Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,368,924	A→G	L87L (TTA→TTG)	fxsA →	protein FxsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,368,924	0	A	G	100.0%	303.8 / NA	82	L87L (TTA→TTG)	fxsA	protein FxsA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/37);  total (45/37)

TATGTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTACCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGG  >  NC_000913/4368792‑4369058                                                                                                                                     |                                                                                                                                       tATGTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGTTGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCt                                                                                                                                 <  2:170798/140‑1 (MQ=255) tATGTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCt                                                                                                                                 >  1:589859/1‑140 (MQ=255)   tGTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGGTTGCTGCTTTTATTGCCGGGCttt                                                                                                                               >  2:1162445/1‑140 (MQ=255)    gTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCtttt                                                                                                                              >  1:585845/1‑140 (MQ=255)        cTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTAc                                                                                                                          >  1:1122245/1‑140 (MQ=255)        cTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGGGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTAc                                                                                                                          >  2:1253195/1‑140 (MQ=255)         tGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTAcc                                                                                                                         >  1:1013579/1‑140 (MQ=255)          ggTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCg                                                                                                                        <  2:1358528/140‑1 (MQ=255)           gTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGa                                                                                                                       <  2:472895/140‑1 (MQ=255)           gTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGa                                                                                                                       <  2:574137/140‑1 (MQ=255)              cGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGAcct                                                                                                                    >  2:1184076/1‑138 (MQ=255)              cGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACtt                                                                                                                    >  1:1485692/1‑140 (MQ=255)                tAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTTCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTcc                                                                                                                  >  1:1361265/1‑140 (MQ=255)                 aaCCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCt                                                                                                                 <  1:1468063/140‑1 (MQ=255)                 aaCCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGGGAAAACCCAGCGGGGGAGATTATTAAAAGGGTTTCGCGGACTATTGCTGGTTTGCGGGTTTTTTTGCCGGGCTTTTTTACCGACTTccc                                                                                                                 >  2:957452/1‑139 (MQ=255)                  aCCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTc                                                                                                                <  2:826980/140‑1 (MQ=255)                   ccAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCg                                                                                                               <  2:589859/140‑1 (MQ=255)                      gggCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGGTGATTTAAAATGGTTCCCTGATCATTGGTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCCACTTcccccggg                                                                                                            >  2:856005/1‑134 (MQ=255)                      gggCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCt                                                                                                                 <  2:1493458/135‑1 (MQ=255)                      gggCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGtc                                                                                                            <  2:1260551/140‑1 (MQ=255)                      gggCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGGTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCt                                                                                                                 >  1:1493458/1‑135 (MQ=255)                       ggCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACTGACTTCCTCGGtct                                                                                                           <  1:1463816/140‑1 (MQ=255)                       ggCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGtct                                                                                                           <  2:973986/140‑1 (MQ=255)                       ggCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGtct                                                                                                           >  1:1181798/1‑140 (MQ=255)                       ggCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGtct                                                                                                           >  2:1221863/1‑140 (MQ=255)                       ggCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGGTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGgct                                                                                                           >  2:1196872/1‑140 (MQ=255)                         cTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGtcttc                                                                                                         <  1:194133/140‑1 (MQ=255)                          tttAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGtcttct                                                                                                        <  1:167658/140‑1 (MQ=255)                          tttAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGtcttct                                                                                                        <  1:1162445/140‑1 (MQ=255)                            tAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTTAAAACCCAGCCGGGGAGATGTTTAAAAACGTTTCCCCGCACATTGCTGGTTTTCCGCTTTTAATGCCGGGGTTTTTTACCGACCTCCTCGGgcctctgc                                                                                                      >  2:877642/1‑138 (MQ=255)                            tAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTAc                                                                                                      >  2:20717/1‑140 (MQ=255)                                 aTTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGAGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGGTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTa                                                                                                 >  1:1484729/1‑140 (MQ=255)                                        gCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTgccgc                                                                                          <  2:1223563/140‑1 (MQ=255)                                        gCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTgccgc                                                                                          <  2:332401/140‑1 (MQ=255)                                         cTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTgccgcc                                                                                         <  1:251731/140‑1 (MQ=255)                                         cTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGAGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGATTTTTTACCGCATTCCTCGGTCTTCTACTTTTATTgccgcc                                                                                         >  2:137426/1‑140 (MQ=255)                                          tGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTGCTACTTTTATTGccgccg                                                                                        <  1:1220968/140‑1 (MQ=255)                                              gcaggaAAAAAAGGCGGCGGGTTGAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTTTTGCCGCCGGTGc                                                                                    <  1:957452/140‑1 (MQ=255)                                                agcaAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCaa                                                                                  <  2:667894/140‑1 (MQ=255)                                                agcaAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCaa                                                                                  <  1:1060216/140‑1 (MQ=255)                                                agcaAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGATGCaa                                                                                  <  2:843563/140‑1 (MQ=255)                                                 gcaAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCaaa                                                                                 >  1:160919/1‑140 (MQ=255)                                                  caAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCaaaa                                                                                >  1:378832/1‑140 (MQ=255)                                                    aaaaaTGGCGGCGGGTGAAAACCCTGCGGCGGAGATGATTAAAAGAGTTTCGCCGACCATTGCTGGATTGCTGCCTTTATTGCCCGGCCTTTTTCTCCACCTCCTCCGGCTTCTAATTTTATTGCCGCCCGTGCAAAAGc                                                                              >  2:41483/1‑140 (MQ=255)                                                       aaTGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATc                                                                           <  1:302299/140‑1 (MQ=255)                                                        aTGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCt                                                                          <  2:468163/140‑1 (MQ=255)                                                          ggcggcGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGa                                                                        <  1:1221863/140‑1 (MQ=255)                                                            cggcggGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACa                                                                      >  1:330090/1‑140 (MQ=255)                                                            cggcggGTGAAAACCCAGCGGCGGAGATGATTAAAAGGGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGCCTTCTACTTTTATTGCCGCCGGTGCAAAAGCAGCTGACa                                                                      >  2:1101736/1‑140 (MQ=255)                                                               cggGTGAAACCCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTg                                                                   <  1:197811/140‑1 (MQ=255)                                                                      aaaCCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGa                                                            <  1:494513/140‑1 (MQ=255)                                                                       aaCCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGAt                                                           >  2:408020/1‑140 (MQ=255)                                                                        aCCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATg                                                          >  2:1213549/1‑140 (MQ=255)                                                                           cAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCg                                                       >  1:323454/1‑140 (MQ=255)                                                                            aGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGc                                                      >  1:1163790/1‑140 (MQ=255)                                                                            aGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGc                                                      >  1:308388/1‑140 (MQ=255)                                                                                 cggAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTg                                                 >  1:222214/1‑140 (MQ=255)                                                                                   gagaTGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTgcg                                               <  1:877642/140‑1 (MQ=255)                                                                                   gagaTGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTgcg                                               <  1:283607/140‑1 (MQ=255)                                                                                      atgatTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCtt                                            <  1:1225822/140‑1 (MQ=255)                                                                                        gatTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCtttt                                          <  1:137426/140‑1 (MQ=255)                                                                                          ttAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTtct                                        <  2:1256542/140‑1 (MQ=255)                                                                                           tAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTtctc                                       >  2:1524052/1‑140 (MQ=255)                                                                                             aaaGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGc                                     >  1:1347807/1‑140 (MQ=255)                                                                                                   tttCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCt                               >  1:526491/1‑140 (MQ=255)                                                                                                   tttCGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCt                               >  1:997735/1‑140 (MQ=255)                                                                                                      cGCTGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTggc                            >  1:396496/1‑140 (MQ=255)                                                                                                         tGATCATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTggcggc                         <  1:1144282/140‑1 (MQ=255)                                                                                                             cATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGtt                     >  2:216852/1‑140 (MQ=255)                                                                                                             cATTGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGtt                     >  2:1062653/1‑140 (MQ=255)                                                                                                               ttGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGtttt                   <  2:893735/140‑1 (MQ=255)                                                                                                                tGCTGGTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTa                  >  1:1516699/1‑140 (MQ=255)                                                                                                                     gTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGcc             >  2:1821/1‑140 (MQ=255)                                                                                                                     gTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGcc             <  1:848064/140‑1 (MQ=255)                                                                                                                     gTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGcc             >  2:1125369/1‑140 (MQ=255)                                                                                                                     gTTTGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGcc             >  2:830079/1‑140 (MQ=255)                                                                                                                       ttGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCgg           >  1:691646/1‑140 (MQ=255)                                                                                                                       ttGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCgg           >  1:296678/1‑140 (MQ=255)                                                                                                                       ttGCTGCTTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCgg           >  1:128147/1‑140 (MQ=255)                                                                                                                          ctgctTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGTGCTTTTCTCGGATGCCTGGCGGCGGTTTTAGCGCCGGGAc        >  1:977630/1‑140 (MQ=255)                                                                                                                          ctgctTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGAc        >  1:578901/1‑140 (MQ=255)                                                                                                                          ctgctTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGAc        >  1:732447/1‑140 (MQ=255)                                                                                                                          ctgctTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGAc        >  1:126891/1‑140 (MQ=255)                                                                                                                          ctgctTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGAc        >  2:802637/1‑140 (MQ=255)                                                                                                                          ctgctTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGAc        >  1:214987/1‑140 (MQ=255)                                                                                                                          ctgctTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGAc        >  2:203252/1‑140 (MQ=255)                                                                                                                           tgctTTTATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGAcc       >  2:800619/1‑140 (MQ=255)                                                                                                                              ttttATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGt    <  1:490117/140‑1 (MQ=255)                                                                                                                              ttttATTGCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGt    <  2:1516699/140‑1 (MQ=255)                                                                                                                                ttattgCCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTgg  <  2:1163790/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       TATGTCACTGGTACGTAACCAGGGCTTTAAGAATTTCGTGCTGATGCAGCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCTGGTTTGCTGCTTTTATTACCGGGCTTTTTTACCGACTTCCTCGGTCTTCTACTTTTATTGCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGCATGCCTGGCGGCGGTTTTAGCGCCGGGACCGGTGG  >  NC_000913/4368792‑4369058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑