Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	369,972	(G)5→6	coding (1362/1665 nt)	mhpA →	putative 3‑(3‑hydroxyphenyl)propanoate/3‑hydroxycinnamate hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	369,967	1	.	G	100.0%	137.7 / NA	37	W453G (TGG→GGG)	mhpA	putative 3‑(3‑hydroxyphenyl)propanoate/3‑hydroxycinnamate hydroxylase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (15/22);  total (15/22)

GGGCGAAGCGAAGCACTCTCCGGTCGGCAAGATGTTTATTCAGCCGAAAGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGT‑GGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCATTCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGT  >  NC_000913/369831‑370085                                                                                                                                          |                                                                                                                       gggCGAAGCGAAGCACTCTCCGGTCGGCAAGATGTTTATTCAGCCGAAAGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGT‑ggg                                                                                                                     >  1:1416145/1‑140 (MQ=255)   gcgaagcgaaGCACTCTCCGGTCGGCAAGATGTTTATTCAGCCGAAAGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGT‑ggggg                                                                                                                   >  2:1397807/1‑140 (MQ=255)   gcgaagcgaaGCACTCTCCGGTCGGCAAGATGTTTATTCAGCCGAAAGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGT‑ggggg                                                                                                                   >  2:330274/1‑140 (MQ=255)            agcACTCTCCGGTCGGCAAGATGTTTATTCAGCCGAAAGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGcga                                                                                                           >  1:604108/1‑140 (MQ=255)            agcACTCTCCGGTCGGCAAGATGTTTATTCAGCCGAAAGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGcga                                                                                                           >  1:434127/1‑140 (MQ=255)                        tCGGCAAGATGTTTATTCAGCCGAAAGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCca                                                                                               >  1:222439/1‑140 (MQ=255)                             aaGATGTTTATTCAGCCGAAAGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGt                                                                                          >  1:549958/1‑140 (MQ=255)                               gATGTTTATTCAGCCGAAAGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTgg                                                                                        >  2:886732/1‑140 (MQ=255)                                 tGTTTATTCAGCCGAAAGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGgcg                                                                                      <  1:329236/140‑1 (MQ=255)                                  gTTTATTCAGCCGAAAGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGgcgc                                                                                     >  2:1323327/1‑140 (MQ=255)                                    ttATTCAGCCGAAAGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGgcgcgc                                                                                   <  2:469873/140‑1 (MQ=255)                                           gCCGAAAGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGc                                                                            >  1:273754/1‑140 (MQ=255)                                           gCCGAAAGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGc                                                                            >  2:1439690/1‑140 (MQ=255)                                            ccGAAAGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGca                                                                           <  1:315079/140‑1 (MQ=255)                                            ccGAAAGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGca                                                                           >  2:694158/1‑140 (MQ=255)                                            ccGAAAGTCACGCTGGAAAACGGCGACGTGACGCCGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGca                                                                           >  2:809492/1‑140 (MQ=255)                                                aaGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACg                                                                       <  2:20461/140‑1 (MQ=255)                                                aaGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACg                                                                       <  1:942693/140‑1 (MQ=255)                                                aaGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACg                                                                       <  2:434127/140‑1 (MQ=255)                                                aaGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACg                                                                       <  2:1282486/140‑1 (MQ=255)                                                 aGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGc                                                                      <  1:1397807/140‑1 (MQ=255)                                                  gTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCt                                                                     <  2:1416145/140‑1 (MQ=255)                                                   tCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCtt                                                                    <  1:169012/140‑1 (MQ=255)                                                                    gACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCATTCAGGTGGTGCCgg                                                   <  2:249697/140‑1 (MQ=255)                                                                       gTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCATTCAGGTGGTGCCGGAAg                                                <  2:679315/140‑1 (MQ=255)                                                                              cgctCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCATTCAGGTGGTGCCGGAAGTGCAAAt                                         <  1:809492/139‑1 (MQ=255)                                                                                 tCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCATTCAGGTGGTGCCGGAAGTGCAAATTCa                                      >  2:1188552/1‑140 (MQ=255)                                                                                   gATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCATTCAGGTGGTGCCGGAAGTGCAAATTCATa                                    <  2:222439/140‑1 (MQ=255)                                                                                      aaCGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCATTCAGGTGGTGCCGGAAGTGCAAATTCATACCg                                 <  1:886732/140‑1 (MQ=255)                                                                                       aCGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCATTCAGGTGGTGCCGGAAGTGCAAATTCATACCGc                                <  2:1052092/140‑1 (MQ=255)                                                                                          cgATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCATTCAGGTGGTGCCGGAAGTGCAAATTCATACCGcaca                             >  1:73176/1‑140 (MQ=255)                                                                                             tCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCATTCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGa                          >  1:983033/1‑140 (MQ=255)                                                                                                     aaCTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCATTCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCacg                  <  1:1323327/140‑1 (MQ=255)                                                                                                      aCTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCATTCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCacga                 <  1:1197182/140‑1 (MQ=255)                                                                                                        ttCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCATTCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCacgacg               <  2:198578/140‑1 (MQ=255)                                                                                                         tCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCATTCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACgg              <  1:248295/140‑1 (MQ=255)                                                                                                          cgcgGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCATTCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGc             >  1:887255/1‑140 (MQ=255)                                                                                                           gcgGTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCATTCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCg            >  1:566715/1‑140 (MQ=255)                                                                                                             ggTAATTGGCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCATTCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCGta          <  2:273754/140‑1 (MQ=255)                                                                                                                     gCTGGGGATGCAATCCACTGTGGGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCATTCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGt  <  1:1188552/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                       GGGCGAAGCGAAGCACTCTCCGGTCGGCAAGATGTTTATTCAGCCGAAAGTCACGCTGGAAAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGT‑GGGGGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGGGCACACGCTTCATTCAGGTGGTGCCGGAAGTGCAAATTCATACCGCACAGGATAACCACGACGGCGTACTACGCGT  >  NC_000913/369831‑370085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑