑1 (MQ=255)                                                                                                  aGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGTTTGCATATTCACGCTGCGGAAGAcc                                   >  1:860545/1‑140 (MQ=255)                                                                                                  aGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGTTTGCATATTCACGCTGCGGAAGAcc                                   >  1:648961/1‑140 (MQ=255)                                                                                                    cGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGTTTGCATATTCACGCTGCGGAAGACCtt                                 >  2:374861/1‑140 (MQ=255)                                                                                                    cGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGTTTGCATATTCACGCTGCGGAAGACCtt                                 >  1:929621/1‑140 (MQ=255)                                                                                                    cGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGTTTGCATATTCACGCTGCGGAAGACCtt                                 >  1:104203/1‑140 (MQ=255)                                                                                                    cGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTAACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGTTTGCATATTCACGCTGCGGAAGACCtt                                 >  2:792017/1‑140 (MQ=255)                                                                                                        aaGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGTTTGCATATTCACGCTGCGGAAGACCTTTacg                             <  1:235743/140‑1 (MQ=255)                                                                                                        aaGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGTTTGCATATTCACGCTGCGGAAGACCTTTacg                             <  2:992199/140‑1 (MQ=255)                                                                                                        aaGCGAGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGTTTGCATATTCACGCTGCGGAAGACCTTTacg                             <  2:82227/140‑1 (MQ=255)                                                                                                          gCGAGCCGTATCTGGTGGCAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGTTTGCATATTCACGCTGCGGAAGACCTTTacgac                           <  2:573089/140‑1 (MQ=255)                                                                                                             aGCCGTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGGTTGCATATTCACGCTGCGGAAGACCTTTACGACGtt                        >  1:863998/1‑140 (MQ=255)                                                                                                                 gTATCTGGTGGAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGTTTGCATATTCACGCTGCGGAAGACCTTTACGACGTTTCCt                    <  2:998532/140‑1 (MQ=255)                                                                                                                      tggtggAAGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGTTTGCATATTCACGCTGCGGAAGACCTTTACGACGTTTCCTACAGt               <  1:141593/140‑1 (MQ=255)                                                                                                                            aaGCACATATCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGTTTGCATATTCACGCTGCGGAAGACCTTTACGACGTTTCCTACAGTcaccac         >  1:720578/1‑140 (MQ=255)                                                                                                                                   tatCGGCGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGTTTGCATATTCACGCTGCGGAAGACCTTTACGACGTTTCCTACAGTCACCACTGGTACg  >  1:384441/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      GTTTTGAAACTACTGACCGTAACAACGGCATCAAAGAGTTGCAGGAAGGTGTAGAAGAAAACATCCGTTTCGCCCGTTTGATTGATGAGGCGAAGAAAGCGACAAGCGAGCCGTATCTGGTGGAAGCACATATCGGTGCTCACGCGCCGTTTACCGTGCCGGATGCCGGTCTGGAGATGCTGCGTGAAGCCGTGAAAGCCACAGGCCGTGGTTTGCATATTCACGCTGCGGAAGACCTTTACGACGTTTCCTACAGTCACCACTGGTACG  >  NC_000913/3019618‑3019887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑