Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,565,915	A→G	intergenic (‑65/+51)	yjiM ← / ← yjiN	putative dehydratase subunit/DUF445 domain‑containing protein YjiN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,565,915	0	A	G	100.0%	256.9 / NA	68	intergenic (‑65/+51)	yjiM/yjiN	putative dehydratase subunit/DUF445 domain‑containing protein YjiN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/26);  total (42/26)

GGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTA  >  NC_000913/4565779‑4566047                                                                                                                                         |                                                                                                                                     ggTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTgg                                                                                                                                   <  1:447263/140‑1 (MQ=255) ggTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTgg                                                                                                                                   <  1:178339/140‑1 (MQ=255)  gTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGGAGATCGGGGACAAGGGACATGATTTTTCTTCCCCGTACCACGGGAGAGAAAGAAAAGAGGCCACATTATTACCATTCTTTTTGTGTggg                                                                                                                                  >  2:1280569/1‑139 (MQ=255)   tGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGtt                                                                                                                                 >  1:1446548/1‑140 (MQ=255)                 tGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGAtctctct                                                                                                                   <  2:1526028/140‑1 (MQ=255)                       gCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGt                                                                                                             <  2:1056767/140‑1 (MQ=255)                        cTTCAGAGAACTGATCGAAAATGGCGGGGAGATCGGGGGCAAAGGACATGATTTTCCTTCCCCCTACCACGGGAGAGAATGAAAAAAGGGCACATTATTACCATTCTTTTTGTGTGGTTTTTTTGATCTCTCTCGCGGtt                                                                                                            >  2:1072573/1‑140 (MQ=255)                         ttCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTa                                                                                                           <  1:451508/140‑1 (MQ=255)                                 aCTGATCGAAAATGGCGTGTAGATCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTa                                                                                                   >  2:1385008/1‑140 (MQ=255)                                  cTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAg                                                                                                  <  1:500612/140‑1 (MQ=255)                                     aTCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGATTTTTCTTCCCCGTACCACGGGGGATAATGAAAAAAGGCCACCTTATTTCCATTCTTTTTGTGTGGGTTATTTTATCTCTCTCGCGGGTAGCCACTTTGttt                                                                                               >  2:1445127/1‑140 (MQ=255)                                     aTCGAAAATGGCGGGTAGATCGGGGACAAGTGACATGATTTTTCTTCCCCCTACCACGGGGGATAATGAAAAGAGGGCACATTATTATCATTCTTTTAGTTTGGGTTTTTTTATATCTCTCCCCGGTAGCCACTTAGttt                                                                                               >  2:1453782/1‑140 (MQ=255)                                     aTCGAAAATGGCGGGGAGATCGGGGACAAGTGACATGATTTTCCCTCCCCGTACCACGGGAGAAAATGAAAAAAGGCCACATTATTACCCTTCTTTTTATGTGGGTTTTTTTATCTCTCTCCCGGGTTGCCACTTTGttt                                                                                               >  2:1035161/1‑140 (MQ=255)                                     aTCGAAAATGGCGGGGAGATCGGGGACAAGGGGCATGATTTTTCTTCCCCCTACCACGGGGGGGAAAGAAAAAAGGCCACATTATTACCATTCTTTTAGTGTGGGTTTTTTGATCTCTCTCGCGGTTAGCCACTTAGttt                                                                                               >  2:1080800/1‑140 (MQ=255)                                     aTCGAAAATGGCGGGGAGATCGGGGACAAGGGACATGATTTTTCTTCCCCCTACCACGGGAGATAATGAAAAGAGGGCACATTATTACCATTCTTTTTGTGTGGGTTTTTTGATCTCTCTCGCGGGTAGCCACTTAGgtt                                                                                               >  2:1169327/1‑140 (MQ=255)                                     aTCGAAAATGGCAGGTAGATCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGttt                                                                                               >  1:817922/1‑140 (MQ=255)                                         aaaaTGGCGGGTAGATCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCa                                                                                           <  2:1446548/140‑1 (MQ=255)                                                            tGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTGCCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGcc                                                                        <  2:671654/140‑1 (MQ=255)                                                            tGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGcc                                                                        >  1:1144785/1‑140 (MQ=255)                                                            tGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGcc                                                                        >  2:645695/1‑140 (MQ=255)                                                             gACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAAATGCCg                                                                       <  1:1280569/140‑1 (MQ=255)                                                              aCAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGa                                                                      <  1:628119/140‑1 (MQ=255)                                                              aCAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGa                                                                      <  2:1319469/140‑1 (MQ=255)                                                                aaGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAgg                                                                    <  2:1512165/140‑1 (MQ=255)                                                                aaGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAgg                                                                    <  2:89572/140‑1 (MQ=255)                                                                aaGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAgg                                                                    >  2:841697/1‑140 (MQ=255)                                                                aaGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAgg                                                                    <  1:1411850/140‑1 (MQ=255)                                                                 aGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGACATTTAGTTTTTCATGGATTTCTAAAAATTGCCGAggg                                                                   >  2:100369/1‑140 (MQ=255)                                                                  gTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAgggg                                                                  >  2:1024149/1‑140 (MQ=255)                                                                  gTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAgggg                                                                  >  1:603152/1‑140 (MQ=255)                                                                  gTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAgggg                                                                  >  1:29819/1‑140 (MQ=255)                                                                       aTGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACa                                                             <  2:1088762/140‑1 (MQ=255)                                                                            tttCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGcc                                                        <  1:1191357/140‑1 (MQ=255)                                                                             ttccttccCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCg                                                       >  1:143683/1‑140 (MQ=255)                                                                                    cccGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTg                                                >  2:986780/1‑140 (MQ=255)                                                                                    cccGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTg                                                >  1:323867/1‑140 (MQ=255)                                                                                    cccGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTg                                                >  1:557539/1‑140 (MQ=255)                                                                                      cGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCg                                              >  1:795518/1‑140 (MQ=255)                                                                                      cGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCg                                              >  2:181708/1‑140 (MQ=255)                                                                                       gTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGa                                             <  2:318978/140‑1 (MQ=255)                                                                                       gTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGa                                             <  2:318977/140‑1 (MQ=255)                                                                                         aCCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACa                                           >  1:1319153/1‑140 (MQ=255)                                                                                         aCCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACa                                           >  1:1387978/1‑140 (MQ=255)                                                                                            aCGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                        <  1:1445127/140‑1 (MQ=255)                                                                                            aCGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                        <  1:1453782/140‑1 (MQ=255)                                                                                            aCGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                        <  2:972234/140‑1 (MQ=255)                                                                                             cGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCaa                                       >  1:411106/1‑140 (MQ=255)                                                                                             cGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGA‑‑TCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAat                                     >  1:261228/1‑140 (MQ=255)                                                                                             cGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTGGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCaa                                       >  2:1120156/1‑140 (MQ=255)                                                                                              gggAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCaaa                                      >  1:300817/1‑140 (MQ=255)                                                                                               ggAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAat                                     >  1:1489916/1‑140 (MQ=255)                                                                                                  gaTAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAAtata                                  >  2:134052/1‑140 (MQ=255)                                                                                                   aTAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATaa                                 >  1:777691/1‑140 (MQ=255)                                                                                                      aTGAAAAGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAAt                              <  1:1385008/140‑1 (MQ=255)                                                                                                          aaaGAGGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGc                          >  1:243330/1‑140 (MQ=255)                                                                                                            agagGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGccc                        >  1:600552/1‑140 (MQ=255)                                                                                                            agagGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGccc                        >  1:1302587/1‑140 (MQ=255)                                                                                                             gagGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCa                       >  1:324341/1‑140 (MQ=255)                                                                                                              agGCCACATTATTATCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCaa                      >  1:482565/1‑140 (MQ=255)                                                                                                              agGCCACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCaa                      >  2:1107614/1‑140 (MQ=255)                                                                                                                gCCACATTATTACCGTTCATTGCGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCACGGTTTTCTAAAAATTGCCGAGGGGGAAAAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCtata                    >  2:1216654/1‑140 (MQ=255)                                                                                                                 ccACATTATTACCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATAc                   <  2:1061417/140‑1 (MQ=255)                                                                                                                        attaCCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGcc            >  2:781866/1‑140 (MQ=255)                                                                                                                          taCCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCaa          <  1:337335/140‑1 (MQ=255)                                                                                                                           aCCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAAc         <  1:181708/140‑1 (MQ=255)                                                                                                                           aCCATTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAAc         <  2:777691/140‑1 (MQ=255)                                                                                                                              aTTCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAg      >  2:1029493/1‑140 (MQ=255)                                                                                                                               ttCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGc     >  1:707238/1‑140 (MQ=255)                                                                                                                               ttCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGc     >  2:627494/1‑140 (MQ=255)                                                                                                                               ttCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGc     >  2:12243/1‑140 (MQ=255)                                                                                                                               ttCTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGc     >  1:711220/1‑140 (MQ=255)                                                                                                                                 cTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGt   >  2:69259/1‑140 (MQ=255)                                                                                                                                 cTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGt   >  1:707386/1‑140 (MQ=255)                                                                                                                                 cTTTTAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGt   >  1:48336/1‑140 (MQ=255)                                                                                                                                  ttttAGTGTGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTa  >  1:1323639/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     GGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTA  >  NC_000913/4565779‑4566047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑